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hHpr1-p84-Thoc1 Antibody (5E10)

 (7 Publications)
 (1 Review)
 
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Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lysate/extract. 7.5% SDS-PAGE. p84 antibody [5E10] dilution: 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug ...read more
Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. ...read more
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: ...read more
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Mouse Cerebellum, whole cell lysate (50 ug), stained with hHpr1-p84-Thoc1 Antibody (5E10), diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7. 5% SDS PAGE; ...read more
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.
Biological Strategies: Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: ...read more
Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clone
5E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

      

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hHpr1-p84-Thoc1 Antibody (5E10) Summary

Immunogen
Amino acids 15-374 of human p84 expressed in E. coli.
Localization
Nuclear Marker
Specificity
This is specific for p84.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
THOC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 2 ug/ml
  • Simple Western
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 0.5 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
Application Notes
Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50.
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
hHpr1-p84-Thoc1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-174 in the following applications:
Publications
Read Publications using
NB100-174 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for hHpr1-p84-Thoc1 Antibody (5E10)

  • HPR1THO complex subunit 1
  • hTREX84
  • Nuclear matrix protein p84
  • P84
  • P84N5
  • THO complex 1
  • tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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hHpr1-p84-Thoc1
Antibody (5E10)
NB100-174
hHpr1-p84-Thoc1 Antibody (5E10)
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for hHpr1-p84-Thoc1 Antibody (NB100-174)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


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Review for hHpr1-p84-Thoc1 Antibody (NB100-174) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-174:
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(1)
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Images Ratings Applications Species Date Details
Western Blot hHpr1-p84-Thoc1 NB100-174
Enlarge 		5
reviewed by:
Anonymous
 WB Human 07/25/2014
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Summary

ApplicationWestern Blot
Sample Tested293T cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for hHpr1-p84-Thoc1 Antibody (NB100-174) (0)

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Control Lysate(s)

hHpr1-p84-Thoc1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Western Blot hHpr1-p84-Thoc1 NB100-174
Enlarge
 Anonymous
07/25/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol THOC1
Entrez