ZAK Antibody

Images

 
Western Blot: ZAK Antibody [NBP1-33137] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: ZAK Antibody [NBP1-33137] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ZAK Antibody [NBP1-33137] - Paraffin-embedded Mahlarvu xenograft. ZAK antibody [N1N2], N-term dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ZAK Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human ZAK. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Mouse (99%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZAK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
ZAK Lysate (NBP2-66103)

Reactivity Notes

Zebrafish (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ZAK Antibody

  • AZK
  • EC 2.7.11
  • EC 2.7.11.25
  • HCCS-4
  • Human cervical cancer suppressor gene 4 protein
  • Leucine zipper- and sterile alpha motif-containing kinase
  • mitogen-activated protein kinase kinase kinase MLT
  • mixed lineage kinase 7
  • mixed lineage kinase with a leucine zipper and a sterile alpha motif
  • mixed lineage kinase-related kinase MRK-beta
  • Mixed lineage kinase-related kinase
  • MLK7
  • mlklak
  • MLK-like mitogen-activated protein triple kinase
  • MLK-related kinase
  • MLT
  • MLTK
  • MRKcervical cancer suppressor gene 4 protein
  • pk
  • protein kinase
  • sterile alpha motif and leucine zipper containing kinase AZK
  • Sterile alpha motif- and leucine zipper-containing kinase AZK

Background

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZAK Antibody (NBP1-33137). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the two ZAK antibodies: NBP1-90367 and NBP1-33137? According to the amino acid sequence, the molecular weight of ZAK-alpha is about 91KDa and ZAK-beta is about 51KDa. NBP1-90367 corresponds to ZAK aa 157-305, which is the common amino acid sequence, so it can detect ZAK-alpha and ZAK-beta simultaneously. In your datasheet, the predicted band size is 51KDa. Additionally, in your or my western blot, I can't detect band in 91KDa. There is only one band in 51KDa. Is it reasonable that the band locating in 51kDa is ZAK-beta?
    • Both of these antibodies were raised to the N-terminal region shared by both isoforms 1&2. NBP1-33137 was generated using a partial recombinant protein containing a sequence within aa 18-254 of the human form. NBP1-90367 was developed to partial recombinant protein corresponding to aa 157-305.Based on the immunogen sequence, both antibodies are predicted to recognize both isoform 1 and isoform 2.  There are two possible reasons why only one band shows on the WBs for each of these antibodies:1. In tested tissues only one isoform is expressed. According to uniprot (http://www.uniprot.org/uniprot/Q9NYL2#expression) Isoform 2 is the predominant form in all tissues, except for liver, in which isoform 1 is more highly expressed.2. These antibodies do indeed recognize only one isoform, despite shared immunogen sequence. We have not, however, tested these antibodies side by side on the same lysates, neither have we performed further specificity testing to rule out reactivity with the other isoform.Please also note that according to uniprot (http://www.uniprot.org/uniprot/Q9NYL2#sequences), there are actually 3 isoforms for MAP3K20, the third one is the shortest, yielding a protein of ~35kDa and NBP1-90367 is not going to recognize this isoform, but NBP1-33137 will.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZAK