Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands ...read more
Full-length recombinant p35 baculovirus protein was used as immunogen.
p35 baculovirus may be observed as one or multiple bands on western blot: p35 baculovirus is cleaved during its reaction with caspases. This results in cleavage of p35; 10 and 25 kDa fragments have been described. Additional breakdown p35 products may also result.
Test in a species/application not listed above to receive a full credit towards a future purchase.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry reported in scientific literature (PMID: 21514385).
34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-56153 in the following applications:
Multiple specie cross reactivity. Silkworm reactivity reported in scientific literature (PMID: 24486953). Drosophila reactivity reported in scientific literature (PMID: 30413561). Not yet tested in other species.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Neat whole antisera
0.05% Sodium Azide
Alternate Names for p35 baculovirus Antibody
The baculoviruses are a large, diverse family of DNA viruses that have evolved a number of mechanisms to manipulate thier insect hosts. One of these is the ability to regulate apoptosis during infection by expressing proteins that can inhibit caspase activation, including the caspase inhibitor p35 and the inhibitor of apoptosis (IAP) proteins (reviewed in Clem, 2005; Clarke and Clem, 2003; and Iller, 1997). The p35 baculovirus protein strongly inhibits caspase enzymatic activity, and is the the most broadly acting caspase inhibitor protein known. p35 baculovirus forms essentially irreversible complexes with its target caspases in a process that is accompanied by the cleavage of p35, generating two fragments of approximately 10 kDa and 25 kDa. These cleavage fragments remain associated with caspases and thereby block caspase activity. The ability of p35 to inhibit caspases along with the central role of caspases in the apoptotic process enables p35 baculovirus to block apoptosis in a phylogentically broad range of cells, and in response to a wide variety of apoptotic induction signals. For example, over expression of p35 in mammalian, insect, and nematode cells results in resistance to apoptosis. This antibody recognizes p35 baculovirus; p35 baculovirus migrates at ~35 kDa on SDS-PAGE.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for p35 baculovirus Antibody (NB100-56153)(10)
We have publications tested in 3 confirmed species: Drosophila, Silkworm, Virus.
We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, WB.
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