Product Search for caveolin: Novus Biologicals

NB100-615

Caveolin-1 Antibody (7C8) - BSA Free

(3 Reviews)

(39 Publications)

0.1 ml

NB100-615

Immunohistochemistry-Frozen: Caveolin-1 Antibody (7C8) [NB100-615] - Immunofluorescence of human adipose tissue. Primary antibody at 1:250. IHC-Fr image submitted by a verified customer review.

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Antibody at 1:200 dilution (ON incubation) on EaHy926 endothelial cell line showing a clear localization in lipid raft/membrane. Photo courtesy of Alberto Davalos, Yale School of Medicine.

Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Caveolin-1 [7C8] conjugated to Alexa Fluor 488 (NB100-615AF488) at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Caveolin 1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Flow Cytometry: Caveolin-1 Antibody (7C8) [NB100-615] - An intracellular stain was performed on HeLa cells with Caveolin-1 Antibody (7C8) NB100-615 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Reactivity:

Hu, Mu, Rt, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Sh

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-615AF350
Alexa Fluor 405	NB100-615AF405
Alexa Fluor 488	NB100-615AF488
Alexa Fluor 532	NB100-615AF532
Alexa Fluor 594	NB100-615AF594
Alexa Fluor 647	NB100-615AF647
Alexa Fluor 700	NB100-615AF700
Alexa Fluor 750	NB100-615AF750
Alexa Fluor Plus 405	NB100-615AFP405
Alexa Fluor Plus 488	NB100-615AFP488
Alexa Fluor Plus 555	NB100-615AFP555
Alexa Fluor Plus 594	NB100-615AFP594
Alexa Fluor Plus 647	NB100-615AFP647
Alexa Fluor Plus 680	NB100-615AFP680
Allophycocyanin	NB100-615APC
Biotin	NB100-615B
CoraFluor 1	NB100-615CL1
DyLight 350	NB100-615UV
DyLight 405	NB100-615V
DyLight 488	NB100-615G
DyLight 550	NB100-615R
DyLight 594	NB100-615DL594
DyLight 650	NB100-615C
DyLight 680	NB100-615FR
DyLight 755	NB100-615IR
FITC	NB100-615F
HRP	NB100-615H
Janelia Fluor 525	NB100-615JF525
Janelia Fluor 549	NB100-615JF549
Janelia Fluor 646	NB100-615JF646
Janelia Fluor 669	NB100-615JF669
mFluor Violet 450 SE	NB100-615MFV450
mFluor Violet 500 SE	NB100-615MFV500
mFluor Violet 610 SE	NB100-615MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-615SS
Azide and BSA Free	NBP2-80617

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NBP1-31116

Caveolin-2 Antibody - BSA Free

(1 Review)

(1 Publication)

0.1 ml

NBP1-31116

Western Blot: Caveolin-2 Antibody [NBP1-31116] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Caveolin-2 Antibody [NBP1-31116] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.

Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116] - IP samples: A549 whole cell extract A. 30 ug A549 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 ug Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Caveolin-2 antibody detects Caveolin-2 protein at membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Caveolin-2 protein stained by Caveolin-2 antibody (NBP1-31116) diluted at 1:500.Blue: Hoechst 33342 staining.

The S100 protein family affects HNSCC metastasis and is regulated by CAV2.A The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 and SCC25 HNSCC cell lines was evaluated by immunoblotting. B The expression of S100 family proteins in CAV2-control and CAV2-knockdown (shCAV2-1, shCAV2-2) SCC15 cell lines was evaluated by RT–qPCR. C Alterations in the invasive ability of SCC15 and SCC25 cells following siRNA interference of S100 protein family members. The invasive ability of SCC15 and SCC25 cells was detected using Transwell assays. *, **, *** and **** indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

CAV2 promotes HNSCC metastasis both in vitro and in vivo.Silencing CAV2 in SCC15 and SCC25 cells by transfection with two individual CAV2 shRNAs significantly decreased CAV2 expression, as detected by western blotting (A) and QT-PCR (B). C, D The silencing of CAV2 significantly inhibited the migration and invasion of SCC15 and SCC25 cells, as evaluated using Transwell assays. (Scale bar, 200 μm). E Images of the macroscopic lung tissues of tail vein-injected mice. F The lung metastasis nodules were counted, and the data from the shCAV2 and negative control groups are summarized. ‘****’ indicates ‘p < 0.0001’. G CAV2 IHC staining and H&E staining to assess lung metastasis in tail vein-injected mice. (Scale bars, 200 μm and 100 μm). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

