XRCC3 Antibody (10F1/6)

Western Blot: XRCC3 Antibody (10F1/6) [NB100-180] - XRCC3 detected in HEK293 lysates using a 1:1000 dilution of Protein A purified NB100-180. Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School.

Immunocytochemistry/Immunofluorescence: XRCC3 Antibody (10F1/6) [NB100-180] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-XRCC3 Antibody (10F1/6) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: XRCC3 Antibody (10F1/6) [NB100-180] - Staining of esophageal squamous cell carcinoma with XRCC3 antibody. IHC-P image submitted by a verified customer review.

Product Discontinued

XRCC3 Antibody (10F1/6) Summary

• Immunogen: His-tagged human XRCC3, over-expressed in E. coli [Uniprot: O43542]
• Localization: Nuclear
• Specificity: This is specific for XRCC3. NB100-180 does not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western analysis.
• Predicted Species: Rat (93%), Sheep (93%), Primate (100%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: XRCC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: In Western blot, a band can be seen at ~38 kDa.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  XRCC3 Overexpression Lysate

Reactivity Notes

Does not work in mouse. Predicted to react with monkey based on 100% sequence homology.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for XRCC3 Antibody (10F1/6)

• CMM6
• DNA repair protein XRCC3
• X-ray repair complementing defective repair in Chinese hamster cells 3
• X-ray repair cross-complementing protein 3

Background

XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XRCC3 Antibody (NB100-180)(10)

Publications using NB100-180

• Chun J, Buechelmaier ES, Powell SN. Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway. Mol Cell Biol 2013-01-01 [PMID: 23149936] (Human)
• Kim TH, Payne U, Zhang X, Iwanaga Y, Davey MP, Rosenbaum JT, Inman RD. Altered host:pathogen interactions conferred by the Blau syndrome mutation of NOD2. Rheumatol Int;27(3):257-62. 2007-01-01 [PMID: 20413593]
• Gildemeister OS, Sage JM, Knight KL. Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C. J Biol Chem;284(46):31945-52. 2009-11-13 [PMID: 19783859]
• Shammas MA, Shmookler Reis RJ, Koley H et al. Dysfunctional homologous recombination mediates genomic instability progression in myeloma. Blood 2009;113(10):2290-2297 [PMID: 19050310]
• Li B, Jog SP, Reddy S et al. WRN controls formation of extrachromosomal telomeric circles and is required for TRF2DeltaB-mediated telomere shortening. Mol Cell Biol 2008-03-01 [PMID: 18212065]
• Chan, N et al. Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance. Cancer Res;68(2):605-14. 2008-01-15 [PMID: 18199558]
• Compton, SA et al. Xrcc3 and Nbs1 are required for the production of extrachromosomal telomeric circles in human alternative lengthening of telomere cells. Cancer Res;67(4):1513-9. 2007-02-15 [PMID: 17308089]
• Bennett BT, Knight KL. Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51. J Cell Biochem;96(6):1095-109. 2005-12-15 [PMID: 16215984] (WB, ICC/IF, Human)
• Fan, R et al. Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression. Cancer Res;64(23):8526-33. 2004-12-01 [PMID: 15574758] (WB, Human)
• Forget, AL et al. Xrcc3 is recruited to DNA double strand breaks early and independent of Rad51. J Cell Biochem;93(3):429-36. 2004-10-15 [PMID: 15372620] (ICC/IF, Human)

Review for XRCC3 Antibody (NB100-180) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 09/30/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Esophageal squamous cell carcinoma
• Species: Human
• Lot: 10F1/6

Bioinformatics

• Gene Symbol: XRCC3