ZNF521 Antibody - BSA Free

Western Blot: ZNF521 Antibody [NBP2-41174] - Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP2-41174] - Immunocytochemistry of ZNF521 (NT) in HeLa cells with ZNF521 (NT) antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody - BSA Free [NBP2-41174] - Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZNF521 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521. The immunogen is located within the first 50 amino acids of ZNF521. Amino Acid Squence: KKRPEDGEELEDEAVHS
• Specificity: ZNF521 antibody is human specific.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF521
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/mL
  • Western Blot 1 ug/mL
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZNF521 Antibody - BSA Free

• EHZF
• Evi3
• LIP3
• ZFP521
• zinc finger protein 263
• Zinc finger protein FPM315
• Zinc finger protein with KRAB and SCAN domains 12

Background

Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF521