| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74591 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-169SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44732 | |||
| Azide and BSA Free | NBP2-34700-0.1mg | |||
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Analysis in human testis and pancreas tissues using HPA002866 antibody. Corresponding SSBP1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: SSBP1 Antibody [NBP1-80720] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SSBP1 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Western-Blot-NBP1-80720-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SSBP1 Antibody [NBP1-80720] - Mitochondrial components segregate into MVBs and are secreted in exosomes. Confocal co-localization analysis in HEK293 cells treated with FCCP (4 h) and stained with SSBP1 (red), EEA1 and CD63 (green). Bar, 10 um. Graph shows means for Mander's co-localization coefficient for SSBP1 and EEA1 or CD63 (n = 3). t-test: ***P-value < 0.0001. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05077-9), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80720-img0023.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0020.jpg)
![Western Blot: SSBP1 Antibody [NBP1-80720] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Western-Blot-NBP1-80720-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SSBP1 Antibody [NBP1-80720] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80720-img0010.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human cerebral cortex shows granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0017.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0018.jpg)
![Immunohistochemistry-Paraffin: SSBP1 Antibody [NBP1-80720] - Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SSBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-80720-img0019.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Western-Blot-NB300-560-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP alpha.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Western-Blot-NBP2-01026-img0024.jpg "Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP alpha.")
![Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP alpha.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunocytochemistry-Immunofluorescence-NBP2-01026-img0015.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0023.jpg)
![Flow Cytometry: TRAP alpha Antibody (4C7) [NBP2-01026] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRAP alpha antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Flow-Cytometry-NBP2-01026-img0001.jpg)
![Western Blot: TRAP alpha Antibody (4C7) [NBP2-01026] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP alpha monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Western-Blot-NBP2-01026-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP alpha Antibody (4C7) [NBP2-01026] - Immunofluorescent staining of HeLa cells using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunocytochemistry-Immunofluorescence-NBP2-01026-img0014.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0016.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0017.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0018.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0019.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0020.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0021.jpg)
![Immunohistochemistry-Paraffin: TRAP alpha Antibody (4C7) [NBP2-01026] - Staining of paraffin-embedded Human pancreas tissue using anti-TRAP alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TRAP-alpha-Antibody-4C7-Immunohistochemistry-Paraffin-NBP2-01026-img0022.jpg)
![Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-16793-img0010.jpg "Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody, Internal (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HDAC4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/HDAC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16793-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Human colon carcinoma, using HDAC4 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HDAC4-Antibody-Immunohistochemistry-Paraffin-NBP2-16793-img0002.jpg)
![Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Histone Deacetylase 4/HDAC4 Antibody (NBP2-16793) diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Western-Blot-NBP2-16793-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-16793] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Histone-Deacetylase-4-HDAC4-Antibody-Knockdown-Validated-NBP2-16793-img0011.jpg)
![HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments.IP samples: Jurkat whole cell extractA. Control with 4 ug of preimmune Rabbit IgGB. Immunoprecipitation of HDAC4 protein by 4 ug HDAC4 antibody [N3C1], Internal (NBP2-16793)5 % SDS-PAGEThe immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (NBP2-16793) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-16793_rabbit-polyclonal-histone-deacetylase-4-hdac4-antibody-immunoprecipitation-2611202518461013.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (NBP2-16793) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16793_rabbit-polyclonal-histone-deacetylase-4-hdac4-antibody-western-blot-261120251845402.jpg)
![Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-H00124404-B01P-img0006.jpg "Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - An IFA revealed multiple localizations of SEPT12 and LAMINB1 signals. A total of 32 male germ cells were were evaluated as (a.) Round spermatids (n = 9; labeled with anti-SEPT12 and anti-LAMINB1 antibodies as the presented figure,9/9); (b.) elongating and (c.) elongated spermatids (n = 11;11/11); (d.) ejaculated spermatozoa (n = 10;10/10). (a.-d.) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), and a combination of the left and middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0007.jpg)
