Caveolin-2 Antibody (5E9E2) - BSA Free

Images

 
Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mouse mAb against A549 (1), 3T3-L1 (2), A431 (3) cell lysate.
Immunohistochemistry-Paraffin: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.
Flow Cytometry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of A549 cells using CAV2 mouse mAb (green) and negative control (red).
Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against HEK293 (1) and CAV2 (AA: 1-86)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis using CAV2 mAb against human CAV2 (AA: 1-86) recombinant protein. (Expected MW is 35.9 kDa)
Immunohistochemistry: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.
ELISA: Caveolin-2 Antibody (5E9E2) [NBP2-52431] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
Clone
5E9E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

Caveolin-2 Antibody (5E9E2) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CAV2 (AA: 1-86) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CAV2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Caveolin-2 Antibody (5E9E2) - BSA Free

  • CAV
  • CAV2
  • caveolae protein, 20-kD
  • caveolin 2 isoform a and b
  • caveolin 2
  • Caveolin2
  • Caveolin-2
  • MGC12294

Background

Caveolae are cholesterol/sphingolipid-rich microdomains of the plasma membrane that have been implicated in signal transduction and vesicular trafficking. Caveolins are a family of caveolae-associated integral membrane proteins (1). Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Caveolins 1 and 2 are similar in most respects, however, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell (2). Caveolin-2 is up-regulated in response to the mechanical injury of differentiated PC12 cells; up-regulation of caveolin-2 under these conditions is strictly dependent on continued treatment with NGF. Robust expression of caveolin-1 and -2 is also observed along the entire cell surface of DRG neurons, including high levels on growth cones. These findings demonstrate that neuronal cells express caveolins (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CAV2