XBP1 Antibody

Images

 
Western Blot: XBP1 Antibody [NBP1-77253] - HepG2 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: XBP1 Antibody [NBP1-77253] - HepG2 cells with XBP-1 antibody at 10 ug/ml.
Immunohistochemistry: XBP1 Antibody [NBP1-77253] - XBP1 in mouse liver tissue with XBP1 antibody at 2 ug/ml.
Immunohistochemistry: XBP1 Antibody [NBP1-77253] - XBP1 in human liver tissue with XBP1 antibody at 5 ug/ml.
Immunofluorescence: XBP1 Antibody [NBP1-77253] - XBP1 in human liver tissue with XBP1 antibody at 20 ug/ml.
Immunofluorescence: XBP1 Antibody [NBP1-77253] - XBP-1 in HepG2 cells with XBP-1 antibody at 20 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

XBP1 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 2 ug/ml
  • Immunofluorescence 20 ug/ml
Application Notes
XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL and immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
Control Peptide
XBP1 Peptide (NBP1-77253PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for XBP1 Antibody

  • HTF
  • TREB5
  • X-box binding protein 1
  • X-box-binding protein 1
  • XBP1
  • XBP-1
  • XBP2
  • XBP2Tax-responsive element-binding protein 5

Background

X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to XBP1 Antibody (NBP1-77253). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on XBP1.

Analysis of Total & pSer724 IRE1 alpha, the Sensor of ER Stress
Inositol-requiring protein 1/IRE1 alpha (also called Endoplasmic Reticulum to Nucleus Signaling 1/ERN1; predicted mol wt 110 kDa) is a serine-threonine protein kinase/endoribonuclease which plays a highly critical role in unfolded protein response/U...  Read full blog post.

IRE1 - an important sensor in the unfolded protein response pathway
During cellular stress the protein folding capacity of the ER is diminished. In order to maintain homeostasis and ensure proper protein folding cells activate the unfolded protein response (UPR), a signaling network consisting of sensors and effect...  Read full blog post.

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Bioinformatics

Gene Symbol XBP1
Entrez
Uniprot