Adenosine A1R Antibody - BSA Free Summary
Immunogen |
Synthetic peptide corresponding to residues C(309) Q P K P P I D E D L P E E K A E D(326) of rat Adenosine Receptor A1. |
Localization |
Cell Membrane |
Specificity |
Detects Adenosine Receptor A1. This does not detect other AR subtypes. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
ADORA1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 3 - 5 ug / 10^6 cells
- Immunocytochemistry/Immunofluorescence 2 ug/ml
- Immunohistochemistry 1:10 - 1:500
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin 1:50 - 1:200
- Western Blot 1:500
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Application Notes |
Adenosine A1R antibody validated for ICC/IF from a verified customer review. Use in IHC- Frozen reported in scientific literature (PMID: 24016034). |
Theoretical MW |
37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reviewed Applications |
Read 1 Review rated 4 using NB300-549 in the following applications:
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Publications |
Read Publications using NB300-549 in the following applications:
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Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 18948751). Rat from customer review.
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for Adenosine A1R Antibody - BSA Free
Background
Adenosine receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily. Structural, biochemical and pharmacological analyses of the AR genes and protein has led to the discovery of four distinct AR subtypes (A1, A2a, A2b, A3). Activation of ARs mediates several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission.The A1AR is a glycoprotein that can activate Gi and Go proteins in vitro. In intact cells, agonist occupation of the A1AR has been shown to cause pertussis toxin-sensitive inhibition of adenylyl cyclase activity and, in some systems, a stimulation of phospholipase C resulting in mobilization of intracellular calcium stores. Activation of potassium channels by A1AR has been intensively studied in relation to its dramatic effects on the cardiovascular system. A1AR protein is highly expressed in brain (especially cerebellum, hippocampus, thalamus, and cortex) and spinal cord and in part, modulates neurotransmitter release. In white adipocytes A1AR inhibits lipolysis and stimulates glucose uptake. Other tissues also express A1AR including kidney and testis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
FAQs for Adenosine A1R Antibody (NB300-549) (0)
Secondary Antibodies
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Isotype Controls
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