GPR18 Antibody - BSA Free

Western Blot: GPR18 Antibody [NBP2-24918] - Analysis of human spleen lysate (4 ug/mL) in the 1) absence and 2) presence of immunizing peptide 3) mouse spleen (6 ug/mL) and 4) rat spleen lysate (3 ug/mL) using GPR18 antibody.

Immunocytochemistry/Immunofluorescence: GPR18 Antibody [NBP2-24918] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-GPR18 Antibody NBP2-24918 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of a FFPE tissue section of human tonsil using 1:200 dilution of GPR18 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on Daudi cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunohistochemistry-Paraffin: GPR18 Antibody [NBP2-24918] - Analysis of human breast tumor tissue using an isotype control (top) and GPR18 antibody (bottom) at 5 ug/mL.

Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on Ntera2 cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: GPR18 Antibody [NBP2-24918] - An intracellular stain was performed on THP-1 cells with GPR18 Antibody NBP2-24918 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPR18 Antibody - BSA Free Summary

• Immunogen: Partial synthetic peptide made to an N-terminal portion of the human GPR18 protein (between amino acids 1-50) [UniProt Q14330]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPR18
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 27256622)
  • Immunocytochemistry/ Immunofluorescence 2 - 10 ug/ml. Use reported in scientific literature (PMID 30894369)
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR18 Antibody - BSA Free

• G protein-coupled receptor 18
• GPCRW
• GPR18
• G-protein coupled receptor 18
• NAGly Receptor
• N-arachidonyl glycine receptor

Background

GPR18 is an Orphan-A GPCR with no known ligand. GPR18 has been reported to be expressed at high levels in testis and spleen and at lower levels in other tissues associated with endocrine and immunologic/hematologic functions. ESTs have been isolated from human blood, bone marrow, brain, colon, liver/spleen, lymph, lymph node, testis, and tonsil libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPR18 Antibody (NBP2-24918)(6)

Publications using NBP2-24918

• Yildiz O, Hunt GP, Schroth J et Al. Lipid-mediated resolution of inflammation and survival in amyotrophic lateral sclerosis Brain Commun 2025-01-01 [PMID: 39816195]
• Anghelache M, Voicu G, Deleanu M et al. Biomimetic Nanocarriers of Pro-Resolving Lipid Mediators for Resolution of Inflammation in Atherosclerosis Advanced healthcare materials 2023-10-18 [PMID: 37852632] (FLOW, Mouse)
• Alqahtani S, Xia L, Jannasch A Et al. Disruption of pulmonary resolution mediators contribute to exacerbated silver nanoparticle-induced acute inflammation in a metabolic syndrome mouse model Toxicology and applied pharmacology 2021-11-15 [PMID: 34601004]
• Sharma M, Schlegel MP, Afonso MS et al Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression Circ Res 2020-04-28 [PMID: 32336197] (ICC/IF, ICC/IF, Mouse)
  
  Details:
  Citation using the DyLight 650 version of this antibody.
• Bhatia S, Oweida A, Lennon S et al. Inhibition of EphB4-ephrin-B2 signaling reprograms the tumor immune microenvironment in head and neck cancers Cancer Res. 2019-03-20 [PMID: 30894369] (ICC/IF, Human)
• Croasdell A, Sime PJ, Phipps RP Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes FASEB J. 2016-09-30 [PMID: 27256622] (FLOW, Human)

Review for GPR18 Antibody (NBP2-24918) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/23/2019

Summary

• Application: Western Blot
• Sample Tested: HT-29 human colon adenocarcinoma cell line,Jurkat
• Species: Human
• Lot: 06113013C

FAQs for GPR18 Antibody (NBP2-24918). (Showing 1 - 1 of 1 FAQs).

1. Has the antibody been tested in Flow Cytometry?
   • Our GPR18 antibody with catalogue number NBP2-24918 has been tested for Western blotting and for IHC with paraffin-embedded tissue samples, but it has not yet been tested in any other applications. For your future reference, if we have tested one of our antibodies in a particular application and found it not to work, we would indicate this by writing (negative) after that application. In this instance you might wish to participate in our Innovator's Reward programme. This was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Further details can be found at the following link: Innovators Reward.

Bioinformatics

• Gene Symbol: GPR18