alpha-Synuclein Antibody

Images

 
Western Blot: Synuclein-alpha Antibody [NBP2-25146] - Crude extract of rat brain, showing strong band at 15 kDa.
Immunocytochemistry/ Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 ...read more
Immunocytochemistry/ Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

alpha-Synuclein Antibody Summary

Immunogen
Full length human alpha-Synuclein [UniProt# P37840]
Localization
Cytoplasm. Membrane. Nucleus. Cell junction > synapse. Note: Membrane-bound in dopaminergic neurons.
Clonality
Polyclonal
Host
Chicken
Gene
SNCA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This alpha-Synuclein antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~15 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-25146 in the following applications:

Reactivity Notes

Rat and human. Human data from customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
10mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Synuclein Antibody

  • alpha-Synuclein
  • Lewy body) 4
  • MGC110988
  • NACP
  • non A-beta component of AD amyloid
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • non-A4 component of amyloid
  • PARK1
  • PARK4
  • PD1
  • SNCA
  • synuclein, alpha (non A4 component of amyloid precursor)
  • Synuclein-alpha

Background

Alpha-Synuclein is a member of the synuclein family, the other two proteins being beta and gamma synuclein. Alpha-Synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo. Direct homologues of alpha-Synuclein are found in all vertebrates. Later work connected alpha-Synuclein with human brain pathology, when a protein originally identified as a component of NAC, the "Non-Amyloid beta Component of Alzheimer's disease amyloid" proved to be a peptide derived from alpha-Synuclein. The alpha-Synuclein protein is therefore sometimes known as NAC precursor or NACP. Further work showed that alpha-Synuclein is a major component of the Lewy bodies of Parkinson's disease and point mutations of alpha-Synuclein proved to be causative of some forms of familial Parkinson's disease. However, despite being discovered as a component of amyloid preparations, alpha-Synuclein is apparently not a major component of the senile plaques of Alzheimer's disease. Early onset Parkinson's disease may be caused by a duplication or triplication of one of the alpha-Synuclein genes. Alpha-Synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy (MSA) and amyotrophic lateral sclerosis (ALS). Alpha-Synuclein is heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern. The synuclein proteins appear to have little 3D structure in solution, and probably belong to the family of "intrinsically unstructured proteins" which only adopt a well-defined conformation when bound to other proteins or membrane lipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Synuclein Antibody (NBP2-25146) (0)

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Review for alpha-Synuclein Antibody (NBP2-25146) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-25146:
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ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry alpha-Synuclein NBP2-25146
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reviewed by:
Anonymous
ICC Human 12/23/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsData are obtained by immunocytochemistry, human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken–594 (1:1000) secondary antibody. The NB110-55376R was diluted 1:200. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics).
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Synuclein Antibody (NBP2-25146). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?
    • Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
12/23/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol SNCA
Entrez
OMIM
Uniprot