ZNHIT3 Antibody (2F8)

Western Blot: ZNHIT3 Antibody (2F8) [H00009326-M09] - Analysis of ZNHIT3 expression in HepG2 (Cat # L019V1).

Immunocytochemistry/Immunofluorescence: ZNHIT3 Antibody (2F8) [H00009326-M09] - Analysis of monoclonal antibody to ZNHIT3 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: ZNHIT3 Antibody (2F8) [H00009326-M09] - Detection limit for recombinant GST tagged ZNHIT3 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, S-ELISA
• Clone: 2F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ZNHIT3 Antibody (2F8) Summary

• Immunogen: ZNHIT3 (AAH17931, 1 a.a. ~ 155 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASLKCSTVVCVICLEKPKYRCPACRVPYCSVVCFRKHKEQCNPETRPVEKKIRSALPTKTVKPVENKDDDDSIADFLNSDEEEDRVSLQNLKNLGESATLRSLLLNPHLRQLMVNLDQGEDKAKLMRAYMQEPLFVEFADCCLGIVEPSQNEES
• Specificity: Reacts with zinc finger, HIT type 3.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNHIT3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZNHIT3 Antibody (2F8)

• HNF-4a coactivator
• Thyroid hormone receptor interactor 3TR-interacting protein 3
• Thyroid receptor-interacting protein 3
• TRIP-3
• TRIP3thyroid receptor interacting protein 3
• zinc finger HIT domain-containing protein 3
• zinc finger, HIT domain containing 3
• zinc finger, HIT type 3
• zinc finger, HIT-type containing 3

Background

ZNHIT3/TRIP3 interacts with HNF-4 alpha, a transcription factor involved in glucose metabolism. As a protein associated with HNF-4, ZNHIT3/TRIP3 functions as a transcriptional coactivator. Alternate names for ZNHIT3/TRIP3 include Zinc finger HIT domain-containing protein 3, thyroid receptor-interacting protein 3, and HNF4a coactivator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNHIT3
• Entrez:
  • 9326(Human)
• Uniprot:
  • AAH17931(Human)