Caveolin-3 Antibody

Images

 
Western Blot: Caveolin-3 Antibody [NBP1-28917] - Analysis of Caveolin 3 in Human Heart lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Caveolin-3 Antibody [NBP1-28917] - (3.8ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Caveolin-3 Antibody [NBP1-28917] - Analysis of Mouse (A) and Rat (B) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Caveolin-3 Antibody [NBP1-28917] - Staining of Pig Skeletal Muscle (A) and Heart (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Caveolin-3 Antibody Summary

Immunogen
Peptide with sequence EHTDLEAQIVKDIH-C corresponding to N-Terminus according to NP_203123.1.
Epitope
N Terminus
Marker
Muscle Specific Caveolae Marker, T-tubule Marker
Specificity
Reported variants represent identical protein (NP_001225.1; NP_203123.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CAV3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 23 kDa band observed in human, mouse and rat heart lysates and 20 kDa band in porcine heart and skeletal muscle lysates (calculated MW of 17.3 kDa band according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources. IHC-P: Human skeletal muscle shows peripheral staining of fibres.
Publications
Read Publication using NBP1-28917.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Caveolin-3 Antibody

  • CAV3
  • caveolin 3
  • Caveolin3
  • Caveolin-3
  • LGMD1C
  • LGMD1CMGC126100
  • LQT9
  • LQT9MGC126101
  • M-caveolin
  • MGC126129
  • VIP21
  • VIP-21

Background

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caveolin-3 Antibody (NBP1-28917)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CAV3
Entrez
Uniprot