ZNF521 Antibody [DyLight 650]

Images

 
There are currently no images for ZNF521 Antibody (NBP1-91271C).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

ZNF521 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to an internal portion of the human ZNF521 protein (between residues 450-550) [UniProt Q96K83]
Localization
Nucleus and cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZNF521
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This ZNF521 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot analysis.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ZNF521 Antibody [DyLight 650]

  • EHZF
  • Evi3
  • LIP3
  • ZFP521
  • zinc finger protein 263
  • Zinc finger protein FPM315
  • Zinc finger protein with KRAB and SCAN domains 12

Background

Zinc finger protein 521 (ZNF521; EHZF) is an 1131 amino-acid long transcription factor that includes 30 zinc fingers which are scattered along its sequence. Zfp521 appears to play a role in development, bone formation, erythroid cell differentiation, stem cell regulation and oncogenesis. Zfp521 is expressed in developing bones, where it binds to Runx2, thusly repressing the transcriptional activity of Runx2. This balance between Zfp521 and Runx2 may play a role in the regulation of osteoblast differentiation and bone formation (PMID: 20392215). Zinc finger protein 521 has also been shown to be expressed in hematopoietic stem and progenitor cells but is down-regulated as they go through the process of differentiation (PMID:19342626). Furthermore, ZNF521 was shown to inhibit the activity of GATA-1 which is a crucial regulator of erythroid differentiation by directly binding to GATA-1 (PMID: 19049973). Expression of ZNF521 has been detected in most acute myelogenous leukaemias, as well as in medulloblastomas and other brain tumors (PMID: 17543573).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5165
Species: Hu, Mu
Applications: WB
H00055835-M02
Species: Hu
Applications: WB, ELISA, IP
21720002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-82845
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92451
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-31310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-13611
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-57466
Species: Hu
Applications: ICC/IF
355-BM/CF
Species: Hu, Mu, Rt
H00010798-R02
Species: Hu
NB600-248
Species: Hu
Applications: WB, IP
NBP2-35213
Species: Hu
NBP1-86745
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-56505
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
MAB17791
Species: Hu, Mu
Applications: WB
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)

Publications for ZNF521 Antibody (NBP1-91271C) (0)

There are no publications for ZNF521 Antibody (NBP1-91271C).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ZNF521 Antibody (NBP1-91271C) (0)

There are no reviews for ZNF521 Antibody (NBP1-91271C). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for ZNF521 Antibody (NBP1-91271C) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ZNF521 Products

Bioinformatics Tool for ZNF521 Antibody (NBP1-91271C)

Discover related pathways, diseases and genes to ZNF521 Antibody (NBP1-91271C). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ZNF521 Antibody (NBP1-91271C)

Discover more about diseases related to ZNF521 Antibody (NBP1-91271C).
 

Pathways for ZNF521 Antibody (NBP1-91271C)

View related products by pathway.

Research Areas for ZNF521 Antibody (NBP1-91271C)

Find related products by research area.

Blogs on ZNF521

There are no specific blogs for ZNF521, but you can read our latest blog posts.

Customers Who Bought This Also Bought

BMP-2 [Unconjugated]
355-BM-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ZNF521 Antibody [DyLight 650] and receive a gift card or discount.

Bioinformatics

Gene Symbol ZNF521