| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88220-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87528-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43347-0.5mg | |||
| BSA Free | NBP1-43347-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61931SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92711SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47940-0.1mg | |||
| IHC-Prediluted | NBP2-48115 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64843SS | |||
| Azide and BSA Free | NBP2-80574 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-538-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-407SS | |||
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum, skeletal muscle, stomach and testis using Anti-CALB2 antibody NBP1-88220 (A) shows similar protein distribution across tissues to independent antibody NBP1-88221 (B).](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0020.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum, skeletal muscle, stomach and testis using Anti-CALB2 antibody NBP1-88220 (A) shows similar protein distribution across tissues to independent antibody NBP1-88221 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining in human testis and skeletal muscle tissues using NBP1-88220 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0024.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0023.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0021.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0022.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Filaggrin Antibody [NBP1-87528] - Staining of human cell line HaCaT shows localization to vesicles.](http://images.novusbio.com/fullsize/Filaggrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87528-img0021.jpg "Immunocytochemistry/Immunofluorescence: Filaggrin Antibody [NBP1-87528] - Staining of human cell line HaCaT shows localization to vesicles.")
![Western Blot: VE-Cadherin Antibody (BV14) [NBP1-43347] - Non-reduced (left) and reduced (right) bEnd.3 cell line lysates were loaded at 1x10^5 cells/lane, probed with 2 ug/mL of Anti-Mouse CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.](http://images.novusbio.com/fullsize/VE-Cadherin-Antibody-BV14-Western-Blot-NBP1-43347-img0003.jpg "Western Blot: VE-Cadherin Antibody (BV14) [NBP1-43347] - Non-reduced (left) and reduced (right) bEnd.3 cell line lysates were loaded at 1x10^5 cells/lane, probed with 2 ug/mL of Anti-Mouse CD144 (VE-Cadherin) Purified and revealed with Anti-Rat IgG HRP.")
![Western Blot: Cytokeratin 5 Antibody [NBP2-61931] - Total protein from human A431 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cytokeratin 5 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-Western-Blot-NBP2-61931-img0002.jpg "Western Blot: Cytokeratin 5 Antibody [NBP2-61931] - Total protein from human A431 cells and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cytokeratin 5 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody [NBP2-61931] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.1% Triton X-100. The cells were incubated with anti-Cytokeratin 5 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61931-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody [NBP2-61931] - Analysis of a FFPE tissue section of human skin using 1:1000 dilution of rabbit anti-Cytokeratin 5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Cytokeratin 5 antibody generated a specific membrane cytoplasmic staining in the epithelial cells of the skin tissue. The signal was highest in the keratinocytes while dermis was largely negative for this Cytokeratin 5 (KRT5).](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-61931-img0003.jpg)
![Flow Cytometry: Cytokeratin 5 Antibody [NBP2-61931] - An intracellular stain was performed on A431 cells with Cytokeratin 5 Antibody NBP2-61931AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-5-Antibody-Flow-Cytometry-NBP2-61931-img0004.jpg)
![Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Immunocytochemistry-Immunofluorescence-NBP1-92711-img0001.jpg "Immunohistochemistry Free-Floating: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of rat hippocampus section stained with mouse mAb to MAP2, NBP1-92711, dilution 1:5,000 in green, and costained with rabbit pAb to alpha-internexin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-92711 antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, and the alpha-internexin antibody selectively stains axons and dendrites of neuronal cells.")
![Western Blot: MAP2 Antibody (5H11) [NBP1-92711] - Analysis of different tissue lysates using mouse mAb to MAP2A/B (5H11), NBP1-92711, dilution 1:10,000 in green: [1] protein standard (red), [2] adult rat whole brain, [2] embryonic (E20) rat brain, [4] adult rat spinal cord, and [5] adult mouse brain lysate. A band observed at about 280 kDa corresponds to full length MAP2a and MAP2B protein. MAP2A/B is expressed heavily in brain particularly in cortical regions, but is a more minor component of spinal cord. Note that the epitope for this antibody in within the projection domain, and so the antibody does not bind to the lower molecular weight MAP2C and MAP2D isoforms which lack this region.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Western-Blot-NBP1-92711-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (5H11) [NBP1-92711] - Single cultured astrocytes stained with MAP2 (red) and Myelin (green). Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-5H11-Immunocytochemistry-Immunofluorescence-NBP1-92711-img0003.jpg)
![Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Western-Blot-NB300-132-img0003.jpg "Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Immunocytochemistry-Immunofluorescence-NB300-132-img0004.jpg)
![Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Simple-Western-NB300-132-img0005.jpg)
![Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Western-Blot-NBP2-44814-img0008.jpg "Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunocytochemistry-Immunofluorescence-NBP2-44814-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44814-img0005.jpg)
![Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Flow-Cytometry-NBP2-44814-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44814-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44814-img0002.jpg)
![Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Simple-Western-NBP2-44814-img0003.jpg)
![Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Simple-Western-NBP2-44814-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Formalin-fixed, paraffin-embedded human prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).](http://images.novusbio.com/fullsize/nbp2-44814_mouse-monoclonal-cytokeratin-7-antibody-ov-tl12-30-17720249362618.jpg)
![Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/nbp2-44814_mouse-monoclonal-cytokeratin-7-antibody-ov-tl12-30-17720241003210.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Immunohistochemistry-Paraffin-NB100-64843-img0007.jpg "Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.")
![Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of mouse peripheral blood granulocytes with rat anti-mouse CD13.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Flow-Cytometry-NB100-64843-img0006.jpg)
![Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Mouse lymph node cryosection anti-CD13 antibody.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Immunohistochemistry-Frozen-NB100-64843-img0003.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of a mouse tail cryosection with anti-mouse CD13 antibody.](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-ER-BMDM1-Immunohistochemistry-Paraffin-NB100-64843-img0004.jpg)
![Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Western-Blot-NB300-538-img0017.jpg "Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0019.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0016.jpg)
![Flow Cytometry: Galectin-3 Antibody (A3A12) [NB300-538] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Flow-Cytometry-NB300-538-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of rat olfactory bulb](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of HeLa cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0013.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0014.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human ovarian carcinoma (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0015.jpg)
![Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546] - Mouse cardiac tissue stained with Troponin I Type 3 antibody. IHC-P image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-Antibody-19C7cc-Immunohistochemistry-Paraffin-NB110-2546-img0003.jpg "Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (19C7cc) [NB110-2546] - Mouse cardiac tissue stained with Troponin I Type 3 antibody. IHC-P image submitted by a verifed customer review.")
![Flow Cytometry: Troponin I Type 3 (cardiac) Antibody (19C7) [NB110-2546] - FACS analysis of Troponin I Type 3 in differentiated ESC cultures using anti-Troponin I Type 3 (cardiac) antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-Antibody-19C7-Flow-Cytometry-NB110-2546-img0002.jpg)
![Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).")
![<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - Analysis of HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate using 14-3-3 gamma antibody at 1:500.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunocytochemistry-Immunofluorescence-NB100-174-img0017.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0019.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0008.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0010.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0011.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0015.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunoprecipitation-NB100-174-img0016.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr1-p84-Thoc1 antibody [5E10] (NB100-174) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-174_mouse-monoclonal-hhpr1-p84-thoc1-antibody-5e10-western-blot-1411202518352219.jpg)