WTAP Antibody

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Western Blot: WTAP Antibody [NB600-1346] - E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-WTAP )

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF (-)
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

WTAP Antibody Summary

Immunogen
Synthetic peptide: MTNEEPLPKK VRLSETDFKV MARDELILRW KQYEAYVQAL EGKYTDLNSN DVTGLRESEE KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV QQPSVAQLRS TMVDPAINLF FLKMKGELEQ TKDKLEQAQN ELSAWKFTPDS, corresponding to amino acids 1-151 of KIAA0105.
Localization
Nuclear and Nucleolar
Specificity
WTAP
Clonality
Polyclonal
Host
Chicken
Gene
WTAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
WB: Use at a dilution of 1/2000. Predicted molecular weight: 44 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Positive Control
WTAP Lysate (NBL1-17889)

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Expected to cross-react with Zebrafish (80% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WTAP Antibody

  • Female-lethal(2)D homolog
  • hFL(2)D
  • KIAA0105DKFZp686F20131
  • MGC3925
  • PNAS-132
  • pre-mRNA-splicing regulator WTAP
  • putative pre-mRNA splicing regulator female-lethal(2D)
  • Wilms tumor 1 associated protein
  • Wilms tumor 1-associating protein
  • Wilms' tumour 1-associating protein
  • WT1-associated protein

Background

Wilms' tumour 1 associating protein plays a role in both transcriptional and post transcriptional regulation of certain cellular genes. This protein is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of its gene results in three transcript variants, two of which encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WTAP
Entrez