14-3-3 gamma Antibody (HS23)

Images

 
Biological Strategies: Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, ...read more
Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - Analysis of HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate using 14-3-3 gamma antibody at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, ZeSpecies Glossary
Applications WB, IB, ICC/IF
Clone
HS23
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

14-3-3 gamma Antibody (HS23) Summary

Immunogen
N-terminal fragment of human 14-3-3 gamma [Uniprot: P61981]
Localization
Cytoplasmic
Specificity
NB100-407 reacts with the human form of intact, non-modified N-terminus 14-3-3 gamma. The naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated is not recognized by this antibody.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
YWHAG
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
Application Notes
In Western Blot, a distinct band around 33 kDa is detected. Use in immunoblotting reported in scientific literature (PMID: 29317475).
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
14-3-3 gamma Overexpression Lysate
14-3-3 gamma Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-407 in the following applications:

  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for 14-3-3 gamma Antibody (HS23)

  • 1433 gamma
  • 14-3-3 gamma
  • 14-3-3 protein gamma
  • 14-3-3GAMMA
  • KCIP-1
  • Protein kinase C inhibitor protein 1
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gammapolypeptide
  • YWHAG

Background

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 14-3-3 gamma Antibody (NB100-407)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(6)
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Human
(4)
Mouse
(3)
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Showing Publications 1 - 8 of 8.
Publications using NB100-407 Applications Species
Cao T, Jiang Y, Wang Z et al. MetAP2 inhibition increases energy expenditure through direct action on brown adipocytes J. Biol. Chem. May 2 2019 [PMID: 31048375] (WB, Mouse) WB Mouse
Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the Function of Expansion Segments in Ribosomes Mol. Cell Dec 20 2018 [PMID: 30576652] (WB, Mouse) WB Mouse
Jonckheere V, Fijalkowska D, Van Damme P. Omics Assisted N-terminal Proteoform and Protein Expression Profiling On Methionine Aminopeptidase 1 (MetAP1) Deletion. Mol Cell Proteomics 2018 Apr [PMID: 29317475]
Kleppe R, Rosati S, Jorge-Finnigan A et al. Phosphorylation dependence and stoichiometry of the complex formed by tyrosine hydroxylase and 14-3-3-gamma. Mol. Cell Proteomics. 2014 Jun 19 [PMID: 24947669] (WB, Human)

Details:
Native electrophoresis /immunodetection was performed on Tetrameric TH and dimeric 14-3-3 gamma complexes generated by mixing them in 1:3 subunit molar ratio (TH:14-3-3 gamma). 10 uM of purified TH (non-phosphorylated TH, TH-pS19 and TH-pS19pS40) was incubated with 30 uM 14-3-3 gamma for 5 min at room temperature and the samples were processed for WB using 14-3-3 gamma antibody at 1:1000 dilution.
WB Human
Zhang F, Bhat S, Gabelli SB et al. Pyridinylquinazolines selectively inhibit human methionine aminopeptidase-1 in cells. J Med Chem 2013 May 23 [PMID: 23634668] (WB, Human) WB Human
Bhat S, Shim JS, Zhang F et al. Substituted oxines inhibit endothelial cell proliferation and angiogenesis. Org Biomol Chem. 2012 Apr 21 [PMID: 22391578] (WB, Human) WB Human
Shim JS, Matsui Y, Bhat S et al. Effect of nitroxoline on angiogenesis and growth of human bladder cancer. J Natl Cancer Inst 2010 Dec 15 [PMID: 21088277] (WB, Human, Mouse) WB Human, Mouse
Hu, X et al. Elucidation of the function of type 1 human methionine aminopeptidase during cell cycle progression. Proc Natl Acad Sci U S A;103(48):18148-53. 2006 Nov 28. [PMID: 17114291]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol YWHAG
Entrez
Uniprot