Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Pm, Pm, RbSpecies Glossary
Applications WB, Flow, IHC, IHC-P, ICC/IF
Clone
PAN-CK (Cocktail)
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

Cytokeratin, pan Antibody (PAN-CK (Cocktail)) [Janelia Fluor® 549] Summary

Immunogen
This Cytokeratin, pan Antibody (PAN-CK (Cocktail)) was developed against human epidermal keratin.
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 55 kDa (CK7); 52 kDa (CK8); 56.5 kDa (CK10); 53 kDa (CK13); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 46 kDa (CK17); 45 kDa (CK18) and 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, IHC-P, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Cytokeratin, pan Antibody (NBP2-76425JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-76425AF350
Alexa Fluor 405 NBP2-76425AF405
Alexa Fluor 488 NBP2-76425AF488
Alexa Fluor 532 NBP2-76425AF532
Alexa Fluor 594 NBP2-76425AF594
Alexa Fluor 647 NBP2-76425AF647
Alexa Fluor 700 NBP2-76425AF700
Alexa Fluor 750 NBP2-76425AF750
Allophycocyanin NBP2-76425APC
Biotin NBP2-76425B
DyLight 350 NBP2-76425UV
DyLight 405 NBP2-76425V
DyLight 488 NBP2-76425G
DyLight 550 NBP2-76425R
DyLight 594 NBP2-76425DL594
DyLight 650 NBP2-76425C
DyLight 680 NBP2-76425FR
DyLight 755 NBP2-76425IR
FITC NBP2-76425F
HRP NBP2-76425H
Janelia Fluor 549 NBP2-76425JF549
Janelia Fluor 646 NBP2-76425JF646
PE NBP2-76425PE
PerCP NBP2-76425PCP

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Array NBP2-76425JF549

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Bioinformatics

Gene Symbol KRT