XCL1/Lymphotactin Antibody (80222) [Unconjugated]

• Reactivity: Mu
• Applications: WB, ELISA(Cap)
• Clone: 80222
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

XCL1/Lymphotactin Antibody (80222) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse XCL1/Lymphotactin
  Val22-Gly114
  Accession # AAA56752
• Specificity: Detects mouse XCL1/Lymphotactin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human XCL1/Lymphotactin is observed and no cross-reactivity with recombinant viral (rv) CMV UL147 is observed. In Western blots, 75% cross‑reactivity with rvCMV UL147 is observed and no cross-reactivity with recombinant human CCL1-3, 5, 7, 8, 11, 13-25, 28, recombinant mouse CCL1-7, 9, 11, 12, 15, 19, 21, 22, 24, 25, 27 or 28 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: XCL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse XCL1/Lymphotactin Biotinylated Antibody (Catalog number BAF486)
  Standard: Recombinant Mouse XCL1/Lymphotactin (Catalog number 486-LT)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XCL1/Lymphotactin Antibody (80222) [Unconjugated]

• ATAC
• ATACSmall-inducible cytokine C1
• C motif chemokine 1
• chemokine (C motif) ligand 1
• Cytokine SCM-1
• LPTNSCM-1-alpha
• LTNXC chemokine ligand 1
• Lymphotactin
• lymphotaxin
• SCM-1 alpha
• SCM-1
• SCM1A
• SCM-1a
• SCYC1SCM1
• single cysteine motif 1a
• small inducible cytokine subfamily C, member 1 (lymphotactin)
• XCL1

Background

Mouse XCL1, also known as Lymphotactin, and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse XCL1 encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of XCL1 is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for XCL1 has been mapped to chromosome 1 in both human and mouse. Recombinant mouse XCL1 has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

1. Kelner, G. et al. (1994) Science 266:1395.
2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XCL1/Lymphotactin Antibody (MAB486)(3)

Publications using MAB486

• E Domenjo-Vi, V Casella, R Iwabuchi, E Fossum, M Pedragosa, Q Castellví, P Cebollada, T Kaisho, K Terahara, G Bocharov, J Argilaguet, A Meyerhans XCR1+ DCs are critical for T�cell-mediated immunotherapy of chronic viral infections Oncogene, 2023-02-14;42(2):112123. 2023-02-14 [PMID: 36795562] (Flow Cytometry, Mouse)
• A Deczkowska, E David, P Ramadori, D Pfister, M Safran, B At The, A Giladi, DA Jaitin, O Barboy, M Cohen, I Yofe, C Gur, S Shlomi-Lou, S Henri, Y Suhail, M Qiu, S Kam, H Hermon, E Lahat, G Ben Yakov, O Cohen-Ezra, Y Davidov, M Likhter, D Goitein, S Roth, A Weber, B Malissen, A Weiner, Z Ben-Ari, M Heikenwäld, E Elinav, I Amit XCR1+ type 1 conventional dendritic cells drive liver pathology in non-alcoholic steatohepatitis Nature Medicine, 2021-05-20;0(0):. 2021-05-20 [PMID: 34017133] (Neutralization, Mouse)
• JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control Cell, 2018-02-08;172(5):1022-1037.e14. 2018-02-08 [PMID: 29429633] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: XCL1
• Uniprot:
  • AAA56752()