XLF Antibody

Western Blot: XLF Antibody [NB100-2258] - Detection of Human XLF on HeLa whole cell lysate using NB100-2258. XLF was also immunoprecipitated using rabbit anti-XLF antibody NB100-2257.

Immunohistochemistry-Paraffin: XLF Antibody [NB100-2258] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XLF used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

XLF Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 250 and the C-terminus (residue 299) of human XRCC4-Like Factor using the numbering given in entry NP_079058.1 (GeneID 79840).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NHEJ1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2 -5 ug/mg lysate
  • Western Blot 1:2000 -1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  XLF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XLF Antibody

• Cernunnos
• non-homologous end-joining factor 1
• nonhomologous end-joining factor 1
• Protein cernunnos
• XLFFLJ12610
• XRCC4-like factor

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XLF Antibody (NB100-2258)(4)

Publications using NB100-2258

• Warmerdam DO, Alonso-de Vega I, Wiegant WW et al. PHF6 promotes non-homologous end joining and G2 checkpoint recovery EMBO Rep. 2019-11-29 [PMID: 31782600] (Human)
• Dutrannoy V, Demuth I, Baumann U et al. Clinical variability and novel mutations in the NHEJ1 gene in patients with a Nijmegen breakage syndrome-like phenotype. Hum Mutat 2010-09-01 [PMID: 20597108] (WB, Human)
• Wu PY, Frit P, Malivert L et al. Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell. J Biol Chem 2007-11-01 [PMID: 17720816]
• Zha S, Alt FW, Cheng HL et al. Defective DNA repair and increased genomic instability in Cernunnos-XLF-deficient murine ES cells. Proc Natl Acad Sci U S A 2007-03-01 [PMID: 17360556]

Bioinformatics

• Gene Symbol: NHEJ1
• Entrez:
  • 79840(Human)
• Uniprot:
  • NP_079058.1(Human)