![Immunohistochemistry-Paraffin: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on formalin-fixed paraffin-embedded human colon. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunohistochemistry-Paraffin-H00124404-B01P-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - SEPT12 interacts & colocalizes with LAMINB1 male germ cells.(A) Co-IP of FLAG-SPAG4 & LAIMINB1 with SEPT12-GFP. The cell lysates of the NT2D1 cells, transfected with pEGFP-SEPT12 & a pFLAG-SPAG4 vector, are subjected to IP with an anti-GFP antibody (right panel, Row 3) or a nonspecific control IgG (right panel, Row 2), followed by IB with an anti-GFP, anti-FLAG, or anti-LAMINB1 antibody. An input protein (5%) was used as a control during the IB of the transfected cell lysates (right panel, Row 1). (B) An IFA revealed multiple localizations of SEPT12 & LAMINB1 signals. A total of 32 male germ cells were were evaluated as (A) Round spermatids (n = 9; labeled with anti-SEPT12 & anti-LAMINB1antibodies as the presented figure,9/9); (B) elongating & (C) elongated spermatids (n = 11;11/11); (D) ejaculated spermatozoa (n = 10;10/10). (A–D) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), & a combination of the left & middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00124404-b01p_mouse-polyclonal-septin-12-antibody-310202415483631.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human testis shows positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0018.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human liver shows very weak positivity in hepatocytes.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0015.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0016.jpg)
![Immunohistochemistry-Paraffin: Mkl1 Antibody [NBP1-88498] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Mkl1-Antibody-Immunohistochemistry-Paraffin-NBP1-88498-img0017.jpg)
![Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Western-Blot-NB100-74451-img0017.jpg "Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of various amounts of Human fibroblast whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0016.jpg)
![Western Blot: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of 10ug nuclear extract from fractionated mouse tissue lysate.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Western-Blot-NB100-74451-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (red) in HeLa cells at high magnification.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in HMVEC Cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C in A549 Cells.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (mab636) [NB100-74451] - Analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C, at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-mab636-Immunocytochemistry-Immunofluorescence-NB100-74451-img0015.jpg)
![Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Western-Blot-NB200-169-img0004.jpg "Western Blot: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Analysis of endogenous p19Arf using NB200-169. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (Lanes 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (Lanes 2), were resolved by SDS-PAGE. The membrane was probed with 1ug/ml purified p19Arf (NB200-169).")
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with NB200-169, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Immunocytochemistry-Immunofluorescence-NB200-169-img0003.jpg)
![Immunoprecipitation: p19ARF/CDKN2A Antibody (12-A1-1) [NB200-169] - Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (Lanes 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (Lanes 3), or NB200-169 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analysed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) from Arf null NIH3T3 cells (Lanes 1) and HA- p19Arf expressing cells (Lanes 2) were also analyzed by Western blotting, as input controls.](http://images.novusbio.com/fullsize/p19ARF-Antibody-12-A1-1-Immunoprecipitation-NB200-169-img0005.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Western-Blot-NB100-247-img0028.jpg "Western Blot: Claspin Antibody [NB100-247] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody NB100-247 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: Claspin Antibody [NB100-247] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Claspin-Antibody-Immunohistochemistry-NB100-247-img0027.jpg)
![Immunoprecipitation: Claspin Antibody [NB100-247] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Claspin,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Claspin-Antibody-Immunoprecipitation-NB100-247-img0023.jpg)
![Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Analysis using the Azide and BSA Free version of NBP2-32887. Detection of ODC1 in human placental lysate](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Western-Blot-NBP2-32887-img0004.jpg "Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Analysis using the Azide and BSA Free version of NBP2-32887. Detection of ODC1 in human placental lysate")
![Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - IF staining of LNCap cells using AF488 labeled ODC1 Monoclonal Antibody (ODC1/485) (GREEN) DAPI was used to stain the cell nucleus (blue). Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunocytochemistry-Immunofluorescence-NBP2-32887-img0014.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/485).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0012.jpg)
![Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Flow Cytometric Analysis of human ODC-1 in PC3 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ornithine Decarboxylase antibody (ODC1/485).](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Flow-Cytometry-NBP2-32887-img0015.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human prostate carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0005.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human Testicular carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0006.jpg)
![Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Rat pancreas stained with ODC1 Monoclonal Antibody (ODC1/485)](http://images.novusbio.com/fullsize/Ornithine-Decarboxylase-Antibody-ODC1-485-Immunohistochemistry-Paraffin-NBP2-32887-img0009.jpg)