RP1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: RP1 Antibody [NBP2-22149] - RP1 antibody was tested in ARPE-19 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: RP1 Antibody [NBP2-22149] - IHC analysis of RP1 in mouse retina.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2.0 mg/ml

Order Details

RP1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human RP1 protein (between residues 2000-2156) [UniProt P56715]
Localization
Cytoplasm.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This RP1 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 240 kDa. In ICC/IF cytoplasmic staining was observed in Arpe-19 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse. Predicted to react with monkey based upon 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
2.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RP1 Antibody

  • DCDC4A
  • FLJ50293
  • FLJ55454
  • oxygen-regulated protein 1
  • retinitis pigmentosa 1 (autosomal dominant)
  • retinitis pigmentosa 1 protein
  • retinitis pigmentosa RP1 protein

Background

Retinitis pigmentosa 1 (RP1), also known as oxygen-regulated protein 1, retinitis pigmentosa 1 protein, ORP1, and DCDC4A, regulates the stability and length of microtubule based axoneme of photoreceptors. RP1 is critical for the differentiation of photoreceptor cells, and RP1 also influences the photosensitivity and outer segment morphogenesis of rod photoreceptors. Similarly, RP1 controls the correct orientation and higher order stacking of the outer segment disks along the photoreceptor axoneme. RP1 is found in the cytoplasm and has only been reported in the retina. RP1 is subject to phosphorylation, and mutations in RP1 cause autosomal recessive and dominant retinitis pigmentosa. Both of these diseases lead to degeneration of the retinal photoreceptor cells resulting in night blindness, loss of peripheral visual field, and eventually loss of central vision (PMID: 10391211).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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RP1 NBP2-22149

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Bioinformatics

Gene Symbol RP1
Entrez
Uniprot