Home » AlphaB Crystallin/CRYAB » AlphaB Crystallin/CRYAB Antibodies » AlphaB Crystallin/CRYAB Antibody [DyLight 755]

AlphaB Crystallin/CRYAB Antibody [DyLight 755]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Gp, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2519AF350
Alexa Fluor 405
NB100-2519AF405
Alexa Fluor 488
NB100-2519AF488
Alexa Fluor 532
NB100-2519AF532
Alexa Fluor 594
NB100-2519AF594
Alexa Fluor 647
NB100-2519AF647
Alexa Fluor 700
NB100-2519AF700
Alexa Fluor 750
NB100-2519AF750
Biotin
NB100-2519B
DyLight 350
NB100-2519UV
DyLight 405
NB100-2519V
DyLight 488
NB100-2519G
DyLight 550
NB100-2519R
DyLight 594
NB100-2519DL594
DyLight 650
NB100-2519C
DyLight 680
NB100-2519FR
FITC
NB100-2519F
HRP
NB100-2519H
Janelia Fluor 549
NB100-2519JF549
Janelia Fluor 646
NB100-2519JF646
Unconjugated
NB100-2519
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AlphaB Crystallin/CRYAB Antibody [DyLight 755] Summary

Immunogen
A synthetic peptide made to an internal region of the human Crystallin AB protein (within residues 70-140). [Swiss-Prot P02511]
Localization
Cytoplasmic
Predicted Species
Rat (100%), Porcine (100%), Guinea Pig (100%), Rabbit (100%), Chicken (100%), Sheep (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRYAB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted to react with rat, sheep, rabbit, monkey, guinea pig, chicken, and pig based on 100% sequence homology. Immunogen sequence has 81% homology with Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for AlphaB Crystallin/CRYAB Antibody [DyLight 755]

  • alpha B crystallin
  • alpha(B)-crystallin
  • AlphaB Crystallin
  • alpha-crystallin B chain
  • CRYA2
  • CRYA2alpha crystallin B chain
  • CRYAB
  • crystallin, alpha B
  • CTPP2
  • Heat shock protein beta-5
  • heat-shock 20 kD like-protein
  • HSPB5
  • Renal carcinoma antigen NY-REN-27
  • Rosenthal fiber component

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KO
AlphaA
Crystallin/CRYAA Anti ...
NBP2-46354
Western Blot: AlphaA Crystallin/CRYAA Antibody (OTI3B12) [NBP2-46354] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAA.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) [NBP2-46354] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: AlphaA Crystallin/CRYAA Antibody (OTI3B12) [NBP2-46354] - Analysis of Carcinoma of Human thyroid tissue.
Species: Hu
Applications: WB, IHC, IF
Desmin Antibody
AF3844
Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(2 Reviews)
(13 Publications)
Species: Hu, Mu
Applications: IHC
HSPB8/HSP22
Antibody
NBP1-84945
Western Blot: HSPB8/HSP22 Antibody [NBP1-84945] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPB8 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: HSPB8/HSP22 Antibody [NBP1-84945] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: HSPB8/HSP22 Antibody [NBP1-84945] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
(1 Publication)
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Applications: WB, ICC/IF, IHC, IHC-P, KD
GFAP Antibody
NB300-141
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.
(12 Reviews)
(59 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
HSP20/HSPB6
Antibody (SD086-03)
NBP2-67815
Western Blot: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of Hsp20 on different lysates using anti-Hsp20 antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle
Immunohistochemistry-Paraffin: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Hsp20 antibody. Counter stained with hematoxylin.
Flow Cytometry: HSP20/HSPB6 Antibody (SD086-03) [NBP2-67815] - Analysis of Hela cells with Hsp20 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
HspA4 Antibody
(SD0861)
NBP2-67817
Western Blot: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: HspA4 Antibody (SD0861) [NBP2-67817] - Staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: HspA4 Antibody (SD0861) [NBP2-67817] - Analysis of paraffin-embedded rat brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
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Caspase-3 Antibody
(31A1067) - (Pro and ...
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.
Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.
Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.
(1 Review)
(153 Publications)
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
HSP10/EPF Antibody
[Unconjugated]
AF2584
Western blot shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by HRP‑conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP10/EPF at approximately 10 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Simple Western lane view shows lysates of DA3 mouse myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP10/EPF at approximately 9 kDa (as indicated) using 25 µg/mL of Goat Anti-Mouse HSP10/EPF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2584) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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CRYGD Antibody
(4E12)
H00001421-M03
Western Blot: CRYGD Antibody (4E12) [H00001421-M03] - Analysis of CRYGD expression in HepG2 (Cat # L019V1).
Sandwich ELISA: CRYGD Antibody (4E12) [H00001421-M03] - Detection limit for recombinant GST tagged CRYGD is approximately 0.03ng/ml as a capture antibody.
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CRYBB2 Antibody
(1F1)
H00001415-M02
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - Analysis of CRYBB2 expression in transfected 293T cell line by CRYBB2 monoclonal antibody (M02), clone 1F1.Lane 1: CRYBB2 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: CRYBB2 Antibody (1F1) [H00001415-M02] - analysis of CRYBB2 in stably transfected HEK293T cell lysate. Note: membrane was blocked overnight in 3% BSA in TBST at 4C to reduce the background. Image from verified customer review.
ELISA: CRYBB2 Antibody (1F1) [H00001415-M02] - Detection limit for recombinant GST tagged CRYBB2 is 3 ng/ml as a capture antibody.
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(1 Publication)
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gamma C Crystallin
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Western Blot: gamma C Crystallin Antibody [NBP1-32557] - (30 ug whole cell lysate) A:A431 12% SDS PAGE diluted at 1:1000
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Myotilin Antibody
NBP2-38361
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human testis.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skeletal muscle shows high expression.
Immunohistochemistry-Paraffin: Myotilin Antibody [NBP2-38361] - Staining of human skin shows low expression as expected.
Species: Hu
Applications: IHC, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
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(1 Publication)
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p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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alpha-Synuclein
Antibody
NBP2-15365
Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-15365] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] - Frozen-sectioned adult mouse hippocampus. Green: alpha Synuclein protein stained by alpha Synuclein antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.
(1 Review)
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Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AlphaB Crystallin/CRYAB Antibody (NB100-2519IR) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2519AF350
Alexa Fluor 405 NB100-2519AF405
Alexa Fluor 488 NB100-2519AF488
Alexa Fluor 532 NB100-2519AF532
Alexa Fluor 594 NB100-2519AF594
Alexa Fluor 647 NB100-2519AF647
Alexa Fluor 700 NB100-2519AF700
Alexa Fluor 750 NB100-2519AF750
Biotin NB100-2519B
DyLight 350 NB100-2519UV
DyLight 405 NB100-2519V
DyLight 488 NB100-2519G
DyLight 550 NB100-2519R
DyLight 594 NB100-2519DL594
DyLight 650 NB100-2519C
DyLight 680 NB100-2519FR
DyLight 755 NB100-2519IR
FITC NB100-2519F
HRP NB100-2519H
Janelia Fluor 549 NB100-2519JF549
Janelia Fluor 646 NB100-2519JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CRYAB