XBP1 Antibody

Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Western Blot: XBP1 Antibody [NBP2-20917] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] diluted at 1:1000.

• Reactivity: Hu, Mu, RM
• Applications: WB, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

XBP1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human XBP1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XBP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 30877450). Bovine (88%), Rat (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for XBP1 Antibody

• HTF
• TREB5
• X-box binding protein 1
• X-box-binding protein 1
• XBP1
• XBP-1
• XBP2
• XBP2Tax-responsive element-binding protein 5

Background

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for XBP1 Antibody (NBP2-20917)(5)

Publications using NBP2-20917

• Varghese D, Oommen D, John A, Ali B GRP78/BiP alleviates oxLDL-induced hepatotoxicity in familial hypercholesterolemia caused by missense variants of LDLR Research Square 2023-02-24 [PMID: 37248472] (WB, Human)
• Madhamanchi K, Madhamanchi P, Jayalakshmi S et al. Endoplasmic reticulum stress and unfolded protein accumulation correlate to seizure recurrence in focal cortical dysplasia patients Cell stress & chaperones 2022-10-18 [PMID: 36258150]
• Diaz-Bulnes P, Saiz ML, Corte-Iglesias V et al. Demethylation of H3K9 and H3K27 Contributes to the Tubular Renal Damage Triggered by Endoplasmic Reticulum Stress Antioxidants (Basel, Switzerland) 2022-07-12 [PMID: 35883846]
• Gao P, Chai Y, Song J et al. Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus PLoS Pathog. 2019-11-18 [PMID: 31738790]
• Ouyang Y, Xu L, Lv J et al. Porcine circovirus type 2 ORF5 protein induces endoplasmic reticulum stress and unfolded protein response in porcine alveolar macrophages Arch. Virol. 2019-03-15 [PMID: 30877450] (WB, Porcine)

Bioinformatics

• Gene Symbol: XBP1
• Uniprot:
  • P17861(Human)