Western Blot: XRCC3 Antibody (10F1/6) [NB100-180] - XRCC3 detected in HEK293 lysates using a 1:1000 dilution of Protein A purified NB100-180. Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts ...read more
Immunocytochemistry/ Immunofluorescence: XRCC3 Antibody (10F1/6) [NB100-180] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells ...read more
38 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Does not work in mouse. Predicted to react with monkey based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
0.02% Sodium Azide
Protein A purified
Alternate Names for XRCC3 Antibody (10F1/6)
DNA repair protein XRCC3
X-ray repair complementing defective repair in Chinese hamster cells 3
X-ray repair cross-complementing protein 3
XRCC3 (X-ray repair cross-complementing group 3) belongs to RecA family and participates in HRR/homologous recombination repair pathway which is an "error free" DNA repair mechanism that utilizes information encoded by homologous sequence to repair double-strand breaks (DSBs), chromosomal fragmentation, translocations and deletions. XRCC3 is required for Rad51 assembly/stabilization and in HRR pathway, XRCC3 protein interacts with Rad51 protein for contributing to chromosome integrity maintainance, thereby preventing mutations which can lead to carcinogenesis. In addition to repairing DSBs, XRCC3 also plays a role repairing wide range of DNA damage arising from carcinogens. Moreover, XRCC3 can control mtDNA copy number under conditions of oxidative stress in the presence of RAD51 as well as RAD51C. It forms a part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3, and has been suggested to forms complex with FANCD2, BRCA2 and phosphorylated FANCG. XRCC3 also interacts with SWSAP1 and ZSWIM7 for executing homologous recombination repair and defective XRCC3 have been linked to susceptibility to breast cancer as well as cutaneous malignant melanoma type 6 (CMM6).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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