ZBTB7A/Pokemon Antibody - BSA Free

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761] - Detection of human ZBTB7/FBI-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody NB100-761 (lot NB100-761-3) used for IP at 3 ug per reaction. ZBTB7/FBI-1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-761-2) and rabbit anti-ZBTB7/FBI-1 antibody NB100-762. For blotting immunoprecipitated ZBTB7/FBI-1, NB100-761 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZBTB7A/Pokemon Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 200 and 250 of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB7A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:3000-1:15000
• Application Notes: IHC reactivity reported in (PMID:20471975).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZBTB7A/Pokemon Antibody - BSA Free

• DKFZp547O146
• Factor binding IST protein 1
• Factor that binds to inducer of short transcripts protein 1
• FBI-1
• FBI-1POK erythroid myeloid ontogenic factor
• FBI1TTF-I-interacting peptide 21
• HIV-1 inducer of short transcripts binding protein
• Leukemia/lymphoma-related factor
• LRF
• LRFHIV-1 1st-binding protein 1
• lymphoma related factor
• MGC99631
• Pokemon
• TIP21
• ZBTB7A
• ZBTB7zinc finger and BTB domain containing 7
• zinc finger and BTB domain containing 7A
• zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcriptsbinding protein
• zinc finger and BTB domain-containing protein 7A
• Zinc finger protein 857A
• ZNF857APOZ and Krueppel erythroid myeloid ontogenic factor

Background

Pokemon (POK Erythroid Myeloid Ontogenic factor) is part of the POK gene family that encodes proteins. It is an oncogene, but its role is unique in that it controls the activity of other oncogenes. POK proteins are critical in embryonic development, cellular differentiation, and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZBTB7A/Pokemon Antibody (NB100-761)(4)

Publications using NB100-761

• Choi SH, Kim MY, Yoon YS, et al. Hypoxia-induced RelA/p65 derepresses SLC16A3 (MCT4) by downregulating ZBTB7A Biochim Biophys Acta Gene Regul Mech 2019-07-01 [PMID: 31271899] (WB, Human)
• Dobson NR, Moore RT, Tobin JE et al. Leukemia/lymphoma-related factor regulates oligodendrocyte lineage cell differentiation in developing white matter. Glia 2012-09-01 [PMID: 22615173]
• Taneja P, Maglic D, Kai F et al. Critical roles of DMP1 in human epidermal growth factor receptor 2/neu-Arf-p53 signaling and breast cancer development. Cancer Res 2010-11-01 [PMID: 21062982]
• Aggarwal A, Hunter WJ 3rd, Aggarwal H et al. Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers Exp Mol Pathol 2010-10-01 [PMID: 20471975] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: ZBTB7A
• Entrez:
  • 51341(Human)
  • 16969(Mouse)
• Uniprot:
  • O95365()