Pyruvate Carboxylase Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bovine, Porcine, RatSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit

Pyruvate Carboxylase Antibody [DyLight 550] Summary

Immunogen
A genomic peptide made to an internal region of the human Pyruvate Carboxylase protein (within residues 930-1050). [Swiss-Prot P11498]
Localization
Mitochondrion matrix.
Predicted Species
Rat (92%), Porcine (96%), Bovine (96%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
PC
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 550
Format
DyLight 550 (A=562, E=576)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:200
Application Notes
This Pyruvate Carboxylase antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~129 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse. Immunogen has 96% identity to bovine and porcine. Rat reactivity reported in scientific literature (PMID: 24333689)

Alternate Names for Pyruvate Carboxylase Antibody [DyLight 550]

  • EC 6.4.1
  • EC 6.4.1.1
  • PCBPyruvic carboxylase
  • pyruvate carboxylase
  • pyruvate carboxylase, mitochondrial

Background

Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pyruvate Carboxylase Antibody (NBP1-49536R) (0)

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Secondary Antibodies

 

Isotype Controls

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