| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27216 | |||
| Azide and BSA Free | NBP2-25199SS | |||
| Azide and BSA Free | NBP2-27216-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-31106-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1985SS | |||
| Azide and BSA Free | NBP2-80657 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-64979-0.05mg | |||
| Azide Free | NB100-64979SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24827SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92695SS | |||
![Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0007.jpg "Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATR Antibody (2B5) [NB100-308] - Raji whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ATR-Antibody-2B5-Western-Blot-NB100-308-img0005.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0007.jpg "Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-27216. Staining of human PBMC. Image from verified customer review.")
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in human PBMC using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0004.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the FITC conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0011.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the DyLight 405 conjugate of NBP2-27216. Staining of CD4 in human PBMCs using anti-CD4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0005.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the PE conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of this antibody. Green represents isotype control; red represents anti-CD4 antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0009.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using the Allophycocyanin conjugate of NBP2-27216. Staining of CD4 in 1x10^6 human PBMC using 10 ul (0.1 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/CD4-Antibody-RPA-T4-Flow-Cytometry-NBP2-25199-img0010.jpg)
![Flow Cytometry: CD4 Antibody (RPA-T4) - Azide and BSA Free [NBP2-25199] - analysis of CD4 in human PBMCs using 0.1 ug of this antibody. Secondary antibody is goat anti-mouse PE.](http://images.novusbio.com/fullsize/nbp2-25199_mouse-monoclonal-cd4-antibody-rpa-t4-imgenex-img-5916a-25ug-17720241044654.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0005.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Action potentials were evoked with depolarizing current pulses. (A) Neuron from colonic specimen of non-treated patient fired 1 action potential in response to a depolarizing current. (A') Intracellular injection of carboxyfluorescein during recording confirmed Dogiel type I, uniaxonal morphology. (A")
![Immunohistochemistry-Paraffin: nNOS Antibody [NB100-858] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunohistochemistry-Paraffin-NB100-858-img0006.jpg)
![Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nNOS-Antibody-Flow-Cytometry-NB100-858-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a-2d) and 7 (4a-4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a-1d) and 7 (3a-3d) days. Myenteric ganglia and neurons labeled with beta-Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of nNOS neurons (green) can be seen at both Days 3 and 7 after oxaliplatin treatment. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fnins.2013.00030/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: nNOS Antibody [NB100-858] - (A) Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a–2d) & 7 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a–1d) & 7 (3a–3d) days. Myenteric ganglia & neurons labeled with beta -Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of NOS neurons (green) can be seen at both Days 3 & 7 after oxaliplatin treatment. Scale bar = 20 μm. (B) Wholemount preparations of the myenteric neurons in the distal colon segments following 14 (2a–2d) & 21 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 14 (1a–1d) & 21 (3a–3d) days. The reduction in total number of neurons & increase in the proportion of NOS neurons was found at both Days 14 & 21 after oxaliplatin treatment. Scale bar = 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23486839), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-858_goat-polyclonal-nnos-antibody-310202416284925.jpg)
![Immunocytochemistry/ Immunofluorescence: nNOS Antibody [NB100-858] - (A) Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a–2d) & 7 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a–1d) & 7 (3a–3d) days. Myenteric ganglia & neurons labeled with beta -Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of NOS neurons (green) can be seen at both Days 3 & 7 after oxaliplatin treatment. Scale bar = 20 μm. (B) Wholemount preparations of the myenteric neurons in the distal colon segments following 14 (2a–2d) & 21 (4a–4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 14 (1a–1d) & 21 (3a–3d) days. The reduction in total number of neurons & increase in the proportion of NOS neurons was found at both Days 14 & 21 after oxaliplatin treatment. Scale bar = 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23486839), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-858_goat-polyclonal-nnos-antibody-310202416284920.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0012.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Western-Blot-NBP2-31106-img0008.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0010.jpg)
![Flow Cytometry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on A431 cells with CD47 Antibody [B6H12.2] NBP2-31106PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Flow-Cytometry-NBP2-31106-img0009.jpg)
![Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then probed with 2 ug/ml of anti-CD47. CD47 protein was detected using an anti-mouse HRP secondary antibody.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Western-Blot-NBP2-31106-img0004.jpg)
![Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-NBP2-31106-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] conjugated to Biotin (NBP2-31106B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0011.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0003.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0002.jpg)
![Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Flow-Cell-Surface-NBP2-31106-img0006.jpg)
![Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Western-Blot-NB100-1985-img0001.jpg "Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Western blot of CD74 from IP N87 lysates mixed with macrophage migration inhibitory factor (MIF).")
