hHpr1-p84-Thoc1 Antibody (5E10)

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lysate/extract. 7.5% SDS-PAGE. p84 antibody [5E10] dilution: 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Mouse Cerebellum, whole cell lysate (50 ug), stained with hHpr1-p84-Thoc1 Antibody (5E10), diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.

Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Ha, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP
• Clone: 5E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

hHpr1-p84-Thoc1 Antibody (5E10) Summary

• Immunogen: Amino acids 15-374 of human p84 expressed in E. coli.
• Localization: Nuclear Marker
• Specificity: This is specific for p84.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: THOC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 0.5 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • Simple Western
  • Western Blot 0.3 - 2 ug/ml
• Application Notes: Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50.
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  hHpr1-p84-Thoc1 Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for hHpr1-p84-Thoc1 Antibody (5E10)

• HPR1THO complex subunit 1
• hTREX84
• Nuclear matrix protein p84
• P84
• P84N5
• THO complex 1
• tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for hHpr1-p84-Thoc1 Antibody (NB100-174)(7)

Publications using NB100-174

• Maeder CI, Kim JI, Liang X et al. The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival Cell 2018-08-21 [PMID: 30146163] (IP, Mouse)
• Chen YJ, Tsai CH, Wang PY, Teng, SC. SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression. Sci Rep. 2017-06-19 [PMID: 28630472] (WB, Human)
• Hsu WB, Shih JL, Shih JR et al. Cellular Protein HAX1 Interacts with the Influenza A Virus PA Polymerase Subunit and Impedes Its Nuclear Translocation J Virol 2013-01-01 [PMID: 23055567] (WB, Human)
• Lopitz-Otsoa F, Rodriguez-Suarez E, Aillet F et al. Integrative analysis of the ubiquitin proteome isolated using Tandem Ubiquitin Binding Entities (TUBEs). J Proteomics 2012-06-06 [PMID: 22178446] (WB, Human)
• Wong CW, Hou PS, Tseng SF et al. Kruppel-like transcription factor 4 contributes to maintenance of telomerase activity in stem cells. Stem Cells 2010-09-01 [PMID: 20629177] (WB, Human)
• Guo, S et al. Linking Transcriptional Elongation Messenger RNA Export to Metastatic Breast Cancers. Cancer Res 2005; 65: (8). 2005-01-01 [PMID: 15833825]
• Bounpheng MA, Melnikova IN, Dodds SG et al. Characterization of the mouse JAB1 cDNA and protein. Gene 2000-01-01 [PMID: 10721695]

Review for hHpr1-p84-Thoc1 Antibody (NB100-174) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Costache Vasilescu WB Human 07/25/2014

Summary

• Application: Western Blot
• Sample Tested: 293T cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: THOC1
• Entrez:
  • 9984(Human)