| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56153SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47781-0.1mg | |||
| IHC-Prediluted | NBP2-48351 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-207SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86912-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2521SS | |||
![Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Western-Blot-NB120-11512-img0006.jpg "Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cell extract separated on SDS-PAGE and probed with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1. Detection by Rabbit Anti-Rat IgG-Peroxidase and a chemiluminescent substrate. Antibody dilution 1:250.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Adult mouse small intestine stained with NB12-11512. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0009.jpg)
![Western Blot: E-Cadherin Antibody (DECMA-1) [NB120-11512] - Cat testis, cat ovary and HEK293 whole cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Western-Blot-NB120-11512-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:3200 Rat Monoclonal Anti-Uvomorulin/E-Cadherin, Clone: DECMA-1. Detection using Goat Anti-Rat IgG, FITC conjugate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (DECMA-1) [NB120-11512] - MDCK cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Rat Anti-Uvomorulin/E-Cadherin Clone: DECMA-1 diluted to 1:3200. The antibody was developed using Rabbit Anti-Rat IgG, FITC-conjugate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-DECMA-1-Immunocytochemistry-Immunofluorescence-NB120-11512-img0008.jpg)
![Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.](http://images.novusbio.com/fullsize/p35-baculovirus-Antibody-Western-Blot-NB100-56153-img0002.jpg "Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - 293T cells stained with Cyclin A2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunofluorescence-NBP1-31330-img0010.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0006.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - 30 ug PC-12 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0007.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0013.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Cyclin A2 Antibody [NBP1-31330] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0014.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin A2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Western-Blot-NBP1-31330-img0016.jpg)
![Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NBP1-31330] - NCIN87 Xenograft, using cyclin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunohistochemistry-Paraffin-NBP1-31330-img0004.jpg)
![Immunoprecipitation: Cyclin A2 Antibody [NBP1-31330] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of Cyclin A2 protein by 2. 5 ug of Cyclin A2 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/Cyclin-A2-Antibody-Immunoprecipitation-NBP1-31330-img0009.jpg)
![Western Blot: Cyclin A2 Antibody [NBP1-31330] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured by RNA-seq, qRT-PCR, & Western Blots. (a) Four genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; green: qRT-PCR; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; qRT-PCR: log 2− delta delta CT; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterisk: ∗p Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cyclin A2 stained by Cyclin A2 antibody (NBP1-31330) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-31330_rabbit-polyclonal-cyclin-a2-antibody-immunocytochemistry-immunofluorescence-141120251833319.jpg)
![Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Immunohistochemistry-Paraffin-NBP2-44525-img0006.jpg "Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Human Bladder Carcinoma stained with p21/CIP1/CDKN1A Antibody (DCS-60.2).")
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Simple Western lane view shows a specific band for p21/CIP1/CDKN1A in 0.2 mg/ml of MCF-7 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0002.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Electropherogram image of the corresponding Simple Western lane. p21/CIP1/CDKN1A antibody was used at 10 ug/ml dilution of MCF-7 lysates(s) respectively.](http://images.novusbio.com/fullsize/p21-CIP1-CDKN1A-Antibody-DCS-60-2-Simple-Western-NBP2-44525-img0003.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Analysis of human HeLa cell lysate using p21/CIP1/CDKN1A Antibody (DCS-60.2).](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720249362616.jpg)
![Simple Western: p21/CIP1/CDKN1A Antibody (DCS-60.2) [NBP2-44525] - Lane view shows a specific band for p21/CIP1/CDKN1A using 2 mg/mL of MCF-7 cell lysate and 10 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.](http://images.novusbio.com/fullsize/nbp2-44525_mouse-monoclonal-p21-cip1-cdkn1a-antibody-dcs-60-2-17720241044618.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0018.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0015.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0016.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0017.jpg)
![Western Blot: Patched 1/PTCH Antibody - BSA Free [NB200-118] - Analysis of Human PTCH1 in Fetal brain Lysate with anti huPTCH1 at a 1:500 dilution.](http://images.novusbio.com/fullsize/Patched-1-PTCH-Antibody---BSA-Free-Western-Blot-NB200-118-img0001.jpg)
![Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/IGF-I-Antibody-Immunohistochemistry-Paraffin-NBP2-16929-img0003.jpg "Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Western-Blot-NB500-207-img0001.jpg "Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.")
![Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Immunocytochemistry-Immunofluorescence-NB500-207-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody [NBP1-86912] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86912-img0007.jpg "Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody [NBP1-86912] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-194202485445..jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] -Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-194202485535..jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-19420248570..jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody [NBP1-86912] -Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86912_rabbit-polyclonal-trap-alpha-antibody-immunohistochemistry-paraffin-194202485812..jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TRAP alpha Antibody [NBP1-86912] - Analysis using Anti-SSR1 antibody NBP1-86912 (A) shows similar pattern to independent antibody NBP1-87815 (B).](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-Western-Blot-NBP1-86912-img0010.jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15039_rabbit-polyclonal-nfix-antibody-immunohistochemistry-paraffin-282023113015..jpg)
![Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus.NFIX stained by NFIX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15039_rabbit-polyclonal-nfix-antibody-immunohistochemistry-paraffin-282023113118..jpg)
![Immunohistochemistry-Paraffin: Speedy/Ringo Antibody [NB100-2521] - Speedy/Ringo was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human Speedy/Ringo polyclonal Antibody (Catalog # NB100-2521) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei of sperm cells.](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Immunohistochemistry-Paraffin-NB100-2521-img0004.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Detection of Speedy/Ringo in mouse testis (2ug/ml).](http://images.novusbio.com/fullsize/Speedy-Ringo-Antibody-Western-Blot-NB100-2521-img0003.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Elevated Spy1 levels prevent contact inhibition. (A) Representative views of focus formation assays in NIH3T3 cells transfected with Myc-Spy1-WT, Myc-Spy1-TST, positive control Ras-V12 or empty vector PCS3 control. n = 3 (B) Quantification of the number of colonies. Error bars reflect SE between triplicate experiments. t test was performed;** P ≤ 0.01. n = 3 (right panel) Western blot analysis of one representative lysate from A-B. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241534341.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 stable protein induces anchorage independent growth. (A) 293 cells were transfected with different concentrations of Myc-Spy1-WT, Myc-Spy1-TST or 30 μg of empty PCS3 vector control. Soft agar assay was carried out & plates photographed & quantified on day 14. Foci were averaged over 3 separate transfections for each experiment. n = 3 (B) Western blot analysis of one representative experiment; densitometry in lower panel. n = 3 (C) Representative foci after 14 day soft agar assay visualized using light microscopy. Ras-V12 is transfected as a positive control. n = 3. (D) Total numbers of foci were counted over 3 separate plates using separate transfections for each experiment. n = 3. Error bars reflect SE between triplicate experiments. t test was performed;** P ≤ 0.01. (E) Western blot analysis of experiments in Figure. 1C & D. Quantified using densitometry followed by normalization for Actin levels. (A-E) Error bars reflect SE between triplicate experiments. t test was performed;* P≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-310202415541987.jpg)
![Immunocytochemistry/ Immunofluorescence: Speedy/Ringo Antibody [NB100-2521] - CDC2 associates with SPDY at ERES. (A-C) 0 h GV stage oocyte labeled for CDC2 (A, green), DNA (A, blue), & SPDY (B, red). Both CDC2 & SPDY localize to the same cortical domain (C). Note that the center of this oocyte is dented (the area that contains the arrow) causing the structure to appear in the middle of the oocyte, whereas it is located in the cortex. (D-F) 0 h GV stage oocyte stained for Cyclin B (D, green), DNA (D, blue), & P-GM130 (E, red). Cyclin B did not localize to the P-GM130-labeled cortical domain (F). (G-I) 0 h GV stage oocyte labeled for PSTAIR (G, green), DNA (G, blue), & P-GM130 (H, red). Spatial overlap of PSTAIR & phosphorylated GM130 staining (yellow) in a cortical domain is evident in the merged image (I). Images are Z-projections of 2 (A-C) or 3 (D-I) consecutive sections; scale bars represent 20 μm in C, F, & I, & 5 μm in C', F', & I'. Arrows indicate the region of the oocyte that is shown enlarged in the insets (A'-I'). Arrowheads denote the position of the GV. Image collected & cropped by CiteAb from the following publication (//bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-9-8), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241555219.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 stable protein accelerates tumorigenesis in vivo.(A) Percentage of mice presenting with palpable tumors from 0-19 days post-transplant. Each data point represents 4 mice per indicated construct. The entire experiment was repeated three times using three independently derived overexpressing cell lines for each construct. Mann-Whitney Test was performed (p < 0.05). (A; lower blot) Western blots were conducted to measure the stability of Myc-Spy1-WT (left) & Myc-Spy1-TST (right) from representative time points. Empty vector control (Cntl) cell expression levels of Spy1 are seen in lane 1 of each blot. (B) Total tumor volume was calculated for both Spy1-HC11 (Spy1-WT) & Spy1-TST-HC11 (Spy1-TST) transplanted glands. Results were taken from 45 transplants using cells from 3 separate transfections. Error bars reflect SE between transplants from different transfections. Left hand panel reflects overall volume, right hand panel reflects volumes of tissues taken over a month post-transplant. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241553735.jpg)
![Western Blot: Speedy/Ringo Antibody [NB100-2521] - Spy1 protein levels are elevated in human breast cancers.(A) TMAs containing cores from invasive ductal carcinoma (IvDC), infiltrated ductal carcinoma (IfDC), intraductal carcinoma (IDC) & invasive lobular carcinoma (ILC) as well as pair-matched normal (PM-N) or cancer-free patients (CF-N) were analyzed for Spy1 expression. The Spy1 signal intensity was normalized to nuclear stain (TOTO-3/PI) signal. Patient numbers are indicated below the sample (N). (B) Breast normal & cancer cell lines were analyzed by western blot analysis. Actin was used as a loading control. One representative blot of 2. (C) Western blot of 3 replicate infections of pLKO control or pLKO Spy1 in MDA-231 cells. Middle panel represents the densitometry values over 3 separate experiments. Where Spy1 is corrected for actin levels. Lower panel reflects cell counts at 4, 6, & 24 h post-infection. (All panels) Data shown is mean ± s.d. Student's t-test was performed * P < 0.05;**P < 0.01. (D) Knockdown effects on cell counts of MCF7 cells over 72 h after transfection with either pSUPER empty vector (siCntl) or pSUPER-Spy1 (siSpy1) over 3 separate transfections. Data shown is mean ± s.d. Image collected & cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-45), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2521_rabbit-polyclonal-speedy-ringo-antibody-31020241555227.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
