NFIX Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: NFIX Antibody [NBP2-15039] - Nfix is mainly expressed by anti-inflammatory MPs. Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected ...read more
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus. NFIX stained by NFIX antibody [N3C3] diluted at 1:500.
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus. NFIX stained by NFIX antibody [N3C3] diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NFIX Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.
Predicted Species
Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NFIX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
Use in Chip and ICC/IF, WB reported in scientific literature (PMID: 30266829).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-15039 in the following applications:

  • 2 publications
  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Xenopus laevis (90%). Mouse reactivity reported in (PMID: 30266829).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS pH7, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NFIX Antibody

  • CTF
  • NF1ACCAAT-box-binding transcription factor
  • NF1-X
  • NF-I/X
  • NFI-X
  • nuclear factor 1 X-type
  • Nuclear factor 1/X
  • nuclear factor I/X (CCAAT-binding transcription factor)
  • Nuclear factor I/X
  • TGGCA-binding protein

Background

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NFIX Antibody (NBP2-15039)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NFIX Antibody (NBP2-15039) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NFIX Antibody (NBP2-15039)

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Bioinformatics

Gene Symbol NFIX
Uniprot