| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25156SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-676SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-407SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32932-0.02mg | |||
| Azide and BSA Free | NBP2-34713-0.2mg | |||
| Azide and BSA Free | NBP2-34713-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1832SS | |||
| Azide and BSA Free | NBP2-80962 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33108 | |||
| IHC-Prediluted | NBP2-45167 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-297SS | |||
![Immunohistochemistry-Paraffin: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-FA6-152-Immunohistochemistry-Paraffin-NB600-1423-img0003.jpg "Immunohistochemistry-Paraffin: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human")
![Flow Cytometry: CD36 Antibody (FA6-152) [NB600-1423] - CD36, Human](http://images.novusbio.com/fullsize/CD36-Antibody-FA6-152-Flow-Cytometry-NB600-1423-img0007.jpg)
![Immunohistochemistry-Frozen: CD36 Antibody (FA6-152) [NB600-1423] - Frozen section of tonsil tissue. Staining especially in vascular endothelia.](http://images.novusbio.com/fullsize/CD36-SR-B3-Antibody-FA6-152-Immunohistochemistry-Frozen-NB600-1423-img0001.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Cytometry-NBP1-48348-img0015.jpg "Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - Staining of cytokeratin on guinea pig breast carcinoma.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Immunohistochemistry-Paraffin-NBP1-48348-img0002.jpg)
![Western Blot: Cytokeratin, pan Antibody (C-11) - BSA Free [NBP1-48348] - Analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-BSA-Free-Western-Blot-NBP1-48348-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (C-11) - BSA Free [NBP1-48348] - Staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-48348-img0017.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (C-11) - BSA Free [NBP1-48348] - Intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-BSA-Free-Flow-Intracellular-NBP1-48348-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-pan Cytokeratin (C-11) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Immunocytochemistry-Immunofluorescence-NBP1-48348-img0014.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin (C-11) antibody NBP1-48348 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Intracellular-NBP1-48348-img0005.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody (C-11) PE. Overlay with Isotype mouse IgG1 control.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Cytometry-NBP1-48348-img0007.jpg)
![Flow (Intracellular): Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Intracellular-NBP1-48348-img0010.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on A549 cells with pan Cytokeratin Antibody (C-11) NBP1-48348F488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-C-11-Flow-Cytometry-NBP1-48348-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-25156-img0003.jpg "Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.")
![Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP2-25156 antibody labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunocytochemistry-Immunofluorescence-NBP2-25156-img0005.jpg)
![Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Western-Blot-NBP2-25156-img0006.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of MAP2 in a 1% PFA fixed mouse brain section using anti-MAP2 (red) and NeuN (green) antibodies. Image from verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0004.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0008.jpg)
![Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining of a 1% PFA fixed mouse cortical region section with anti-MAP 2 (dilution 1:300; Alexa 568 red). Image from a verified customer review.](http://images.novusbio.com/fullsize/MAP2-Antibody-4H5-Immunohistochemistry-Paraffin-NBP2-25156-img0007.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0015.jpg "Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY license.")
![Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0012.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/p97-VCP-Antibody-Western-Blot-NB100-1558-img0013.jpg)
![Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20 ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells & asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated & subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue & afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22607136), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1558_rabbit-polyclonal-p97-vcp-antibody-31020241535618.jpg)
![Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Knockout-Validated-NB300-676-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676] - Analysis of UCHL1 in mouse brain lysate ECL detection, 60 seconds.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-676-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676] - Neuro2a cells stained with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-676-img0007.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NB300-676] - Staining of neurons and cell processes at 2.5ug/mL. Human brain, Substantia Nigra, Pigmented Neurons, 4X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NB300-676-img0009.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody [NB300-676] - An intracellular stain was performed on U-87 cells with UCH-L1/PGP9.5 Antibody NB300-676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Flow-Cytometry-NB300-676-img0012.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody [NB300-676] - Image shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB300-676-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunocytochemistry-Immunofluorescence-NB100-174-img0017.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0019.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0008.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0010.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0011.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0015.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunoprecipitation-NB100-174-img0016.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr1-p84-Thoc1 antibody [5E10] (NB100-174) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb100-174_mouse-monoclonal-hhpr1-p84-thoc1-antibody-5e10-western-blot-1411202518352219.