| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55244SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59680SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-26387SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-153SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70648 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59320-25ug | |||
![Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.](http://images.novusbio.com/fullsize/INSIG-1-Antibody-Western-Blot-NB110-55244-img0002.jpg "Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.")
![Immunocytochemistry/Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/INSIG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55244-img0003.jpg)
![Western Blot: Irisin/FNDC5 Antibody [NBP2-59680] - Recombinant human Irisin protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Irisin/FNDC5 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Western-Blot-NBP2-59680-img0003.jpg "Western Blot: Irisin/FNDC5 Antibody [NBP2-59680] - Recombinant human Irisin protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Irisin/FNDC5 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-59680] - IHC analysis of a formalin fixed and paraffin embedded human kidney tissue section (NBP1-78269) using 1:200 dilution of Irisin/FNDC5 antibody. The signal was developed with HRP-DAB detection and the sections were counterstained with hematoxylin. The antibody generated a membrane cytoplasmic staining in almost all of the cell types in the tested section.](http://images.novusbio.com/fullsize/Irisin-FNDC5-Antibody-Immunohistochemistry-Paraffin-NBP2-59680-img0002.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0007.jpg "Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI (whey protein isolate), WPH (whey peptide hydrolysate), BPI (BSA protein isolate) or BPH (BSA peptide hydrolysate). Immunoblotting and densitometric analysis of (A,D) adiponectin (n = 6). mRNA levels of (F) adiponectin (n = 8) were determined. * p < 0.05, **** p < 0.0001 vs. protein isolate controls. PS, protein stain. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32041341) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of Adiponectin was performed using 10% confluent 3T3-L1 cells differentiated with Adipogenesis supplement for 5 days.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunocytochemistry-Immunofluorescence-NBP2-22450-img0011.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed on biopsies of deparaffinized Human skin tissue.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunohistochemistry-Paraffin-NBP2-22450-img0010.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed using 10 ul of conditioned media of Hep G2 cell line (lane 1).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0009.jpg)
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Biopsies of deparaffinized Human skin tissue.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunohistochemistry-NBP2-22450-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Adipocytes isolated from the pelvic area of the male mouse. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-22450_mouse-monoclonal-adiponectin-acrp30-antibody-19f1-immunocytochemistry-immunofluorescence-1111202494957..jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0021.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15336-img0001.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0012.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0015.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0018.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Human gastric cancer, using ALDH1A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15336-img0002.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0007.jpg "Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color")
![Immunohistochemistry: CPT1B Antibody [NBP1-59576] - searcher: Dr. Pankaj Kumar Singh, UNMC, Omaha, NE Application: IHC Species+tissue/cell type:Species+tissue/cell type: Human Capan1 cells Primary antibody dilution: 1:300 Secondary antibody: Anti-rabbit Alexa Fluor 488 Secondary antibody dilution:1:200](http://images.novusbio.com/fullsize/CPT1B-Antibody-Immunohistochemistry-NBP1-59576-img0004.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0003.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0006.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Western Blot: Adropin Antibody [NBP1-26387] - Total protein from Human and Mouse brain was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-Adropin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Adropin-Antibody-Western-Blot-NBP1-26387-img0004.jpg "Western Blot: Adropin Antibody [NBP1-26387] - Total protein from Human and Mouse brain was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-Adropin in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: Adropin Antibody [NBP1-26387] - Western blot on Adropin overexpression lysate.](http://images.novusbio.com/fullsize/Adropin-Antibody-Western-Blot-NBP1-26387-img0003.jpg)
![Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Western-Blot-NB400-153-img0006.jpg "Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - A549 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Immunocytochemistry-Immunofluorescence-NB400-153-img0005.jpg)
![Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.](http://images.novusbio.com/fullsize/FXR-Antibody-Western-Blot-NB400-153-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Immunocytochemistry-Immunofluorescence-NB400-153-img0004.jpg)
![Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Immunohistochemistry-NB100-57851-img0001.jpg "Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Flow Cytometry: DGAT2 Antibody [NB100-57851] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DGAT2-Antibody-Flow-Cytometry-NB100-57851-img0006.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0017.jpg "Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunocytochemistry-Immunofluorescence-H00200576-M01-img0012.jpg)
![Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Immunohistochemistry-Paraffin-H00200576-M01-img0014.jpg)
![Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Western-Blot-H00200576-M01-img0015.jpg)
![Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIKFyve-PIP5K3-Antibody-6C7-Sandwich-ELISA-H00200576-M01-img0016.jpg)
![Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] Analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX2 monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Western-Blot-NBP2-02948-img0011.jpg "Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] Analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunocytochemistry-Immunofluorescence-NBP2-02948-img0010.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human prostate tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0009.jpg)
![Western Blot: EPHX2 Antibody (1A6) [NBP2-02948] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Western-Blot-NBP2-02948-img0001.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0002.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0003.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human endometrium tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0004.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human Kidney tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0005.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human liver tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0006.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human lymphoma tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0007.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody (1A6) [NBP2-02948] - Staining of paraffin-embedded Human pancreas tissue using anti-EPHX2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EPHX2-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-02948-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0007.jpg "Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-59320_mouse-monoclonal-sur1-antibody-s289-16-310202416114773.jpg)