p21/CIP1/CDKN1A Antibody (DCS-60.2) [Alexa Fluor® 350]

• Reactivity: Hu, Mu(-), Rt(-)
• Applications: WB, Flow, IHC, IHC-P, ICC/IF
• Clone: DCS-60.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

p21/CIP1/CDKN1A Antibody (DCS-60.2) [Alexa Fluor® 350] Summary

• Immunogen: Human recombinant p21 protein
• Localization: Nuclear
• Specificity: This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDKN1A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p21/CIP1/CDKN1A Antibody (DCS-60.2) [Alexa Fluor® 350]

• CAP20cyclin-dependent kinase inhibitor 1
• CDK-interacting protein 1
• CDKN1A
• CDKN1melanoma differentiation associated protein 6
• CIP1
• CIP1WAF1CDK-interaction protein 1
• cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• MDA6
• MDA-6
• Melanoma differentiation-associated protein 6
• p21
• p21CIP1
• p21Cip1/Waf1
• PIC1
• SDI1DNA synthesis inhibitor
• wild-type p53-activated fragment 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKN1A