High expression of CAV2 is associated with the poor prognosis of HNSCC patients.A IHC analysis of CAV2 levels in 211 human HNSCC samples (scale bar, 100 μm or 400 μm). B Kaplan–Meier survival curve showing the correlation of overall survival and disease-free survival with the CAV2 IHC score. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36114176), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Pm, Fe, Rb, RM, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Pm, Fe, Rb, RM, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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AF5736

Caveolin-1 Antibody [Unconjugated]

(6 Publications)

100 μg

AF5736

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). Specific bands were detected for Caveolin-1 at approximately 21 to 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin-1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells and A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Caveolin-1 at approximately 29 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Caveolin-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Caveolin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Caveolin-1 at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma parental cell line and Caveolin‑1 knockout U-87 MG cell line (KO). Nitrocellulose membrane was probed with Goat Anti-Human Caveolin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5736) followed by HRP-conjugated secondary antibody. A specific band was detected for Caveolin‑1 at approximately 20.4 kDa (as indicated) in the parental U-87 MG cell line, but is not detectable in knockout U-87 MG cell line. Primary antibody dilution used: 0.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5736U-100UG
Alexa Fluor 405	AF5736V-100UG
Alexa Fluor 488	AF5736G-100UG
Alexa Fluor 532	AF5736X-100UG
Alexa Fluor 594	AF5736T-100UG
Alexa Fluor 647	AF5736R-100UG
Alexa Fluor 700	AF5736N-100UG
Alexa Fluor 750	AF5736S-100UG
Alexa Fluor Plus 405	AF5736AFP405-100UG
Alexa Fluor Plus 488	AF5736AFP488-100UG
Alexa Fluor Plus 555	AF5736AFP555-100UG
Alexa Fluor Plus 594	AF5736AFP594-100UG
Alexa Fluor Plus 647	AF5736AFP647-100UG
Alexa Fluor Plus 680	AF5736AFP680-100UG
Allophycocyanin	AF5736A
Biotin	AF5736B
CoraFluor 1	AF5736CL1
DyLight 350	AF5736D
DyLight 405	AF5736E
DyLight 488	AF5736K
DyLight 550	AF5736L
DyLight 594	AF5736M
DyLight 650	AF5736W
DyLight 680	AF5736Y
DyLight 755	AF5736Z
FITC	AF5736F
HRP	AF5736H
Janelia Fluor 525	AF5736JF525
Janelia Fluor 549	AF5736I
Janelia Fluor 646	AF5736J
Janelia Fluor 669	AF5736JF669
PE	AF5736P
PerCP	AF5736C
mFluor Violet 450 SE	AF5736MFV450
mFluor Violet 500 SE	AF5736MFV500
mFluor Violet 610 SE	AF5736MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5736-SP

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AF5788

Caveolin-2 Antibody [Unconjugated]

(2 Publications)

100 μg

AF5788

Western blot shows recombinant human Caveolin-1, Caveolin-2 and Caveolin-3 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for Caveolin-2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin-2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue).

Caveolin-2 was detected in paraffin-embedded sections of human liver using Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Caveolin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Caveolin-2 was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caveolin‑2 was detected in immersion fixed L6 rat myoblast cell line, A549 human lung carcinoma cell line and C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5788) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF5788U-100UG
Alexa Fluor 405	AF5788V-100UG
Alexa Fluor 532	AF5788X-100UG
Alexa Fluor 594	AF5788T-100UG
Alexa Fluor 647	AF5788R-100UG
Alexa Fluor 700	AF5788N-100UG
Alexa Fluor 750	AF5788S-100UG
Alexa Fluor Plus 405	AF5788AFP405-100UG
Alexa Fluor Plus 488	AF5788AFP488-100UG
Alexa Fluor Plus 555	AF5788AFP555-100UG
Alexa Fluor Plus 594	AF5788AFP594-100UG
Alexa Fluor Plus 647	AF5788AFP647-100UG
Alexa Fluor Plus 680	AF5788AFP680-100UG
Allophycocyanin	AF5788A
Biotin	BAF5788
CoraFluor 1	AF5788CL1
DyLight 350	AF5788D
DyLight 405	AF5788E
DyLight 488	AF5788K
DyLight 550	AF5788L
DyLight 594	AF5788M
DyLight 650	AF5788W
DyLight 680	AF5788Y
DyLight 755	AF5788Z
FITC	AF5788F
HRP	AF5788H
Janelia Fluor 525	AF5788JF525
Janelia Fluor 549	AF5788I
Janelia Fluor 646	AF5788J
Janelia Fluor 669	AF5788JF669
PE	AF5788P
PerCP	AF5788C
mFluor Violet 450 SE	AF5788MFV450
mFluor Violet 500 SE	AF5788MFV500
mFluor Violet 610 SE	AF5788MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5788-SP

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NB110-5029

Caveolin-3 Antibody

(2 Publications)

100 μg

NB110-5029

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse skeletal muscle tissue.

