Moesin Antibody (MSN/491) - Azide and BSA Free

Images

 
Western Blot: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Western blot of HT29 Cell Lysate using Moesin MAb (MSN/491).
Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Moesin with Moesin Antibody (MSN/491) followed by Goat ...read more
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin Rat colon stained with Moesin MAb (MSN/491)
Flow Cytometry: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Flow Cytometric Analysis of K562 cells using Moesin Antibody (MSN/491) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin human placenta stained with moesin MAb (MSN/491)
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin human Melanoma stained with moesin MAb (MSN/491)
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Formalin-paraffin Rat lung stained with Moesin MAb (MSN/491)
Simple Western: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Simple Western lane view shows a specific band for Moesin in 0.1 mg/ml of HUVEC lysate. This experiment was performed under reducing ...read more
Simple Western: Moesin Antibody (MSN/491) - Azide and BSA Free [NBP2-34685] - Electropherogram image of the corresponding Simple western lane view. Moesin antibody was used at 1 ug/ml dilution on HUVEC lysate(s) ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
MSN/491
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Moesin Antibody (MSN/491) - Azide and BSA Free Summary

Immunogen
Recombinant full-length human Moesin protein
Specificity
Recognizes 78kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Simple Western
  • Western Blot 0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A or G prepared in 10 mM PBS WITHOUT BSA & azide.

Alternate Names for Moesin Antibody (MSN/491) - Azide and BSA Free

  • Membrane-organizing extension spike protein
  • moesin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-52977
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-67584
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-87757
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
NBP2-25160
Species: Bv, Ca, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, WB
NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, WB
H00002035-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
NB100-91273
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB300-536
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
7268-CT
Species: Hu
Applications: BA
NBP3-13106
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-32822
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-67327
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-38683
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB

Publications for Moesin Antibody (NBP2-34685) (0)

There are no publications for Moesin Antibody (NBP2-34685).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Moesin Antibody (NBP2-34685) (0)

There are no reviews for Moesin Antibody (NBP2-34685). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP2-34685). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Secondary Antibodies

 

Isotype Controls

Additional Moesin Products

Bioinformatics Tool for Moesin Antibody (NBP2-34685)

Discover related pathways, diseases and genes to Moesin Antibody (NBP2-34685). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Moesin Antibody (NBP2-34685)

Discover more about diseases related to Moesin Antibody (NBP2-34685).
 

Pathways for Moesin Antibody (NBP2-34685)

View related products by pathway.

PTMs for Moesin Antibody (NBP2-34685)

Learn more about PTMs related to Moesin Antibody (NBP2-34685).
 

Research Areas for Moesin Antibody (NBP2-34685)

Find related products by research area.

Blogs on Moesin

There are no specific blogs for Moesin, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Moesin Antibody (MSN/491) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol MSN