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AHNAK Antibody (EM-09) [DyLight 755]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clone
EM-09
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-62204AF350
Alexa Fluor 405
NBP2-62204AF405
Alexa Fluor 488
NBP2-62204AF488
Alexa Fluor 532
NBP2-62204AF532
Alexa Fluor 594
NBP2-62204AF594
Alexa Fluor 647
NBP2-62204AF647
Alexa Fluor 700
NBP2-62204AF700
Alexa Fluor 750
NBP2-62204AF750
Biotin
NBP2-62204B
DyLight 350
NBP2-62204UV
DyLight 405
NBP2-62204V
DyLight 488
NBP2-62204G
DyLight 550
NBP2-62204R
DyLight 594
NBP2-62204DL594
DyLight 650
NBP2-62204C
DyLight 680
NBP2-62204FR
FITC
NBP2-62204F
HRP
NBP2-62204H
Janelia Fluor 549
NBP2-62204JF549
Janelia Fluor 646
NBP2-62204JF646
Unconjugated
NBP2-62204
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AHNAK Antibody (EM-09) [DyLight 755] Summary

Immunogen
Bacterially expressed fragment of N-terminal domain of human AHNAK1.
Specificity
The antibody C-51 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AHNAK
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for AHNAK Antibody (EM-09) [DyLight 755]

  • AHNAK nucleoprotein (desmoyokin)
  • AHNAK nucleoprotein
  • AHNAK-related
  • AHNAKRS
  • desmoyokin
  • MGC5395
  • neuroblast differentiation-associated protein AHNAK
  • PM227

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of Dysferlin on human skeletal muscle lysates using anti-Dysferlin antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Dysferlin Antibody (JF05-54) [NBP2-66814] - Staining Dysferlin in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Dysferlin Antibody (JF05-54) [NBP2-66814] - Analysis of paraffin-embedded mouse spleen tissue using anti-Dysferlin antibody. Counter stained with hematoxylin.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
S100A10 Antibody
[Unconjugated]
AF2377
Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(11 Publications)
Species: Mu
Applications: WB, Simple Western, IHC
Annexin A2
Antibody [Unconjugate
AF3928
Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A2 at approximately 39 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Annexin A2 was detected in immersion fixed paraffin-embedded sections of normal human liver using Goat Anti-Human/Mouse/Rat Annexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3928) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A2 at approximately 45 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
Desmoplakin
Antibody
NBP1-49879
Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
S100B Antibody
(15F9NB)
NBP2-45267
Western Blot: S100B Antibody (15F9NB) [NBP2-45267] - Total protein from human brain (1) and mouse brain (2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: S100B Antibody (15F9NB) [NBP2-45267] - Analysis in human prostate tissue.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Periaxin Antibody
NBP1-89598
Immunohistochemistry-Paraffin: Periaxin Antibody [NBP1-89598] - Staining of human lung shows strong membranous positivity in pneumocytes.
Immunohistochemistry-Frozen: Periaxin Antibody [NBP1-89598] - Staining of Periaxin in mouse sciatic nerve using anti-Periaxin antibody (green). Image from verified customer review.
Immunohistochemistry-Paraffin: Periaxin Antibody [NBP1-89598] - Analysis in human lung and liver tissues. Corresponding PRX RNA-seq data are presented for the same tissues.
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(1 Publication)
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Periplakin Antibody
NBP2-49306
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Species: Hu
Applications: WB, IHC, IHC-P
Moesin Antibody
(MSN/491)
NBP2-32875
Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Analysis using the Azide and BSA Free version of NBP2-32875. Detection of Moesin in human HT29 Cells.
Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/491) [NBP2-32875] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Fomalin-paraffin Rat colon stained with Moesin MAb (MSN/491)
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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Protocadherin-1
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H00005097-M01
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Calpain 3 Antibody
NBP1-31756
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Immunocytochemistry/Immunofluorescence: Calpain 3 Antibody [NBP1-31756] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
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Glutathione S
Transferase kappa 1 A ...
NBP2-80461
Western Blot: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Western blot analysis of GSTK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: SK-BR-3 cell lysateLane 2: Human skin tissue lysateLane 3: Human placenta tissue lysate
Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GSTK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Flow cytometric analysis of GSTK1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1:100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
SLC30A1 Antibody
NBP2-94196
Western Blot: SLC30A1 Antibody [NBP2-94196] - Analysis of extracts of mouse kidney, using SLC30A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunohistochemistry-Paraffin: SLC30A1 Antibody [NBP2-94196] - Rat kidney using SLC30A1 antibody (40x lens).
Immunohistochemistry-Paraffin: SLC30A1 Antibody [NBP2-94196] - Human esophagus using SLC30A1 antibody (40x lens).
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VAMP4 Antibody
NBP2-13512
Immunocytochemistry/Immunofluorescence: VAMP4 Antibody [NBP2-13512] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: VAMP4 Antibody [NBP2-13512] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells
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Plectin Antibody
NBP1-84019
Immunocytochemistry/Immunofluorescence: Plectin Antibody [NBP1-84019] - Staining of human cell line U-251 MG shows localization to cytosol, intermediate filaments and focal adhesion sites. Antibody staining is shown in green.
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Immunohistochemistry-Paraffin: Plectin Antibody [NBP1-84019] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
S100A1 Antibody
(4C4.9)
NBP2-29403
Western Blot: S100A1 Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 Ab (4C4.9).
Immunohistochemistry-Paraffin: S100A1 Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100 4C4.9 antibody at 1:200 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining.
(3 Publications)
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SLCO6A1 Antibody
NBP2-49118
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: IHC, IHC-P
EGF [Unconjugated]
236-EG
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
(21 Reviews)
(458 Publications)
Species: Hu
AHNAK Antibody
(EM-09) [DyLight 755]
NBP2-62204IR
AHNAK Antibody (EM-09) [DyLight 755]
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AHNAK Antibody (NBP2-62204IR) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-62204AF350
Alexa Fluor 405 NBP2-62204AF405
Alexa Fluor 488 NBP2-62204AF488
Alexa Fluor 532 NBP2-62204AF532
Alexa Fluor 594 NBP2-62204AF594
Alexa Fluor 647 NBP2-62204AF647
Alexa Fluor 700 NBP2-62204AF700
Alexa Fluor 750 NBP2-62204AF750
Biotin NBP2-62204B
DyLight 350 NBP2-62204UV
DyLight 405 NBP2-62204V
DyLight 488 NBP2-62204G
DyLight 550 NBP2-62204R
DyLight 594 NBP2-62204DL594
DyLight 650 NBP2-62204C
DyLight 680 NBP2-62204FR
DyLight 755 NBP2-62204IR
FITC NBP2-62204F
HRP NBP2-62204H
Janelia Fluor 549 NBP2-62204JF549
Janelia Fluor 646 NBP2-62204JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol AHNAK