ZNF346 Antibody - BSA Free

Western Blot: ZNF346 Antibody [NBP3-12616] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: A549 Whole Cell LysateLane D: Jurakt Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:38 kDa

Immunohistochemistry-Paraffin: ZNF346 Antibody [NBP3-12616] - Staining of human ZNF346 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: ZNF346 Antibody [NBP3-12616] - Staining of human ZNF346 in human brain with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: ZNF346 Antibody [NBP3-12616] - Lane A:0.5 mg Jurakt Whole Cell Lysate4 uL anti-ZNF346 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ZNF346 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 32 kDaObserved band size :38 kDa

Immunocytochemistry/Immunofluorescence: ZNF346 Antibody [NBP3-12616] - Staining of ZNF346 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12616 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ZNF346 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human ZNF346 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZNF346
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ZNF346 Antibody - BSA Free

• KIAA0844ZNF365D
• MGC41821
• MGC87345
• Su48
• talanin
• UANprotein ZNF365
• zinc finger protein 365

Background

ZNF346 is encoded by this gene is a nucleolar, zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids, rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding, but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF346