Bromodeoxyuridine/BrdU Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - HeLa cells mock (left) and treated with 50 uM BrdU for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: BrdU ...read more
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - Paraffin-embedded mouse intestinal tissue pulsed with BrdU antibody.
Flow Cytometry: Bromodeoxyuridine/BrdU Antibody [NBP2-14890] - BrdU antibody detects BrdU protein from HeLa treated with 10 uM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4C for 15 minutes by flow cytometry ...read more

Product Details

Summary
Reactivity Hu, AllSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

Order Details

Bromodeoxyuridine/BrdU Antibody Summary

Immunogen
The exact sequence is proprietary.
Marker
Proliferation Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
IHC-Fr usage reported in scientific literature (PMID: 23453541).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-14890) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Bromodeoxyuridine/BrdU Products

Bromodeoxyuridine/BrdU NBP2-14890

Blogs on Bromodeoxyuridine/BrdU.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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