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hHpr1-p84-Thoc1 Antibody (5E10)

 (7 Publications)
 (1 Review)
 
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Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lysate/extract. 7.5% SDS-PAGE. p84 antibody [5E10] dilution: 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug ...read more
Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. ...read more
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: ...read more
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Mouse Cerebellum, whole cell lysate (50 ug), stained with hHpr1-p84-Thoc1 Antibody (5E10), diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7. 5% SDS PAGE; ...read more
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.
Biological Strategies: Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: ...read more
Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Clone
5E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

      

Order Details

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hHpr1-p84-Thoc1 Antibody (5E10) Summary

Immunogen
Amino acids 15-374 of human p84 expressed in E. coli.
Localization
Nuclear Marker
Specificity
This is specific for p84.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
THOC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 0.5 - 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100-1:500
  • Simple Western
  • Western Blot 0.3 - 2 ug/ml
Application Notes
Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50.
Theoretical MW
84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
hHpr1-p84-Thoc1 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-174 in the following applications:
Publications
Read Publications using
NB100-174 in the following applications:

  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for hHpr1-p84-Thoc1 Antibody (5E10)

  • HPR1THO complex subunit 1
  • hTREX84
  • Nuclear matrix protein p84
  • P84
  • P84N5
  • THO complex 1
  • tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power
Immunofluorescence: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4, green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power
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(2 Reviews)
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-alpha 2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho‑STAT1 (Y701) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
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(2 Reviews)
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NB100-56583
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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(1 Review)
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Immunocytochemistry/Immunofluorescence: STAT2 Antibody (JF0884) [NBP2-67168] - Staining STAT2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: STAT2 Antibody (JF0884) [NBP2-67168] - Staining STAT2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: STAT2 Antibody (JF0884) [NBP2-67168] - Analysis of paraffin-embedded human colon cancer tissue using anti-STAT2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
hHpr1-p84-Thoc1
Antibody (5E10)
NB100-174
hHpr1-p84-Thoc1 Antibody (5E10)
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP

Publications for hHpr1-p84-Thoc1 Antibody (NB100-174)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


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Review for hHpr1-p84-Thoc1 Antibody (NB100-174) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-174:
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Images Ratings Applications Species Date Details
Western Blot hHpr1-p84-Thoc1 NB100-174
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 07/25/2014
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Summary

ApplicationWestern Blot
Sample Tested293T cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for hHpr1-p84-Thoc1 Antibody (NB100-174) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

hHpr1-p84-Thoc1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Additional hHpr1-p84-Thoc1 Products

Bioinformatics Tool for hHpr1-p84-Thoc1 Antibody (NB100-174)

Discover related pathways, diseases and genes to hHpr1-p84-Thoc1 Antibody (NB100-174). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for hHpr1-p84-Thoc1 Antibody (NB100-174)

Discover more about diseases related to hHpr1-p84-Thoc1 Antibody (NB100-174).
 

Pathways for hHpr1-p84-Thoc1 Antibody (NB100-174)

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PTMs for hHpr1-p84-Thoc1 Antibody (NB100-174)

Learn more about PTMs related to hHpr1-p84-Thoc1 Antibody (NB100-174).

Blogs on hHpr1-p84-Thoc1

There are no specific blogs for hHpr1-p84-Thoc1, but you can read our latest blog posts.
Omicron Variant Antibodies

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Contact Information

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Product PDFs

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Calculators

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Recent Reviews

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Western Blot hHpr1-p84-Thoc1 NB100-174
Enlarge
 Verified Customer
07/25/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol THOC1
Entrez