| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-520SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77456SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56368SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89284-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33315 | |||
| IHC-Prediluted | NBP2-44777 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-675SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22125SS | |||
| Azide and BSA Free | NBP2-80752 | |||
![Western Blot: GRP78/HSPA5 Antibody [NB300-520] - GRP78 on indicated cell extracts using NB300-520.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NB300-520-img0004.jpg "Western Blot: GRP78/HSPA5 Antibody [NB300-520] - GRP78 on indicated cell extracts using NB300-520.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NB300-520] - GRP78/BIP antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NB300-520-img0005.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NB300-520] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NB300-520-img0006.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Western-Blot-NBP1-77456-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.")
![Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Immunohistochemistry-NBP1-77456-img0003.jpg)
![Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-Simple-Western-NBP1-77456-img0005.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Noxa Antibody [NB100-56368] - Noxa expression was stably reduced in HCT116 cells by shRNA. Western blot image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Noxa Antibody [NB100-56368] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using Noxa antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunohistochemistry-Paraffin-NB100-56368-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Noxa Antibody [NB100-56368] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Noxa (NB100-56368) antibody at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Noxa-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56368-img0003.jpg)
![Western Blot: Noxa Antibody [NB100-56368] - Western blot analysis of Noxa in A) human, B) mouse, and C) rat thymus tissue using Noxa antibody NB100-56368 at 2 ug/mL.](http://images.novusbio.com/fullsize/Noxa-Antibody-Western-Blot-NB100-56368-img0001.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Immunohistochemistry-Paraffin-NB500-330-img0009.jpg "Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).")
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0007.jpg)
![Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Immunohistochemistry-Frozen-NB500-330-img0008.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0002.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0004.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0003.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0005.jpg)
![Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD59-Antibody-MEM-43-Flow-Cytometry-NB500-330-img0006.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0007.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.")
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0006.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0005.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB110-58870-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1 using NB110-58869. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58870-img0003.jpg)
![Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Simple-Western-NB110-58870-img0004.jpg)
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (100/D5).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5) [NBP2-15200] - Formalin-paraffin non-Hodgkin's lymphoma stained with Bcl-2 Ab (100/D5). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Immunohistochemistry-Paraffin-NBP2-15200-img0005.jpg)
![Flow Cytometry: Bcl-2 Antibody (100/D5) [NBP2-15200] - Intracellular staining of Bcl-2 in fixed and permeabilized Jurkat cells using Bcl-2 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Flow-Cytometry-NBP2-15200-img0002.jpg)
![Western Blot: Bcl-2 Antibody (100/D5) [NBP2-15200] - analysis of Bcl-2 in human skin using Bcl-2 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5-Western-Blot-NBP2-15200-img0004.jpg)
![Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0004.jpg "Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.")
![Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Simple-Western-NBP1-05194-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Western-Blot-NBP1-05194-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-2A7-Immunocytochemistry-Immunofluorescence-NBP1-05194-img0002.jpg)
![Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-2A7-Flow-Cytometry-NBP1-05194-img0006.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of extracts from C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:200). Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-675-img0012.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of extracts from C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:200). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of acetone-fixed, frozen mouse ear skin section using anti-PGP9.5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunofluorescence-NB300-675-img0014.jpg)
![Immunohistochemistry: PGP9.5 / UCHL-1 Antibody [NB300-675] - Human brain, Substantia Nigra, Pigmented Neuron, 40X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-NB300-675-img0010.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB300-675] - Detection of UCHL1 in mouse brain lysate.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-675-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - In Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-675-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - Confocal analysis of C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-675-img0013.jpg)
![Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.](http://images.novusbio.com/fullsize/NF-L-Antibody-Western-Blot-NB300-131-img0004.jpg "Western Blot: NF-L Antibody [NB300-131] - Analysis of different tissue lysates using rabbit pAb to NF-L, NB300-131, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Neurofilament (NF-L antibody) used as axon marker in WT and KO mice using immunofluorescence staining to illustrate the axon morphology of retinal ganglion cells. Central part of retina is enlarged and displayed in WT and KO mice. Scale bars are 500 micrometers for left and 200 micrometers for right. Citation: Lin Y-S, Kuo K-T, Chen S-K, Huang H-S (2018) RBFOX3/NeuN is dispensable for visual function. PLoS ONE 13(2): e0192355. //doi.org/10.1371/journal.pone.0192355](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunofluorescence-NB300-131-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 68kDa Neurofilament Light Antibody [NB300-131] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L RPCA-NF-L (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA](http://images.novusbio.com/fullsize/68kDa-Neurofilament-Light-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NF-L Antibody [NB300-131] - Analysis of mouse cerebellum section stained with rabbit pAb to NF-L, NB300-131, dilution 1:5,000 in red, and costained with chicken pAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. NB300-131 antibody labels dendrites and axons of neuronal cells, and MBP antibody stains the network of myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-L-Antibody-Immunocytochemistry-Immunofluorescence-NB300-131-img0003.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Western-Blot-NBP2-22125-img0003.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.")
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded kidney cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Immunohistochemistry-Paraffin-NBP2-22125-img0006.jpg)
![Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of Jurkat cells using Glucose 6 Phosphate Dehydrogenase mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Flow-Cytometry-NBP2-22125-img0004.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mAb against human Glucose 6 Phosphate Dehydrogenase (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Western-Blot-NBP2-22125-img0002.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded breast cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-Immunohistochemistry-Paraffin-NBP2-22125-img0005.jpg)
![ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Antibody-2H7-ELISA-NBP2-22125-img0001.jpg)
![Simple Western: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) - BSA Free [NBP2-22125] - B[a]P-Induced Changes in Cell Cycle. (a) Beas-2B cells treated with 1 µM or 10 µM B[a]P (24 h), 3 µg/mL aphidicolin (18 h), & 100 ng/mL nocodazole (18 h) were prepared for cell imaging post-treatment & immunofluorescent staining. A reference cell cycle diagram is shown. The categorization schematic applied to immunofluorescent images is presented as well as a reference diagram of mammalian cell cycle. Scale bar = 25 μm. (b) Beas-2B cells treated with B[a]P were assessed for WST1 metabolism. n = 5. (c) Quantitative EdU uptake among EdU + Beas-2B cells treated with B[a]P. n = 4. (d) Cumulative cell cycle phase distributions of Beas-2B cells treated with B[a]P or cell cycle controls are presented. n = 4, except nocodazole-treated Beas-2B for which n = 2. (e) Magnitude changes compared to untreated controls among each categorical cell cycle phase are presented; bar colors coincide with phase designations presented in (d). n = 4, except nocodazole-treated Beas-2B for which n = 2. Positive changes denote increased percentage of cells in a specific phase among treated cells, whilst negative values denote decreased percentage. Endpoints are the arithmetic mean of listed independent experiments with error bars denoting one standard error of the mean. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37217524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22125_mouse-monoclonal-glucose-6-phosphate-dehydrogenase-antibody-2h7-310202416114769.jpg)