EPHX2 Antibody (OTI1A6) - Azide and BSA Free

Western Blot: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2.

Immunocytochemistry/Immunofluorescence: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human pancreas tissue using anti-EPHX2 mouse monoclonal antibody.

Western Blot: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX2 monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPHX2 mouse monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPHX2 mouse monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human endometrium tissue using anti-EPHX2 mouse monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human Kidney tissue using anti-EPHX2 mouse monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human liver tissue using anti-EPHX2 mouse monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human lymphoma tissue using anti-EPHX2 mouse monoclonal antibody.

Immunohistochemistry: EPHX2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-70648] - Staining of paraffin-embedded Human prostate tissue using anti-EPHX2 mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: OTI1A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

EPHX2 Antibody (OTI1A6) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPHX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 62.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for EPHX2 Antibody (OTI1A6) - Azide and BSA Free

• Bifunctional Epoxide Hydrolase 2
• CEH
• Cytosolic epoxide hydrolase
• EC 3.3.2.10
• EPHX2
• Epoxide Hydratase 2
• Epoxide hydratase
• Epoxide Hydrolase 2
• epoxide hydrolase 2, cytoplasmic
• Epoxide Hydrolase 2, Cytosolic
• epoxide hydrolase, soluble
• SEH
• Soluble epoxide hydrolase

Background

EPHX2 encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHX2