Moesin Antibody (MSN/492)

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Genetic Strategies: Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. ...read more
Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain ...read more
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/492)
Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western Blot Analysis of PC-3 cell lysate using Moesin antibody (MSN/492).
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/492)
Flow Cytometry: Moesin Antibody (MSN/492) [NBP2-44579] - Flow Cytometric Analysis of K562 cells. Moesin Antibody (MSN/492) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: Moesin Antibody (MSN/492) [NBP2-44579] - Western Blot Analysis of human Jurkat cell lysate using Moesin Antibody (MSN/492).
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/492)
Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. ...read more

Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KO
Clone
MSN/492
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Moesin Antibody (MSN/492) Summary

Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038 )
Localization
Cytoplasmic & Cell Surface
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Knockout Validated
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-44579 in the following applications:

  • WB
    1 publication

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47916)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Moesin Antibody (MSN/492)

  • Membrane-organizing extension spike protein
  • moesin

Background

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Moesin Antibody (NBP2-44579)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP2-44579). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSN
Uniprot