XIAP Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC207627 and based on accession number NM_001167. The protein contains a C-terminal DDK tag.
Homo sapiens X-linked inhibitor of apoptosis (XIAP), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for XIAP
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for XIAP Overexpression Lysate (Native)
- API3X-linked inhibitor of apoptosis protein
- apoptosis inhibitor 3
- baculoviral IAP repeat-containing 4
- baculoviral IAP repeat-containing protein 4
- E3 ubiquitin-protein ligase XIAP
- EC 6.3.2.-
- IAP-like protein
- Inhibitor of apoptosis protein 3
- X-linked IAP
- X-linked inhibitor of apoptosis
The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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Publications for XIAP Lysate (NBL1-17905) (0)
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Bioinformatics Tool for XIAP Lysate (NBL1-17905)
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Blogs on XIAP.