XRCC3 Antibody (10F1/6)


Western Blot: XRCC3 Antibody (10F1/6) [NB100-180] - XRCC3 detected in HEK293 lysates using a 1:1,000 dilution of Protein A purified NB100-180. Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts ...read more

Product Details

Reactivity Hu, Mu(-), Rt, Bv, Pm, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
1 mg/ml

XRCC3 Antibody (10F1/6) Summary

His-tagged human XRCC3, over-expressed in E. coli [UniProt# O43542]
This is specific for XRCC3. NB100-180 does not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western analysis.
Predicted Species
Rat (93%), Primate (100%), Bovine (93%), Sheep (93%). Backed by our 100% Guarantee.
IgG1 Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This XRCC3 (10F1/6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. In WB, a band can be seen at ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
XRCC3 Lysate (NBL1-17915)
Read Publications using
NB100-180 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human. Does not work in mouse. Predicted to react with monkey based on 100% sequence homology. Immunogen has 93% homology to sheep, rat, and bovine.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.4)
0.1% Sodium Azide
1 mg/ml
Protein A purified

Alternate Names for XRCC3 Antibody (10F1/6)

  • CMM6
  • DNA repair protein XRCC3
  • X-ray repair complementing defective repair in Chinese hamster cells 3
  • X-ray repair cross-complementing protein 3


XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XRCC3 Antibody (NB100-180)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB100-180 Applications Species
Chun J, Buechelmaier ES, Powell SN. Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway. Mol Cell Biol 2013 Jan [PMID: 23149936] (Human) Human
Sage JM, Gildemeister OS, Knight KL. Discovery of a novel function for human Rad51: maintenance of the mitochondrial genome. J Biol Chem;285(25):18984-90. 2010 Jun 18. [PMID: 20413593]
Gildemeister OS, Sage JM, Knight KL. Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C. J Biol Chem;284(46):31945-52. 2009 Nov 13. [PMID: 19783859]
Shammas MA et al. Dysfunctional homologous recombination mediates genomic instability and progression in myeloma. Blood;113(10):2290-7. 2009 Mar 5. [PMID: 19050310]
Li, B et al. WRN controls formation of extrachromosomal telomeric circles and is required for TRF2DeltaB-mediated telomere shortening. Mol Cell Biol;28(6):1892-904. 2008 Mar. [PMID: 18212065]
Chan, N et al. Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance. Cancer Res;68(2):605-14. 2008 Jan 15. [PMID: 18199558]
Compton, SA et al. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res;67(4):1513-9. 2007 Feb 15. [PMID: 17308089]
Bennett BT, Knight KL. Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51. J Cell Biochem;96(6):1095-109. 2005 Dec 15. [PMID: 16215984] (WB, ICC/IF, Human) WB, ICC/IF Human
Fan, R et al. Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression. Cancer Res;64(23):8526-33. 2004 Dec 1. [PMID: 15574758] (WB, Human) WB Human
Forget, AL et al. Xrcc3 is recruited to DNA double strand breaks early and independent of Rad51. J Cell Biochem;93(3):429-36. 2004 Oct 15. [PMID: 15372620] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XRCC3 Antibody (NB100-180) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-180AF405
Alexa Fluor 488 Labeled NB100-180AF488
Alexa Fluor 647 Labeled NB100-180AF647
Alexa Fluor 700 Labeled NB100-180AF700
Biotin Labeled NB100-180B
DyLight 350 Labeled NB100-180UV
DyLight 405 Labeled NB100-180V
DyLight 488 Labeled NB100-180G
DyLight 550 Labeled NB100-180R
DyLight 650 Labeled NB100-180C
DyLight 680 Labeled NB100-180FR
DyLight 755 Labeled NB100-180IR
FITC Labeled NB100-180F
HRP Labeled NB100-180H

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Gene Symbol XRCC3