| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60531SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49536SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB200-607-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-31123SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86893-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33407-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72569 | |||
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunohistochemistry-Paraffin-NB110-60531-img0011.jpg "Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.")
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0013.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0014.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Western-Blot-NB110-60531-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE (WUE-4) [NB110-60531] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Immunocytochemistry-Immunofluorescence-NB110-60531-img0010.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - Analysis of apoB-positivity in blood plasma and PLT concentrate-derived EXOs. FCM detection of the indicated markers in EXOs conjugated onto latex beads. The EXOs were isolated from fasting PFP or PLT concentrate by differential UC and gravity size filtration (gray histograms: blocked beads incubated with antibody, empty histograms: EXO sample). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep24316), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0020.jpg)
![Flow (Intracellular): Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Intracellular-NB110-60531-img0012.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0015.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on HepG2 cells with Apolipoprotein E/ApoE Antibody NB110-60531 [WUE-4] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0016.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NB110-60531AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0017.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE Antibody [WUE-4] NB110-60531F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0018.jpg)
![Flow Cytometry: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - An intracellular stain was performed on SK-MEL-28 cells with Apolipoprotein E/ApoE [WUE-4] Antibody NB110-60531PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-WUE-4-Flow-Cytometry-NB110-60531-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49536-img0010.jpg "Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- (NBP1-49536) at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Analysis of Pyruvate Carboxylase in A. Human liver extracts and B. Mouse liver extracts](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP1-49536-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse kidney using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a diffused cytoplasmic staining in all the tubules and a subset of cells in glomerular cells.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-49536-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - Characterization of MPC1-/- in the RM-1 murine prostate cancer cells. Western blotting results of subcellular proteins from WT and MPC1-/- cells and corresponding densitometry histograms of ALT1 and Pyruvate Carboxylase. Cyto means cytoplasmic protein. Mito stands for mitochondrial protein. Protein alpha-Tublin was used as a loading control. ***P<0.001. All experiments were performed at least three times with consistent and repeatable results. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18199), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP1-49536-img0012.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP1-49536] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse adipose tissue using rabbit anti-Pyruvate Carboxylase antibody at 1:200 dilution. The signal was developed using HRP conjugated anti-rabbit secondary antibody and DAB reagent. The nuclei were counterstained using hematoxylin. This Pyruvate Carboxylase antibody generated a specific staining of the fat cells in the tested section.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-49536-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP1-49536] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Pyruvate Carboxylase at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49536-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP1-49536] - HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. Divergent effects of human cytomegalovirus and herpes simplex virus-1 on cellular metabolism. PLoS Pathog (2011)](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Knockdown-Validated-NBP1-49536-img0011.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0008.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-NB600-919-img0005.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0001.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, incubation - overnight at 4C.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0002.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Analysis using the Biotin conjugate of NB600-919. Detection of Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptav](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0004.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0007.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-Paraffin-NB600-919-img0003.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - A. 30 ug HepG2 whole cell lysate/extract 12% SDS-PAGE Apolipoprotein E antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0032.jpg "Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - A. 30 ug HepG2 whole cell lysate/extract 12% SDS-PAGE Apolipoprotein E antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP1-31123] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31123-img0033.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (NBP1-31123).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP1-31123-img0036.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells B : Control with 3ug of pre-immune rabbit IgG C : Immunoprecipitation of Apolipoprotein E by 3 ug of Apolipoprotein E antibody [C2C3], C-term 10% SDS-PAGE The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term diluted at 1 : 1000. Easy Blot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0006.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term diluted at 1:5000.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0007.jpg)
![Western Blot: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Sample (2. 5 ug of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Western-Blot-NBP1-31123-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31123-img0008.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Human hepatoma. Apolipoprotein E antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-Immunohistochemistry-Paraffin-NBP1-31123-img0026.jpg)
![ELISA: Apolipoprotein E/ApoE Antibody [NBP1-31123] - Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using as capture antibody at concentration of 5 ug/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 ug/mL. Mouse IgG antibody (HRP) (NBP2-19382) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Apolipoprotein-E-ApoE-Antibody-ELISA-NBP1-31123-img0035.jpg)
![Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Western-Blot-NBP1-49529-img0004.jpg "Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.")
![Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-49529-img0005.jpg)
![Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-Frozen-NBP1-30032-img0006.jpg "Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0001.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0003.jpg)
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in heterozygous (ABCA4+/-) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Western-Blot-NBP1-30032-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in affected (ABCA4-/-) retina. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0005.jpg)
![Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression & western blot analyses of ABCA4 protein levels in the canine retina.(A) Relative ABCA4 mRNA expression levels by quantitative RT-PCR in three different regions in three dogs with different genotypes (ABCA4+/+, ABCA4+/-, & ABCA4-/-), normalized to GAPDH expression. (B) Western blot analyses of ABCA4 (above), GAPDH (middle), & RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202415371970.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-3102024161606.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616064.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-31020241616097.jpg)
![Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30032_mouse-monoclonal-abca4-antibody-3f4-310202416175238.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0012.jpg "Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - mouse kidney. FACL4 stained by FACL4 antibody [C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: FACL4 Antibody [NBP2-16401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16401-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16401] - analysis of FACL4 in MCF10A cells using anti-FACL4 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunofluorescence-NBP2-16401-img0005.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Rat middle brain. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0008.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16401] - Mouse lymph node. FACL4 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16401-img0009.jpg)
![Immunoprecipitation: FACL4 Antibody [NBP2-16401] - Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 ug of FACL4 antibody [C3], C-term Western blot analysis was performed using FACL4 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunoprecipitation-NBP2-16401-img0007.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunohistochemistry-paraffin-261120251821100.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-261120251832454.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunocytochemistry-immunofluorescence-2611202518263624.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-26112025193395.jpg)
![FACL4 antibody [C3], C-term detects FACL4 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.FACL4 stained by FACL4 antibody [C3], C-term (NBP2-16401) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunohistochemistry-paraffin-261120251841135.jpg)
![HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-13220261739389.jpg)
![Fer-1 treatment attenuated changes in ferroptosis-related proteins’ expression in HG-stimulated 661W cells after 18 h. (A) Western blot analysis of ferroptosis-related proteins’ expression levels in HG-induced 661W cells after Fer-1 treatment. GAPDH was used as a control. (B) The downregulation in GPX4 and SLC7A11 protein expression in HG-stimulated 661W cells was significantly attenuated after Fer-1 treatment. The upregulation in ACSL4, FTH1, and NCOA4 protein expression in HG-stimulated 661W cells was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after Fer-1 administration. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-132202617595722.jpg)
![Fer-1 administration attenuated changes of ferroptosis-related proteins expression in diabetic mice retinas. (A) Western blot analysis of ferroptosis-related proteins expression levels in diabetic mice retinas after Fer-1 treatment. GAPDH was used as a control. (B) The decrease in GPX4 and SLC7A11 protein expression in diabetic mice retinas was significantly attenuated after Fer-1 treatment. The increase in ACSL4, FTH1, and NCOA4 protein expression in diabetic mice retinas was effectively abrogated after Fer-1 treatment. (C) Immunofluorescence staining of ferroptosis-related proteins (red) and nuclear (blue) in diabetic mice retinas after Fer-1 treatment: ganglion cell layer (GCL), inner nuclear layer (INL), outer nuclear layer (ONL), inner segment (IS), outer segment (OS). Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-western-blot-13220261755394.jpg)
![HG causes changes in the expression of ferroptosis-related proteins in 661W cells. (A) Western blot analysis of ferroptosis-related protein expression levels in HG-induced 661W cells. GAPDH was used as a control. (B) Expression of GPX4 and SLC7A11 proteins was significantly downregulated in HG-stimulated 661W cells after 12, 18, and 24 h. HG induced obvious upregulation in the expression of ACSL4, FTH1, and NCOA4 in 661W cells compared with the Ctrl group. (C) Immunofluorescence staining of localization of ferroptosis-related proteins (red) and nuclear (blue) in HG-induced 661W cells after 18 h. Data are shown as mean +/- SEM, n = 3 per group for Western blotting. p = not significant [ns], * p < 0.05, ** p < 0.01, *** p < 0.001 versus Ctrl group. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/24/23/16946), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16401_rabbit-polyclonal-facl4-antibody-immunocytochemistry-immunofluorescence-132202619213323.jpg)
![Immunocytochemistry/Immunofluorescence: TRP2 Antibody [NBP1-86893] - Mouse melanoblasts were incubated for 1h at 37C with anti-Trp2 rabbit 1:200, followed by incubation with Donkey anti-rabbit AF488. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86893-img0016.jpg "Immunocytochemistry/Immunofluorescence: TRP2 Antibody [NBP1-86893] - Mouse melanoblasts were incubated for 1h at 37C with anti-Trp2 rabbit 1:200, followed by incubation with Donkey anti-rabbit AF488. ICC/IF image submitted by a verified customer review.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human cerebral cortex, lymph node, skin and testis using Anti-TRP2 antibody NBP1-86893 (A) shows similar protein distribution across tissues to independent antibody NBP1-86892 (B).](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0015.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0018.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human skin shows cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0011.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0013.jpg)
![Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/TRP2-Antibody-Immunohistochemistry-Paraffin-NBP1-86893-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TRP2 Antibody [NBP1-86893] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.](http://images.novusbio.com/fullsize/TRP2-Antibody-Western-Blot-NBP1-86893-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16400-img0002.jpg "Immunocytochemistry/Immunofluorescence: FACL4 Antibody [NBP2-16400] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FACL4 protein stained by FACL4 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded mouse kidney. FACL4 antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0011.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0001.jpg)
![Western Blot: FACL4 Antibody [NBP2-16400] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-Western-Blot-NBP2-16400-img0007.jpg)
![Immunohistochemistry-Paraffin: FACL4 Antibody [NBP2-16400] - Paraffin-embedded rat duodenum. FACL4 antibody [N1N3] diluted at 1:500](http://images.novusbio.com/fullsize/FACL4-Antibody-Immunohistochemistry-Paraffin-NBP2-16400-img0010.jpg)
![Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251827016.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-261120251823622.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518204710.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [N1N3] (NBP2-16400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16400_rabbit-polyclonal-facl4-antibody-western-blot-2611202518422918.jpg)
![Western Blot: Pyruvate Carboxylase Antibody [NBP2-33407] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Western-Blot-NBP2-33407-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Pyruvate Carboxylase Antibody [NBP2-33407] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33407-img0004.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Analysis in human liver and lymph node tissues. Corresponding PC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human cerebellum shows strong granular cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0008.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0009.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Carboxylase Antibody [NBP2-33407] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Pyruvate-Carboxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-33407-img0010.jpg)
![Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Western-Blot-NBP2-45718-img0017.jpg "Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.")
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0016.jpg)
![Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MAFB antibody.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Flow-Cytometry-NBP2-45718-img0001.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0002.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0003.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0004.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0005.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0006.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0007.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0008.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0009.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0010.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0011.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0012.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0013.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0014.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0015.jpg)
![Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.](http://images.novusbio.com/fullsize/FACL4-Antibody-3I8D10-Immunoprecipitation-NBP3-16012-img0002.jpg "Immunoprecipitation: FACL4 Antibody (3I8D10) [NBP3-16012] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug FACL4 antibody (NBP3-16012). Western blot was performed from the immunoprecipitate using FACL4 antibody (NBP3-16012) at a dilition of 1:2000.")
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-5220251718945.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of various lysates using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184534.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Rat liver using FACL4 Rabbit mAb at 1:58000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517202918.jpg)
![Western Blot: FACL4 Antibody (4W3T9) [FACL4] - Western blot analysis of lysates from Hep G2 cells using FACL4 Rabbit mAb at 1:58000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517184563.jpg)
![Immunocytochemistry/ Immunofluorescence: FACL4 Antibody (4W3T9) [FACL4] - Confocal imaging of Hep G2 cells using FACL4 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172885.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Rat brain using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213510.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517213524.jpg)
![Immunohistochemistry: FACL4 Antibody (4W3T9) [FACL4] - Immunohistochemistry analysis of paraffin-embedded Human kidney using FACL4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16012_rabbit-facl4-mab-4w3t9-52202517172887.jpg)