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/NF-M-Antibody-Knockout-Validated-NB300-133-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NF-M Antibody [NB300-133] - Analysis lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Rabbit Anti-Human NF-M Polyclonal Antibody (Catalog # NB300-133) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Analysis of rat cerebellum section stained with rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in red, and costained with mouse mAb to GAP43, dilution 1:2,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. NB300-133 antibody strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regios in the molecular layer.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0008.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of neuronal tissue lysates using rabbit pAb to NF-M, NB300-133, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig brain, [7] pig spinal cord. Strong bands at 145kDa correspond to rodent NF-M molecules, while the NF-M of pig and other larger mammals including humans run at about 160kDa.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NB300-133-img0009.jpg)
![Immunohistochemistry-Frozen: NF-M Antibody [NB300-133] - Staining of cerebral cortex section from Rat with NF-M antibody (red) at 1:5000 dilution. This immunostaining reveals the perikarya of pyramidal neurons and dendrites as well as axons surrounding the neurons. The green channel shows staining with a monoclonal antibody to beta-adrendergic receptor kinase 1.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Frozen-NB300-133-img0007.jpg)
![Western Blot: NF-M Antibody [NB300-133] - Analysis of 160kDa Neurofilament Medium expression on whole rat cerebellum homogenate using NB300-133.](http://images.novusbio.com/fullsize/p_165_1.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-133] - Mixed neuron/glia cultures stained with NB300-133 (red) and NBP2-25141 (green), Novus' mouse monoclonal antibody to Fox1, an mRNA binding protein closely related to Fox3/NeuN. The RPCA-NF-M antibody stains axonal, dendritic and perikaryal profiles of neurons cleanly and specifically. Like antibody to Fox3/NeuN, the Fox1 antibody binds to the nuclei of neurons only. DNA is shown in blue with the DAPI stain.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NB300-133-img0005.jpg)
![Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - 14-3-3 gamma detected in lysates using NB 100-407. Lane 1: HeLa cell lysate, Lanes 2 and 3: bengamide treated lysates (8h and 24h, respectively).")
![<b>Biological Strategies Validation. </b>Western Blot: 14-3-3 gamma Antibody (HS23) [NB100-407] - Analysis of HeLa (A), A431 (B), NIH3T3 (C) and PC-12 (D) cell lysate using 14-3-3 gamma antibody at 1:500.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-HS23-Western-Blot-NB100-407-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) [NBP2-32932] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10)](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Immunohistochemistry-Paraffin-NBP2-32932-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - PSC characterisation. Human PSC-1 characterized for typical undifferentiated colony morphology in expression of pluripotency markers Nanog (Catalog # AF1997), OCT-3/4 (Catalog # AF1759), SSEA-3 (NB100-1832), SSEA-4 (Catalog # MAB1435) after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33899-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Immunocytochemistry-Immunofluorescence-NB100-1832-img0016.jpg "Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - PSC characterisation. Human PSC-1 characterized for typical undifferentiated colony morphology in expression of pluripotency markers Nanog (Catalog # AF1997), OCT-3/4 (Catalog # AF1759), SSEA-3 (NB100-1832), SSEA-4 (Catalog # MAB1435) after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33899-6), licensed under a CC-BY license.")
![Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832] - A surface stain was performed on Jurkat cells with SSEA-3 Antibody (MC-631) NB100-1832AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Flow-Cytometry-NB100-1832-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Immunocytochemistry-Immunofluorescence-NB100-1832-img0012.jpg)
![Western Blot: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Western-Blot-NBP2-25219-img0014.jpg "Western Blot: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Western Blot Analysis of HeLa cell lysate using Podocalyxin Like Antibody (3D3).")
![Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Confocal Immunofluorescence of HeLa cells using Podocalyxin Like Antibody (3D3) labeled with CF488 (Green); Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Immunocytochemistry-Immunofluorescence-NBP2-25219-img0011.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Immunohistochemistry-Paraffin-NBP2-25219-img0013.jpg)
![Flow Cytometry: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Surface staining of NCCIT cells using Podocalyxin antibody at 1 ug/10^6 cells. A FITC-conjugated anti mouse Ig secondary antibody was used along with this antibody .](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Flow-Cytometry-NBP2-25219-img0003.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-paraffin angiosarcoma stained with Podocalyxin Ab (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Immunohistochemistry-Paraffin-NBP2-25219-img0005.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (3D3) [NBP2-25219] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Like Antibody (3D3).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-3D3-Immunohistochemistry-Paraffin-NBP2-25219-img0012.jpg)
![Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.](http://images.novusbio.com/fullsize/TERT-Antibody-2D8-Western-Blot-NB100-297-img0010.jpg "Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.](http://images.novusbio.com/fullsize/TERT-Antibody-2D8-Immunocytochemistry-Immunofluorescence-NB100-297-img0009.jpg)
![Immunohistochemistry-Paraffin: TERT Antibody (2D8) [NB100-297] - TERT was detected in immersion fixed paraffin-embedded sections of human liver cancer using anti-human mouse monoclonal antibody (Catalog # NB100-297) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/TERT-Antibody-2D8-Immunohistochemistry-Paraffin-NB100-297-img0011.jpg)