Adenosine A1R Antibody - BSA Free

Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).

Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.

Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left).

Flow Cytometry: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Adenosine A1R Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1.
• Localization: Cell Membrane
• Specificity: Detects Adenosine Receptor A1. This does not detect other AR subtypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADORA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3 - 5 ug / 10^6 cells
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500
• Application Notes: Adenosine A1R antibody validated for ICC/IF from a verified customer review. Use in IHC- Frozen reported in scientific literature (PMID: 24016034).
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18948751). Rat from customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Adenosine A1R Antibody - BSA Free

• Adenosine A1 R
• adenosine A1 receptor
• Adenosine A1R
• ADORA 1
• ADORA1
• RDC7
• RDC7adenosine receptor A1

Background

Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenosine A1R Antibody (NB300-549)(8)

Publications using NB300-549

• Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters 2021-09-16 [PMID: 34536555] (FLOW, Human)
• Kutryb-Zajac B, Jablonska P, Serocki M et al. Extracellular Adenine Nucleotide and Adenosine Metabolism in Calcific Aortic Valve Disease. bioRxiv 2018-08-28 [PMID: 32126886] (ICC/IF, Human)
• Kutryb-Zajac B, Mierzejewska P, Sucajtys-Szulc E et al. Inhibition of LPS-stimulated ecto-adenosine deaminase attenuates endothelial cell activation J. Mol. Cell. Cardiol. 2019-01-11 [PMID: 30641086] (ICC/IF, Human)
• Hackett TA. Adenosine A1 Receptor mRNA Expression by Neurons and Glia in the Auditory Forebrain. Anat Rec (Hoboken). 2018-10-12 [PMID: 30315630] (IF/IHC, Mouse)
• Ren ZH, Lin CZ, Cao W et al. CD73 is associated with poor prognosis in HNSCC. Oncotarget. 2016-09-20 [PMID: 27557512] (IF/IHC, WB, Human)
• Yamaguchi D, Terayama R, Omura S et al. Effect of adenosine A1 receptor agonist on the enhanced excitability of spinal dorsal horn neurons after peripheral nerve injury. Int J Neurosci. 2013-10-14 [PMID: 24016034] (IHC-Fr, Rat)
• Yu, W, Zacharia, L C, Jackson, E K, Apodaca, G. Adenosine receptor expression function in bladder uroepithelium, . 291, C254-265. 2006-01-01 [PMID: 16571869]
• Kimura M, Saitoh N, Takahashi T et al. Adenosine A(1) receptor-mediated presynaptic inhibition at the calyx of Held of immature rats. J Physiol. 2003-12-01 [PMID: 12963795]

Review for Adenosine A1R Antibody (NB300-549) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer ICC Rat 08/27/2014

Summary

• Application: Immunocytochemistry
• Species: Rat

Bioinformatics

• Gene Symbol: ADORA1
• Entrez:
  • 29290(Rat)
• Uniprot:
  • P25099(Rat)