![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human spleen using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Immunohistochemistry-Paraffin-NB100-1985-img0004.jpg)
![Flow Cytometry: CD74 Antibody (PIN.1) [NB100-1985] - CD74 in human PBMCs (gated on all monocytes). Orange represents isotype control and blue represents anti-CD74 antibody staining.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Flow-Cytometry-NB100-1985-img0006.jpg)
![Western Blot: CD74 Antibody (PIN.1) [NB100-1985] - Ramos whole cell protein was separated on a 4-12% gel by SDS-PAGE and protein transferred to PVDF. The membrane was probed with anti-CD74 at 2 ug/ml and detected with and anti-mouse HRP secondary antibody using chemiluminescence. The arrow head shows the detection of CD74.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Western-Blot-NB100-1985-img0005.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Immunohistochemistry-Paraffin-NB100-1985-img0002.jpg)
![Immunohistochemistry-Paraffin: CD74 Antibody (PIN.1) [NB100-1985] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human pulmonary squamous cell carcinoma using mouse monoclonal CD74 antibody (clone PIN.1) at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/CD74-Antibody-PIN-1-Immunohistochemistry-Paraffin-NB100-1985-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24893-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - Intracellular analysis of mouse TLR 6 polyclonal antibody (Red peak), rabbit isotype control (Green peak), RAW cells (Purple peak).Two micrograms of antibodies were used. Goat anti-rabbit FITC was used as secondary antibody.")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24893] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24893-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Immunocytochemistry-Immunofluorescence-NB100-64979-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - Analysis of TfR (Transferrin R) Antibody on mouse brain endothelial cells. Mouse brain endothelial (bEnd.3) cells expressing Transferrin (FICT, green) and nuclei (DAPI, blue). Experimental details: 0.1 % triton, BSA 30 min, Primary Ab: 1:50, 1h, RT, Secondary Ab: AlexaFluor 488 chicken anti-rat IgG (H+L), 1:800, 30 min. Image from verified customer review.")
![Flow Cytometry: TfR (Transferrin R) Antibody (8D3) - Azide Free [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-8D3-Flow-Cytometry-NB100-64979-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-891-img0006.jpg "Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NB300-891-img0002.jpg)
![Flow Cytometry: FLAP Antibody [NB300-891] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FLAP-Antibody-Flow-Cytometry-NB300-891-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-891-img0004.jpg)
![Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. Antibody at 6 ug/mL.](http://images.novusbio.com/fullsize/FLAP-Antibody-Immunohistochemistry-Paraffin-NB300-891-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0015.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Staining (6ug/ml) of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0016.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NB100-2833] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NB100-2833-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia and neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), and neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) and 4 (NeuN) experimental mice. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0204941), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2833-img0014.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB100-2833] - Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NB100-2833-img0017.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Pg/gingipain is present intracellularly in astrocytes, microglia & neurons in the hippocampus of experimental mice.Green: astrocytes (top panels), microglia (middle panels), & neurons (bottom panels). Red: Pg/gingipain. Blue: DAPI. Representative of N = 4 (GFAP), 3 (Iba1) & 4 (NeuN) experimental mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-3102024153430.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-31020241535694.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - Proinflammatory cytokines IL6, IL1 beta & TNF alpha are evident in the hippocampus of experimental but not in control mice.Data from IF microscopy & RT-PCR. (A-F) Results from IF microscopy. IL6 expression in control (A) & experimental (B) mice, IL1 beta expression in control (C) & experimental (D) mice & TNF alpha expression in control (E) & experimental (F) mice. N = 3 mice/group. (G-I) Gene expression of cytokines was detected by RT-PCR (G, H, I). In all cases, there is significantly higher gene expression of all three cytokines in experimental compared with control group (G, H, I). Green: Iba1, Red: cytokines, Blue: DAPI. N = 5 mice/group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30281647), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-310202415363735.jpg)
![Immunocytochemistry/ Immunofluorescence: AIF-1/Iba1 Antibody [NB100-2833] - IL-1 beta synthesis, & microglial activation modulation in PDD005-treated aged mice. (A) Images showing IL-1 beta (green fluorescence), iba-1 (red fluorescence) expression. Yellow = Overlay; Blue = nuclei; SGZ region of mouse brain. 40X magnification, scale bar = 100 μm. (B) Graphs representing the average relative fluorescence of Iba-1 in SGZ. Significant differences determined by using one-way ANOVA with Tukey’s test. *P > Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26536027), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2833_goat-polyclonal-aif-1-iba1-antibody-31020241553740.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16908-img0041.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0032.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0035.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0036.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0014.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16908_rabbit-polyclonal-aif-1-iba1-antibody-910202419593573.jpg)
![Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TLR5-Antibody-Western-Blot-NBP2-24827-img0004.jpg "Western Blot: TLR5 Antibody [NBP2-24827] - THP-1 cell lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TLR5 Antibody [NBP2-24827] - Analysis of TLR5 in FFPE human stomach tumor tissue using TLR5 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/TLR5-Antibody-Immunohistochemistry-Paraffin-NBP2-24827-img0002.jpg)
![Flow Cytometry: TLR5 Antibody [NBP2-24827] - Intracellular flow analysis of TLR5 in Balb/c mouse splenocytes using TLR5 antibody at 2 ug/10^6 cells. Shaded histogram: cells without antibody. Green: rabbit IgG isotype control. Red: TLR5 antibody. Goat anti-rabbit PE was used as secondary antibody.](http://images.novusbio.com/fullsize/TLR5-Antibody-Flow-Cytometry-NBP2-24827-img0003.jpg)
![Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Western-Blot-NBP1-92695-img0005.jpg "Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.")
![Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.](http://images.novusbio.com/fullsize/TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0003.jpg)
![Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0006.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunoprecipitation-NBP1-92695-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0008.jpg)