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hHpr1-p84-Thoc1 Antibody (5E10)

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 (1 Review)
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Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Biological Strategies: Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p84 protein stained by p84 antibody [5E10] diluted at ...read more
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Mouse Cerebellum, whole cell lysate (50 ug), stained with hHpr1-p84-Thoc1 Antibody (5E10), diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7. 5% SDS PAGE; ...read more
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, MkSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IP
Clone
5E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-174AF405
Alexa Fluor 488
NB100-174AF488
Alexa Fluor 647
NB100-174AF647
Alexa Fluor 700
NB100-174AF700
Biotin
NB100-174B
DyLight 350
NB100-174UV
DyLight 405
NB100-174V
DyLight 488
NB100-174G
DyLight 550
NB100-174R
DyLight 650
NB100-174C
DyLight 680
NB100-174FR
DyLight 755
NB100-174IR
FITC
NB100-174F
HRP
NB100-174H
Janelia Fluor 549
NB100-174JF549
Janelia Fluor 646
NB100-174JF646
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hHpr1-p84-Thoc1 Antibody (5E10) Summary

Immunogen
Amino acids 15-374 of human p84 expressed in E. coli.
Localization
Nuclear Marker
Specificity
This is specific for p84.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
THOC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:100-1:500
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
hHpr1-p84-Thoc1 Lysate (NBL1-16887)
Reviewed Applications
Read 1 Review rated 5
using
NB100-174 in the following applications:
Publications
Read Publications using
NB100-174 in the following applications:

  • WB
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for hHpr1-p84-Thoc1 Antibody (5E10)

  • HPR1THO complex subunit 1
  • hTREX84
  • Nuclear matrix protein p84
  • P84
  • P84N5
  • THO complex 1
  • tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: IRF9 Antibody [NBP1-81577] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
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cleaved
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant HumanIFN-alpha 2a (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog AF2890), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT2 (Y689) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y689) at approximately 111 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y689) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1, dark orange filled histogram) for 20 minutes, then stained with Rabbit Anti-Human Phospho‑STAT2 (Y689) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2890) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
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Applications: WB, Simple Western, CyTOF-ready, ICFlow
hHpr1-p84-Thoc1
Antibody (5E10)
NB100-174
hHpr1-p84-Thoc1 Antibody (5E10)
Species: Hu, Mu, Rt, Ha, Mk
Applications: WB, ChIP, ICC/IF, IHC, IP

Publications for hHpr1-p84-Thoc1 Antibody (NB100-174)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for hHpr1-p84-Thoc1 Antibody (NB100-174) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-174:
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(1)
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Images Ratings Applications Species Date Details
Western Blot hHpr1-p84-Thoc1 NB100-174
Enlarge
reviewed by:
Anonymous
 WB Human 07/25/2014
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Summary

ApplicationWestern Blot
Sample Tested293T cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for hHpr1-p84-Thoc1 Antibody (NB100-174) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

hHpr1-p84-Thoc1 Overexpression Lysate (Native)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-174AF405
Alexa Fluor 488 Labeled NB100-174AF488
Alexa Fluor 647 Labeled NB100-174AF647
Alexa Fluor 700 Labeled NB100-174AF700
DyLight 350 Labeled NB100-174UV
DyLight 405 Labeled NB100-174V
DyLight 488 Labeled NB100-174G
DyLight 680 Labeled NB100-174FR
DyLight 755 Labeled NB100-174IR
Learn about Custom Antibody Labeling

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Diseases for hHpr1-p84-Thoc1 Antibody (NB100-174)

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Western Blot hHpr1-p84-Thoc1 NB100-174
Enlarge
 Anonymous
07/25/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol THOC1
Entrez