p35 baculovirus Antibody

Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.

• Reactivity: Dr, Sw, V-Vi
• Applications: WB, IHC, IHC-Fr, IHC-P, IP, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p35 baculovirus Antibody Summary

• Immunogen: Full-length recombinant p35 baculovirus protein was used as immunogen.
• Specificity: p35 baculovirus may be observed as one or multiple bands on western blot: p35 baculovirus is cleaved during its reaction with caspases. This results in cleavage of p35; 10 and 25 kDa fragments have been described. Additional breakdown p35 products may also result.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen Assay Dependent
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry reported in scientific literature (PMID: 21514385).
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Multiple specie cross reactivity. Silkworm reactivity reported in scientific literature (PMID: 24486953). Drosophila reactivity reported in scientific literature (PMID: 30413561). Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for p35 baculovirus Antibody

• p35 Baculovirus
• p35-Baculovirus

Background

The baculoviruses are a large, diverse family of DNA viruses that have evolved a number of mechanisms to manipulate thier insect hosts. One of these is the ability to regulate apoptosis during infection by expressing proteins that can inhibit caspase activation, including the caspase inhibitor p35 and the inhibitor of apoptosis (IAP) proteins (reviewed in Clem, 2005; Clarke and Clem, 2003; and Iller, 1997). The p35 baculovirus protein strongly inhibits caspase enzymatic activity, and is the the most broadly acting caspase inhibitor protein known. p35 baculovirus forms essentially irreversible complexes with its target caspases in a process that is accompanied by the cleavage of p35, generating two fragments of approximately 10 kDa and 25 kDa. These cleavage fragments remain associated with caspases and thereby block caspase activity. The ability of p35 to inhibit caspases along with the central role of caspases in the apoptotic process enables p35 baculovirus to block apoptosis in a phylogentically broad range of cells, and in response to a wide variety of apoptotic induction signals. For example, over expression of p35 in mammalian, insect, and nematode cells results in resistance to apoptosis. This antibody recognizes p35 baculovirus; p35 baculovirus migrates at ~35 kDa on SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p35 baculovirus Antibody (NB100-56153)(10)

Publications using NB100-56153

• Arthurton L, Nahotko DA, Alonso J et al. Non-apoptotic caspase activation preserves Drosophila intestinal progenitor cells in quiescence EMBO Rep 2020-11-01 [PMID: 33135280] (IHC-Fr, Drosophila)
  
  Details:
  Immunohistochemical analysis of intestines of Drosophila injected with Dronc-KO plasmids.
• Baena-Lopez LA, Arthurton L, Bischoff M et al. Novel initiator caspase reporters uncover unknown features of caspase-activating cells. Development 2018-11-09 [PMID: 30413561] (IHC, Drosophila)
• Clemente-Ruiz M, Murillo-Maldonado JM, Benhra N et al. Gene Dosage Imbalance Contributes to Chromosomal Instability-Induced Tumorigenesis Dev. Cell 2016-02-08 [PMID: 26859353] (IHC)
• Dekanty A, Barrio L, Milan M. Contributions of DNA repair, cell cycle checkpoints and cell death to suppressing the DNA damage-induced tumorigenic behavior of Drosophila epithelial cells. Oncogene 2014-03-17 [PMID: 24632609] (IHC-P, Virus)
  
  Details:
  p35 transgenic drosophila. The specificity of the p35 antibody was validated in p35 transgenic drosophila by IHC (P). Baculovirus.
• Zhang J, He Q, Zhang CD et al. Inhibition of BmNPV replication in silkworm cells using inducible and regulated artificial microRNA precursors targeting the essential viral gene lef-11. Antiviral Res. 2014-04-01 [PMID: 24486953] (WB, Silkworm)
  
  Details:
  BmN-SWU1 silkworm cells, Fig 4C.
• Groth Casper, Sasamura Takeshi, Khanna Mansi R et al. Protein trafficking abnormalities in Drosophila tissues with impaired activity of the ZIP7 zinc transporter Catsup. Development. 2013-07-01 [PMID: 23785054]
• Podratz JL, Staff NP, Froemel D et al. Drosophila melanogaster: a new model to study cisplatin-induced neurotoxicity. Neurobiol Dis. 2011-08-01 [PMID: 21514385] (ICC)
  
  Details:
  ICC (brains from Drosophila bred to express recombinant baculoviral p35 and WT Drosophila brains), Fig 5B-C. Flies were fixed with a formaldehyde-octane fixative, see publication for details. Note: The specificity of the IMG-5740 p35 antibody was validate
• Tian J, Zhang X, Liang B et al. Expression of baculovirus anti-apoptotic genes p35 and op-iap in cotton (Gossypium hirsutum L.) enhances tolerance to verticillium wilt. PLoS One. 2010-12-03 [PMID: 21151969] (WB)
  
  Details:
  WB (leaves from cotton bred to express recombinant baculoviral p35 and WT cotton leaves), Fig A. Flies were fixed with a formaldehyde-octane fixative, see publication for details. Note: The specificity of the IMG-5740 p35 antibody was validated by WB in c
• Arthurton L, Nahotko DA, Alonso J, Baena-Lopez LA Non-apoptotic caspase-dependent regulation of enteroblast quiescence in Drosophila BioRxiv (IHC, Drosophila)
• Shen J The transcription factor spalt and human homologue SALL4 induce cell invasion via the dMyc-JNK pathway in Drosophila Biol Open 2020-02-25 [PMID: 32098783] (Virus)