RelA/NFkB p65 Antibody (27F9.G4)

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Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary ...read more
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show ...read more
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Human tissues. showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, ...read more
Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - This antibody was used in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 ...read more
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific lot 18372 ...read more
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
27F9.G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

RelA/NFkB p65 Antibody (27F9.G4) Summary

Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RELA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:50000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:5000
  • Immunohistochemistry 1:200-1:600
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide is sold separately
Publications
Read Publications using NBP1-77815.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for RelA/NFkB p65 Antibody (27F9.G4)

  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NBP1-77815)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RelA/NFkB p65 Antibody (NBP1-77815) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77815AF405
Alexa Fluor 488 Labeled NBP1-77815AF488
Alexa Fluor 647 Labeled NBP1-77815AF647
Alexa Fluor 700 Labeled NBP1-77815AF700
DyLight 350 Labeled NBP1-77815UV
DyLight 405 Labeled NBP1-77815V
DyLight 488 Labeled NBP1-77815G
DyLight 680 Labeled NBP1-77815FR
DyLight 755 Labeled NBP1-77815IR

Additional RelA/NFkB p65 Products

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Discover related pathways, diseases and genes to RelA/NFkB p65 Antibody (NBP1-77815). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RelA/NFkB p65 Antibody (NBP1-77815)

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PTMs for RelA/NFkB p65 Antibody (NBP1-77815)

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez