ZBP1/DLM-1/DAI Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

ZBP1/DLM-1/DAI Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human ZBP1/DLM-1/DAI
  Ala2-Ile429
  Accession # Q9H171
• Specificity: Detects human ZBP1/DLM-1/DAI in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZBP1/DLM-1/DAI Antibody [Alexa Fluor® 750]

• C20orf183
• chromosome 20 open reading frame 183
• DAI
• DLM1
• DLM-1
• DLM1dJ718J7.3
• DNA-dependent activator of interferon regulatory factors
• DNA-dependent activator of IRFs
• Tumor stroma and activated macrophage protein DLM-1
• ZBP1 Z-DNA binding protein 1
• ZBP1
• Z-DNA binding protein 1
• Z-DNA-binding protein 1

Background

ZBP1 (Z-DNA binding protein 1; also DAI and DLM-1) is a 15-60 kDa cytosolic detector of intracellular DNA. In a TOLL-independent manner, ZBP1 detects cytoplasmic dsDNA and initiates INF-beta production. It does so by binding DNA, followed by multimerization and recruitment of TBK1 and IRF3. This is accompanied by a parallel interaction with RIP-1 and -3 that stimulates NF kappa B activity and cytokine production. Human ZBP1 is 429 amino acids (aa) in length. It contains two DNA-binding Z-regions (aa 6-72 and 103-168) plus a third DNA-binding region that likely lies in the region between aa 170-250. Multiple splice forms exist. One shows a deletion of aa 12-86, a second shows a 63 aa substitution for aa 87-429, a third contains a 24 aa substitution for aa 225-429, a fourth reveals a deletion of aa 87-109, while a fifth and sixth show 82 and 16 aa substitutions for aa 365-429, respectively. Full length human ZBP1 shares only 47% aa identity with mouse ZBP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.