DGAT2 Antibody

Immunohistochemistry-Paraffin: DGAT2 Antibody [NB100-57851] - Analysis of DGAT2 in Human Liver. Antibody at 3 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Flow Cytometry: DGAT2 Antibody [NB100-57851] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DGAT2 Antibody Summary

• Description: Novus Biologicals Goat DGAT2 Antibody (NB100-57851) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-DGAT2 Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KLEHPTQQDIDLYH corresponding to internal region (near C-Terminus) according to NP_115953.2.
• Specificity: This antibody is not expected to cross-react with other DGAT members.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DGAT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunohistochemistry 3 ug/mL
  • Immunohistochemistry-Paraffin 3 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot
• Application Notes: Use in Western blot reported in scientific literature (PMID: 30928647).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24500776). Mouse reactivity reported in scientific literature (PMID: 30928647).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DGAT2 Antibody

• DGAT2
• diacylglycerol O-acyltransferase 2
• diacylglycerol O-acyltransferase homolog 2 (mouse)
• diacylglycerol O-acyltransferase homolog 2
• diacylglycerol O-acyltransferase-like protein 2
• Diglyceride acyltransferase 2
• DKFZp686A15125
• EC 2.3.1
• EC 2.3.1.20
• GS1999FULL

Background

Acyl-CoA:diacylglycerol acyltransferase, or DGAT (EC 2.3.1.20), is responsible for the synthesis of triglycerides. It catalyzes a reaction in which diacylglycerol is covalently joined to long chain fatty acyl-CoAs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGAT2 Antibody (NB100-57851)(9)

Publications using NB100-57851

• Yamamoto T, Maurya SK, Pruzinsky E et al. RIP140 deficiency enhances cardiac fuel metabolism and protects mice from heart failure The Journal of clinical investigation 2023-03-16 [PMID: 36927960] (WB, Mouse)
  
  Details:
  Dilution used in WB 1:1000
• S�nchez-Ceinos J, Guzm�n-Ruiz R, Rangel-Z��iga OA et al. Impaired mRNA splicing and proteostasis in preadipocytes in obesity-related metabolic disease eLife 2021-09-21 [PMID: 34545810]
• Miotto PM, Petrick HL, Holloway GP Acute insulin deprivation results in altered mitochondrial substrate sensitivity conducive to greater fatty acid transport Am. J. Physiol. Endocrinol. Metab. 2020-06-16 [PMID: 32543943]
• Maria Guarino, Pavitra Kumar, Andrea Felser, Luigi M. Terracciano, Sergi Guixé-Muntet, Bostjan Humar, Michelangelo Foti, Jean-Marc Nuoffer, Marie V. St-Pierre, Jean-François Dufour Exercise Attenuates the Transition from Fatty Liver to Steatohepatitis and Reduces Tumor Formation in Mice Cancers 2020-05-29 [PMID: 32486073]
• Hucik B, Sarr O, Nakamura MT et al. Reduced delta-6 desaturase activity partially protects against high-fat diet-induced impairment in whole-body glucose tolerance J. Nutr. Biochem. 2019-03-02 [PMID: 30928647] (WB, Mouse)
• Fukushima A, Zhang L, Huqi A et al. Acetylation contributes to hypertrophy-caused maturational delay of cardiac energy metabolism JCI Insight 2018-05-17 [PMID: 29769443]
• Beaudoin MS, Snook LA, Arkell AM et al. Novel effects of rosiglitazone on SMAD2 and SMAD3 signaling in white adipose tissue of diabetic rats. Obesity (Silver Spring). 2014-02-06 [PMID: 24500776] (WB, Rat)
• Cases S, Stone SJ, Zhou P et al. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. J Biol Chem 2001-10-19 [PMID: 11481335]
• Hucik-Worndl B Investigating Diet and Exercise Modifications on Delta-6 Desaturase and Ghrelin Regulation of Skeletal Muscle and White Adipose Tissue Lipid Metabolism Thesis 2021-01-01

Bioinformatics

• Gene Symbol: DGAT2
• Entrez:
  • 84649(Human)
  • 67800(Mouse)
  • 252900(Rat)
• Uniprot:
  • Q96PD7(Human)