| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48300 | |||
| Azide and BSA Free | NBP2-76425 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34685-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48154 | |||
| Azide and BSA Free | NBP2-34634-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23489SS | |||
| Azide and BSA Free | NBP2-80702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47916-0.1mg | |||
| IHC-Prediluted | NBP2-48305 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56405SS | |||
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Confocal immunofluorescent analysis of HeLa cells using Cytokeratin, panMouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Immunocytochemistry-Immunofluorescence-NBP2-44368-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Confocal immunofluorescent analysis of HeLa cells using Cytokeratin, panMouse Monoclonal Antibody (PAN-CK) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei are counterstained with NucSpot (red).")
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin, pan Mouse Monoclonal Antibody (PAN-CK).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Immunohistochemistry-Paraffin-NBP2-44368-img0004.jpg)
![Flow Cytometry: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Flow Cytometric Analysis of human HeLa cells. Cytokeratin, pan Antibody (PAN-CK (Cocktail)). using followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Flow-Cytometry-NBP2-44368-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Human Skin stained with Cytokeratin, pan Monoclonal Antibody cocktail (PAN-CK).](http://images.novusbio.com/fullsize/Cytokeratin-pan-Antibody-PAN-CK-Cocktail-Immunohistochemistry-Paraffin-NBP2-44368-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Human breast cancer tissue stained for panCK (white) and counterstained with DAPI (blue). The Alexa Fluor 750 conjugate of this antibody was used (NBP2-76425AF750). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-44368_mouse-monoclonal-cytokeratin-pan-antibody-pan-ck-cocktail-1772024100325.jpg)
![Immunohistochemistry-Frozen: Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [NBP2-44368] - Human breast cancer tissue stained for panCK (purple) and counterstained with DAPI (blue). The Alexa Fluor 750 conjugate of this antibody was used. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/nbp2-44368_mouse-monoclonal-cytokeratin-pan-antibody-pan-ck-cocktail-1772024959414.jpg)
![Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Western Blot Analysis of human PC3 cell lysate using Moesin Antibody (MSN/491).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Western-Blot-NBP2-32875-img0014.jpg "Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Western Blot Analysis of human PC3 cell lysate using Moesin Antibody (MSN/491).")
![Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/491) [NBP2-32875] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunocytochemistry-Immunofluorescence-NBP2-32875-img0013.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Fomalin-paraffin Rat colon stained with Moesin MAb (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0010.jpg)
![Flow Cytometry: Moesin Antibody (MSN/491) [NBP2-32875] - Flow Cytometric Analysis of K562 cells using Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Flow-Cytometry-NBP2-32875-img0012.jpg)
![Western Blot: Moesin Antibody (MSN/491) [NBP2-32875] - Analysis using the Azide and BSA Free version of NBP2-32875. Detection of Moesin in human HT29 Cells.](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Western-Blot-NBP2-32875-img0006.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin human placenta stained with Moesin MAb (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0007.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin human Melanoma stained with Moesin Mab (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0008.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) [NBP2-32875] - Formalin-paraffin Rat Lung stained with Moesin MAb (MSN/491)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Immunohistochemistry-Paraffin-NBP2-32875-img0009.jpg)
![Simple Western: Moesin Antibody (MSN/491) [NBP2-32875] - Simple Western lane view shows a specific band for Moesin in 0.1 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Simple-Western-NBP2-32875-img0003.jpg)
![Simple Western: Moesin Antibody (MSN/491) [NBP2-32875] - Electropherogram image of the corresponding Simple western lane view. Moesin antibody was used at 1 ug/ml dilution on HUVEC lysate(s) respectively.](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-491-Simple-Western-NBP2-32875-img0004.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0011.jpg "Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0008.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0010.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed, paraffin-embedded human leiomyosarcoma (10X) stained with muscle specific actin MAb (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0004.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Simple Western lane view shows a specific band for pan Actin in 0.2 mg/ml of Muscle (Sk) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0005.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Electropherogram image of the corresponding Simple Western lane. pan Actin antibody was used at 10 ug/ml dilution of Muscle (Sk) lysates(s) respectively.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31733-img0020.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-31733] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 antibody [N1-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Human breast carcinoma. MYH9 antibody [N1-2], N-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-31733-img0015.jpg)
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug Rat2 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0017.jpg)
![Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP1-31733-img0019.jpg)
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-31733] - Mouse prostate. MYH9 antibody [N1-2], N-term diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-31733-img0014.jpg)
![Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP1-31733] - Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1-2], N-term. Western blot analysis was performed using MYH9 antibody [N1-2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunoprecipitation-NBP1-31733-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: non-muscle Myosin IIA Antibody [NBP1-31733] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31733_rabbit-polyclonal-non-muscle-myosin-iia-antibody-1610202419405784.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [N1-2], N-term (NBP1-31733) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31733_rabbit-polyclonal-non-muscle-myosin-iia-antibody-western-blot-1411202518354619.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mouse mAb in (1) A549 and (2) Jurkat cell lysates.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0003.jpg "Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mouse mAb in (1) A549 and (2) Jurkat cell lysates.")
![Immunohistochemistry: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis of paraffin-embedded human cerebellum tissues using DRP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Immunohistochemistry-NBP2-23489-img0005.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mAb in human DRP1 recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0001.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mAb in (1) HEK293 and (2) DRP1 hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0002.jpg)
![Immunohistochemistry: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis of paraffin-embedded human ovarian cancer tissues using DRP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Immunohistochemistry-NBP2-23489-img0004.jpg)
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44579) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Knockout-Validated-NBP2-44579-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44579) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunocytochemistry-Immunofluorescence-NBP2-44579-img0005.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0001.jpg)
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western Blot Analysis of PC-3 cell lysate using Moesin antibody (MSN/492).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Western-Blot-NBP2-44579-img0009.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0003.jpg)
![Flow Cytometry: Moesin Antibody (MSN/492) [NBP2-44579] - Flow Cytometric Analysis of K562 cells. Moesin Antibody (MSN/492) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Flow-Cytometry-NBP2-44579-img0004.jpg)
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western Blot Analysis of human Jurkat cell lysate using Moesin Antibody (MSN/492).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Western-Blot-NBP2-44579-img0006.jpg)
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/492)](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with anti-Moesin (NBP2-44579) antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining), and far-red (KO) channels was performed. Representative grayscale images of the red channel are shown, and WT and KO cells are outlined with a yellow and magenta dashed line, respectively. A schematic representation of the mosaic strategy used is shown on the right panel. Antibody dilution used: NBP2-44579 at 1/200. Bar = 10 um. Images and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Moesin-Antibody-MSN-492-Knockout-Validated-NBP2-44579-img0008.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.](http://images.novusbio.com/fullsize/SENP1-Antibody-Western-Blot-NB100-56405-img0002.jpg "Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.")
![Simple Western: SENP1 Antibody [NB100-56405] - Simple Western lane view shows a specific band for SENP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SENP1-Antibody-Simple-Western-NB100-56405-img0003.jpg)
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Immunohistochemistry-Paraffin-NBP2-14890-img0009.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Mouse intestinal tissue pulsed with BrdU. BrdU stained by BrdU antibody diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for 15 minutes by flow cytometry analysis.Black: Unlabelled sample was used as a control.Red: BrdU antibodyAcquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-Flow-Cytometry-NBP2-14890-img0004.jpg)