GPER/GPR30 Antibody

Images

 
Western Blot: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with rat hypothalamus 5-10ug protein loaded per lane. Image courtesy provided by Carrie McAllister.
Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.

Product Details

Summary
Reactivity Hu, Rt, Mk, Pm, Ca, Ha, OpSpecies Glossary
Applications WB, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GPER/GPR30 Antibody Summary

Immunogen
Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR30.
Localization
Endoplasmic reticulum - multi-pass membrane protein.
Specificity
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Canine (94%), Opossum (94%), Hamster (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPER
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry-Paraffin 6.4 ug/ml
  • Immunofluorescence 1:10 - 1:500
Application Notes
IP usage is reported in scientific literature (PMID: 19531209). ELISA usage is reported in scientific literature (PMID: 18489713).
Reviewed Applications
Read 1 Review rated 4
using
NLS4271 in the following applications:

Publications
Read Publications using
NLS4271 in the following applications:

  • 3 publications
  • 1 publication
  • IHC
    2 publications
  • 1 publication
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Gorilla, Marmoset. Predicted cross-reactivity based on sequence identity: Elephant (94%), Panda (88%), Bat (88%), Equine (88%), Rabbit (88%), Platypus (88%). Rat reactivity reported via customer review. Mouse reactivity reported by a customer in PMID 23273253.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GPER/GPR30 Antibody

  • CEPR
  • CEPRG-protein coupled receptor 30
  • Chemoattractant receptor-like 2
  • chemokine receptor-like 2
  • CMKRL2
  • CMKRL2MGC99678
  • constitutively expressed peptide-like receptor
  • DRY12
  • DRY12IL8-related receptor DRY12
  • FEG-1
  • FEG-1mER
  • Flow-induced endothelial G-protein coupled receptor 1
  • G protein-coupled estrogen receptor 1
  • G protein-coupled receptor 30
  • GPCR-BR
  • GPER
  • GPR30
  • GPR30GPCR-Br
  • G-protein coupled estrogen receptor 1
  • heptahelix receptor
  • LERGU
  • LERGU2
  • leucine rich protein in GPR30 3'UTR
  • LyGPR
  • Lymphocyte-derived G-protein coupled receptor
  • Membrane estrogen receptor

Background

GPR30 is a transmembrane intracellular estrogen receptor (Revankar et al. 2005). Progestin inhibits growth in breast cancer cells through GPR30. GPR30 has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. ESTs have been isolated from B-cell/lung/testis, brain, ganglion, heart/melanocyte/uterus, kidney, tonsil, liver/spleen, placenta, and prostate libraries. Ligands: Estrogen (Revankar et al. 2005). Caution: Rat GPR30 is also known as GPR41 and HIA-R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GPER/GPR30 Antibody (NLS4271)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ELISA
(3)
ICC/IF
(1)
IHC
(2)
IHC-P
(1)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(5)
All Species
Showing Publications 1 - 10 of 10.
Publications using NLS4271 Applications Species
Tica AA, Tica OS, Georgescu CV et al. GPER and ERalpha expression in abnormal endometrial proliferations. Rom J Morphol Embryol. Aug 12 2016 [PMID: 27516013] (IHC, Human) IHC Human
Jala VR, Radde BN, Haribabu B, Klinge CM. Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer. BMC Cancer 2012 Dec 28 [PMID: 23273253] (WB, IHC-P, Human, Mouse) WB, IHC-P Human, Mouse
Li Q, Sullivan NR, McAllister CE, Van de Kar LD, Muma NA. Estradiol accelerates the effects of fluoxetine on serotonin 1A receptor signaling Psychoneuroendocrinology 2012 Dec 4 [PMID: 23219224] (WB, Rat) WB Rat
McAllister CE, Creech RD, Kimball PA, Muma NA, Li Q. GPR30 is necessary for estradiol-induced desensitization of 5-HT(1A) receptor signaling in the paraventricular nucleus of the rat hypothalamus. Psychoneuroendocrinology. 2012 Jan 18. [PMID: 22265196] (WB, Rat) WB Rat
Maiti K, Paul JW, Read M et al. G-1-activated membrane estrogen receptors mediate increased contractility of the human myometrium Endocrinology 2011 Jun [PMID: 21427217] (WB, IHC, ICC/IF, Human) WB, IHC, ICC/IF Human
Jeng YJ, Kochukov M, Watson CS Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells. Environ Health. 2010 Oct 15 [PMID: 20950447] (ELISA, Rat) ELISA Rat
Park II, Zhang Q, Liu V et al. 17{beta}-estradiol at low concentrations acts through distinct pathways in normal versus BPH-derived prostate stromal cells. Endocrinology :en.2008-1591. 2009 [PMID: 19608654]
Alyea RA, Watson CS. Nongenomic mechanisms of physiological estrogen-mediated dopamine efflux. BMC Neurosci. 2009 Jun 16 [PMID: 19531209] (IP, ELISA, Rat) IP, ELISA Rat
Alyea RA, Watson CS. Differential regulation of dopamine transporter function and location by low concentrations of environmental estrogens and 17beta-estradiol. Environ Health Perspect. 2009 May [PMID: 19479021] (WB, Human) WB Human
Alyea RA, Laurence SE, Kim SH et al. The roles of membrane estrogen receptor subtypes in modulating dopamine transporters in PC-12 cells. J Neurochem. 2008 Aug [PMID: 18489713] (WB, ELISA, Rat) WB, ELISA Rat

Review for GPER/GPR30 Antibody (NLS4271) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NLS4271:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GPER/GPR30 NLS4271
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reviewed by:
Anonymous
WB Rat 01/24/2013
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Summary

ApplicationWestern Blot
Sample Testedrat hypothalamus
SpeciesRat
Lot8967

Blocking

Blocking Details5% milk in TBS with 1% Tween, 1 hr at room temperature

Primary Anitbody

Dilution Ratio1:1000 in 2% milk (TBS-Tween) for 48hr at 4 degrees

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Jackson ImmunoResearch #111-035-003
Secondary Concentration1:10000

Details

Detection NotesECL - Pierce Dura substrate, 15 minute exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GPER/GPR30 Antibody (NLS4271). (Showing 1 - 1 of 1 FAQs).

  1. What is the MW band size for GPER with your GPR30 Antibody (NLS4271)?
    • The expected molecular weight of GPR30 is 42.3, however, because this protein has at least 3 different glycosylation sites, you should not be surprised if you see a band at higher position in your samples.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
01/24/2013
Application: WB
Species: Rat

Bioinformatics

Gene Symbol GPER
Entrez
Uniprot