ZNF521 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP1-91271] - Zfp521 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: ZNF521 Antibody [NBP1-91271] - IHC analysis of ZNF521 in mouse intestine using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.11 mg/ml

ZNF521 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human ZNF521 protein (between residues 450-550) [UniProt Q96K83]
• Localization: Nucleus and cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF521
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: This ZNF521 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF nuclear staining was observed in U2OS cells. In IHC-P, staining was observed in the cytoplasm and nucleus of mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.11 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF521 Antibody - BSA Free

• EHZF
• Evi3
• LIP3
• ZFP521
• zinc finger protein 263
• Zinc finger protein FPM315
• Zinc finger protein with KRAB and SCAN domains 12

Background

Zinc finger protein 521 (ZNF521; EHZF) is an 1131 amino-acid long transcription factor that includes 30 zinc fingers which are scattered along its sequence. Zfp521 appears to play a role in development, bone formation, erythroid cell differentiation, stem cell regulation and oncogenesis. Zfp521 is expressed in developing bones, where it binds to Runx2, thusly repressing the transcriptional activity of Runx2. This balance between Zfp521 and Runx2 may play a role in the regulation of osteoblast differentiation and bone formation (PMID: 20392215). Zinc finger protein 521 has also been shown to be expressed in hematopoietic stem and progenitor cells but is down-regulated as they go through the process of differentiation (PMID:19342626). Furthermore, ZNF521 was shown to inhibit the activity of GATA-1 which is a crucial regulator of erythroid differentiation by directly binding to GATA-1 (PMID: 19049973). Expression of ZNF521 has been detected in most acute myelogenous leukaemias, as well as in medulloblastomas and other brain tumors (PMID: 17543573).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF521
• Entrez:
  • 25925(Human)
• Uniprot:
  • Q96K83(Human)