Flow Cytometry: Caveolin-3 Antibody [NB110-5029] - Analysis of U-87 MG cells.

Immunocytochemistry/Immunofluorescence: Caveolin-3 Antibody [NB110-5029] - HeLa cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a polyclonal anti-Caveolin 3 antibody (cat# NB110-5029) at a dilution of 1:20 overnight at 4 degrees Celsius, washed with PBS and incubated with a DyLight488 conjugated secondary antibody. Caveolin 3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue).

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse heart tissue.

Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NB110-5029] - Normal biopsies of deparaffinized Mouse lymph node tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB300-509

Caveolin-1 Antibody

(4 Publications)

100μg

NB300-509

Reactivity:

Hu, Mu, Rt, Ca, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ha

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-75050

Human Caveolin-1 ELISA Kit (Colorimetric)

1 Kit

NBP2-75050

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.31 - 20 ng/mL

Sensitivity:

0.19 ng/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP1-28917

Caveolin-3 Antibody

(2 Publications)

0.1 mg

NBP1-28917

Western Blot: Caveolin-3 Antibody [NBP1-28917] - Mouse (A) and Rat (B) heart lysates (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Western Blot: Caveolin-3 Antibody [NBP1-28917] - Pig skeletal muscle (A) and heart (B) lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP2-52431

Caveolin-2 Antibody (5E9E2) - BSA Free

0.1 mg

NBP2-52431

Flow Cytometry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of A549 cells using CAV2 mouse mAb (green) and negative control (red).

Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against HEK293 (1) and CAV2 (AA: 1-86)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against human CAV2 (AA: 1-86) recombinant protein. (Expected MW is 35.9 kDa)

Immunohistochemistry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.

ELISA: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52431-0.025mg

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MAB6706

HAF007). A specific band was detected for Caveolin-3 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human heart tissue, human skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, and mouse heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Caveolin-3 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human heart tissue, human skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, and mouse heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caveolin-3 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Caveolin-3 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human Caveolin-3 Monoclonal Antibody (Catalog # MAB6706) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma of muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6706U-100UG
Alexa Fluor 405	FAB6706V-100UG
Alexa Fluor 488	FAB6706G-100UG
Alexa Fluor 532	FAB6706X-100UG
Alexa Fluor 594	FAB6706T-100UG
Alexa Fluor 647	FAB6706R-100UG
Alexa Fluor 700	FAB6706N-100UG
Alexa Fluor 750	FAB6706S-100UG
Alexa Fluor Plus 405	FAB6706AFP405-100UG
Alexa Fluor Plus 488	FAB6706AFP488-100UG
Alexa Fluor Plus 555	FAB6706AFP555-100UG
Alexa Fluor Plus 594	FAB6706AFP594-100UG
Alexa Fluor Plus 647	FAB6706AFP647-100UG
Alexa Fluor Plus 680	FAB6706AFP680-100UG
Allophycocyanin	FAB6706A
Biotin	FAB6706B
CoraFluor 1	FAB6706CL1
DyLight 350	FAB6706D
DyLight 405	FAB6706E
DyLight 488	FAB6706K
DyLight 550	FAB6706L
DyLight 594	FAB6706M
DyLight 650	FAB6706W
DyLight 680	FAB6706Y
DyLight 755	FAB6706Z
FITC	FAB6706F
HRP	FAB6706H
Janelia Fluor 525	FAB6706JF525
Janelia Fluor 549	FAB6706I
Janelia Fluor 646	FAB6706J
Janelia Fluor 669	FAB6706JF669
PE	FAB6706P
PerCP	FAB6706C
mFluor Violet 450 SE	FAB6706MFV450
mFluor Violet 500 SE	FAB6706MFV500
mFluor Violet 610 SE	FAB6706MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6706-SP

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NBP2-33431

Caveolin-2 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP2-33431

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP2-33431] - Staining in human lung and pancreas tissues using anti-CAV2 antibody. Corresponding CAV2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP2-33431] - Staining of human lung shows strong cytoplasmic and membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP2-33431] - Staining of human pancreas shows low expression as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-33431-25ul

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NBP3-15615

Caveolin-1 Antibody (10Y5L2)

100 ul

NBP3-15615

Immunohistochemistry-Paraffin: Caveolin-1 Antibody (9I9J8) [NBP3-15615] -Human lung using Caveolin-1 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western Blot: Caveolin-1 Antibody (10Y5L2) [Caveolin-1] - Western blot analysis of various lysates, using Caveolin-1 Rabbit mAb at 1:10000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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