ZEB1 Antibody (639914) [Alexa Fluor® 700]

• Applications: Flow
• Clone: 639914
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

ZEB1 Antibody (639914) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human ZEB1
  Glu430-Ser575
  Accession # P37275
• Specificity: Detects human ZEB1 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZEB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Zinc finger E-box-binding homeobox 1 (ZEB1; also transcription factor 8 (TCF-8)) is a 124 kDa member of the delta-EF1/ZFH-1 C2H2-type zinc finger family.  Human ZEB1 is 1124 amino acids (aa) in length. The protein contains seven C2H2-type zinc fingers and one homeobox DNA-binding domain. In addition, there are eight phosphoserines and one phosphothreonine. Residues 989-1124 make up a glutamine-rich area. Within aa 430-575, human ZEB1 shares 84% and 82% aa sequence identity with mouse and rat ZEB1, respectively. The protein is expressed in heart and skeletal muscle, and defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3, a rare disease involving metaplasia and overgrowth of the corneal endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ZEB1 Antibody (FAB6708N). (Showing 1 - 1 of 1 FAQ).

1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
   • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

Bioinformatics

• Gene Symbol: ZEB1