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Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Pm, Pm, RbSpecies Glossary
Applications WB, IHC, IHC-P
Clone
PAN-CK (Cocktail)
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-76425AF350
Alexa Fluor 405
NBP2-76425AF405
Alexa Fluor 488
NBP2-76425AF488
Alexa Fluor 532
NBP2-76425AF532
Alexa Fluor 594
NBP2-76425AF594
Alexa Fluor 647
NBP2-76425AF647
Alexa Fluor 700
NBP2-76425AF700
Alexa Fluor 750
NBP2-76425AF750
Allophycocyanin
NBP2-76425APC
Biotin
NBP2-76425B
DyLight 350
NBP2-76425UV
DyLight 405
NBP2-76425V
DyLight 488
NBP2-76425G
DyLight 550
NBP2-76425R
DyLight 594
NBP2-76425DL594
DyLight 650
NBP2-76425C
DyLight 680
NBP2-76425FR
DyLight 755
NBP2-76425IR
FITC
NBP2-76425F
Janelia Fluor 549
NBP2-76425JF549
Janelia Fluor 646
NBP2-76425JF646
PE
NBP2-76425PE
PerCP
NBP2-76425PCP
Unconjugated
NBP2-44368-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44368-0.1mg
IHC-Prediluted
NBP2-48300
Azide and BSA Free
NBP2-76425
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Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [HRP] Summary

Immunogen
This Cytokeratin, pan Antibody (PAN-CK (Cocktail)) was developed against human epidermal keratin.
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 55 kDa (CK7); 52 kDa (CK8); 56.5 kDa (CK10); 53 kDa (CK13); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 46 kDa (CK17); 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.
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Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, IHC, IHC-P
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NBP2-34270
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Cytokeratin 5
Antibody (EP1601Y)
NB110-56916
Western Blot: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Expression of cytokeratins (CK) and GAPDH in HMGEC. Representative western blots show specific bands for GAPDH, CK1, Cytokeratin 5, CK6 and CK14. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128096) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - A431 cells.
Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (EP1601Y) [NB110-56916] - Cytokeratin 5 (EP1601Y) on prostate.
(8 Publications)
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Involucrin Antibody
NBP2-33742
Immunocytochemistry/Immunofluorescence: Involucrin Antibody [NBP2-33742] - Staining of human cell line HaCaT shows localization to nuclear bodies, cytosol & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Involucrin Antibody [NBP2-33742] - Reconstructed human epidermis tissues are embedded in paraffin. Antigen retrieval: with citrate buffer. Antibody at 1:100. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Involucrin Antibody [NBP2-33742] - Staining of human esophagus shows moderate to strong cytoplasmic positivity in squamous epithelial cells.
(1 Review)
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Applications: Simple Western, ICC/IF, IHC, IHC-P
Filaggrin Antibody
NBP1-87528
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Analysis in human skin and liver tissues. Corresponding Filaggrin RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Filaggrin Antibody [NBP1-87528] - Staining of human gastrointestinal, liver, skin and testis using Anti-Filaggrin antibody NBP1-87528 (A) shows similar protein distribution across tissues to independent antibody NBP1-87527 (B).
Immunocytochemistry/Immunofluorescence: Filaggrin Antibody [NBP1-87528] - Staining of human cell line HaCaT shows localization to vesicles.
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
Endostatin [HRP]
DNST0
(21 Publications)
Species: Hu
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NBP1-84730
Western Blot: OXSM Antibody [NBP1-84730] - Analysis in control (vector only transfected HEK293T lysate) and OXSM over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: OXSM Antibody [NBP1-84730] - Staining of human cell line A-431 shows localization to cytosol & mitochondria.
Immunohistochemistry-Paraffin: OXSM Antibody [NBP1-84730] - Staining of human cerebral cortex.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Cytokeratin 3
Antibody (KRT3/2130)
NBP2-79718
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 3 Antibody (KRT3/2130).
Immunohistochemistry-Paraffin: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Formalin-fixed, paraffin-embedded human Ovary stained with Cytokeratin 3 Antibody (KRT3/2130).
Protein Array: Cytokeratin 3 Antibody (KRT3/2130) [NBP2-79718] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 3 Antibody (KRT3/2130). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
Species: Hu
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Antibody (SN2001)
NBP2-67727
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Cytokeratin 13
Antibody (SN71-09)
NBP2-67689
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Applications: WB, ICC/IF, IHC, IHC-P
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NBP2-67559
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(1 Publication)
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(1 Review)
(6 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
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Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
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(38 Publications)
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Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY licence.
Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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(3 Reviews)
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Cytokeratin 8
Antibody
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Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
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(3 Publications)
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Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
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(S112B-14)
NBP2-12900
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Immunohistochemistry: Kir2.1 Antibody (S112B-14) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P, MiAr
Cytokeratin, pan
Antibody (PAN-CK (Coc ...
NBP2-76425H
Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [HRP]
Species: Hu, Mu, Rt, Ca, Ch, Pm, Pm, Rb
Applications: WB, IHC, IHC-P

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-76425AF350
Alexa Fluor 405 NBP2-76425AF405
Alexa Fluor 488 NBP2-76425AF488
Alexa Fluor 532 NBP2-76425AF532
Alexa Fluor 594 NBP2-76425AF594
Alexa Fluor 647 NBP2-76425AF647
Alexa Fluor 700 NBP2-76425AF700
Alexa Fluor 750 NBP2-76425AF750
Allophycocyanin NBP2-76425APC
Biotin NBP2-76425B
DyLight 350 NBP2-76425UV
DyLight 405 NBP2-76425V
DyLight 488 NBP2-76425G
DyLight 550 NBP2-76425R
DyLight 594 NBP2-76425DL594
DyLight 650 NBP2-76425C
DyLight 680 NBP2-76425FR
DyLight 755 NBP2-76425IR
FITC NBP2-76425F
HRP NBP2-76425H
Janelia Fluor 549 NBP2-76425JF549
Janelia Fluor 646 NBP2-76425JF646
PE NBP2-76425PE
PerCP NBP2-76425PCP
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Bioinformatics

Gene Symbol KRT