ZWINT Antibody

Images

 
Western Blot: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 on HeLa whole cell lysate using NB100-2364. ZWINT-1 was also immunoprecipitated by rabbit anti-ZWINT-1 antibody NB100-2363.
Immunocytochemistry/ Immunofluorescence: ZWINT Antibody [NB100-2364] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated ...read more
Immunohistochemistry-Paraffin: ZWINT Antibody [NB100-2364] - Detection of Human ZWINT-1 by IHC. Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:250.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

ZWINT Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 225 and the C-terminus (residue 277) of human ZW10 Interactor-1 using the numbering given in entry NP_008988.2 (GeneID 11130).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZWINT
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Positive Control
ZWINT Lysate (NBL1-18277)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZWINT Antibody

  • C20orf164
  • chromosome 20 open reading frame 164
  • dJ337O18.7
  • zinc finger SWIM domain-containing protein 3
  • zinc finger, SWIM domain containing 3
  • zinc finger, SWIM-type containing 3

Background

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZWINT Antibody (NB100-2364) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZWINT
Entrez
Uniprot