MSH3 Antibody

Western Blot: MSH3 Antibody [NBP2-19417] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MSH3 Antibody [NBP2-19417] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human colon carcinoma, using MSH3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MSH3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human MSH3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSH3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 127 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for MSH3 Antibody

• Divergent upstream protein
• DNA mismatch repair protein Msh3
• DUC1
• DUG
• DUPMGC163306
• hMSH3
• Mismatch repair protein 1
• MRP1MGC163308
• mutS (E. coli) homolog 3
• mutS homolog 3 (E. coli)

Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSH3 Antibody (NBP2-19417)(2)

Publications using NBP2-19417

• DAmaso E, GonzAlez-Acosta M, Vargas-Parra G et al. Comprehensive Constitutional Genetic and Epigenetic Characterization of Lynch-Like Individuals Cancers (Basel) 2020-07-05 [PMID: 32635641] (IF/IHC, Human)
• Amaral-Silva GK, Sanchez-Romero C, Wagner VP et al. Prognostic Significance of hMSH2, hMSH3 and hMSH6 Expression in Ameloblastoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2017-06-07 [PMID: 28734697] (Human)

Bioinformatics

• Gene Symbol: MSH3
• Uniprot:
  • P20585(Human)